Information within the mature LamB protein necessary for localization to the outer membrane of E coli K12.

Information within the mature LamB protein necessary for localization to the outer membrane of E coli K12. Research Abstract Details 

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Information within the mature LamB protein necessary for localization to the outer membrane of E coli K12. Abstract Text:

S A Benson,T J Silhavy,

It has been proposed that the efficient localization of the outer membrane protein LamB requires a functional signal sequence and at least two additional regions contained within the mature protein. We define these regions more precisely by deletion analysis, and we describe methods for cloning deleterious lacZ fusions onto high-copy-number plasmids and generating in-frame deletions. Analysis of the effects of a series of internal lamB deletions on the export of a LamB-LacZ hybrid protein and of the LamB protein itself indicates that necessary informational signal(s) required for localization lie at the amino-terminal end of the protein. In addition, our analysis indicates that there is a region of information close to or within the fusion joint of the largest lamB-lacZ fusion that increases the efficiency of the export process. A unique deletion that removes a protein segment from amino acid 70 to 200 appears to prevent proteolytic removal of the signal sequence. Nevertheless, the mutant protein is exported to the outer membrane.

Information within the mature LamB protein necessary for localization to the outer membrane of E coli K12. Publishing Authors By Initials

SA Benson,TJ Silhavy,

For similar genetic processes: recombination, genetic research abstracts see: genetic processes: recombination, genetic research

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Information within the mature LamB protein necessary for localization to the outer membrane of E coli K12. Journal Published:

PUBLICATION TYPE: Research Support, U.S. Gov't,

Journal: Cell

VOLUME: 32

Page Numbers: 1325-35

Journal Abbreviation: Cell

ISSN: 0092-8674

DAY: 27

MONTH: Apr

YEAR: 1983

Information within the mature LamB protein necessary for localization to the outer membrane of E coli K12. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 413066

Information within the mature LamB protein necessary for localization to the outer membrane of E coli K12. Keywords Mesh Terms:

KEYWORDS: Recombination, Genetic

MESH TERMS: metabolism

Chemical & Substance for Abstract: Information within the mature LamB protein necessary for localization to the outer membrane of E coli K12. Information

Substance Name: maltoporins

Registry Number: 0

Grant and Affiliation Information for Information within the mature LamB protein necessary for localization to the outer membrane of E coli K12.

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Country: UNITED STATES

AGENCY: United States NCI

GRANT: N01-CO-23909

ACRONYM: CO

MEDLINETA: Cell

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