Influence of two transfectors on delivery of 99mTc antisense DNA in tumor-bearing mice.

Influence of two transfectors on delivery of 99mTc antisense DNA in tumor-bearing mice. Research Abstract Details 

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Influence of two transfectors on delivery of 99mTc antisense DNA in tumor-bearing mice. Abstract Text:

Kayoko Nakamura,Yi Wang,Xinrong Liu,Atsushi Kubo,Donald J Hnatowich,

PURPOSE: The aim of the study is to determine whether delivery into tumor cells in vivo of a 99mTc-labeled antisense phosphorothioate DNA targeting the mdr1 mRNA improves after electrostatic complexation with the transmembrane transfector (TF) carriers Neophectin or jetPEI as was observed by us in vitro. METHODS: The biodistribution of the labeled antisense DNA before and after complexation with either TF was determined in nude mice bearing KB-G2 (Pgp++) tumors. RESULTS: Complexation with either TF resulted in significantly higher background radioactivity levels in almost all normal tissues and modest improvement in tumor accumulation at best. The tumor accumulation was lower compared to naked at six hours (0.34 and 0.23 vs. 0.40% ID/g) and modestly higher at 24-28 hours (0.15 and 0.15 vs. 0.12% ID/g) for Neophectin and jetPEI, respectively. That blood was less than 0.18% ID/g for both TFs even at six hours suggests that tumor accumulations may have suffered from rapid blood clearance. CONCLUSION: The results of this investigation show that because of the unfavorable pharmacokinetics of radiolabeled phosphorothioate DNAs when electrostatically complexed to jetPEI or Neophectin, neither TF appears to be useful in vivo despite favorable results in vitro. Future studies will devote greater consideration to the relative rates of tumor accumulation and blood clearance.

Influence of two transfectors on delivery of 99mTc antisense DNA in tumor-bearing mice. Publishing Authors By Initials

K Nakamura,Y Wang,X Liu,A Kubo,DJ Hnatowich,

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Influence of two transfectors on delivery of 99mTc antisense DNA in tumor-bearing mice. Journal Published:

PUBLICATION TYPE: Research Support, U.S. Gov't,

Journal: Molecular imaging and biology : MIB : the official

VOLUME: 8

Page Numbers: 188-92

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ISSN: 1536-1632

DAY: 1

MONTH: 05

YEAR: 2006

Influence of two transfectors on delivery of 99mTc antisense DNA in tumor-bearing mice. Information

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LANGUAGE: eng

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AFFILIATION: Department of Radiology, Keio University School of Medicine, 35 Shinanomachi, Shinjuku-ku, Tokyo, 160-8582, Japan. kumako@sc.itc.keio.ac.jp

Country: United States

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MEDLINETA: Mol Imaging Biol

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