Influence of murine maternal diabetes on placental morphology, gene expression, and function.

Influence of murine maternal diabetes on placental morphology, gene expression, and function. Research Abstract Details 

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Influence of murine maternal diabetes on placental morphology, gene expression, and function. Abstract Text:

Maternal diabetes causes placental and foetal abnormalities in both rat and humans; however, its effect is less well documented in the mouse. We used a standard approach to induce manifest diabetes in pregnant mice and assessed morphology, function and gene expression in the placentas isolated from these females. We found that diabetic placentas exhibit a consistent abnormal phenotype characterized by increased junctional zone cross sectional area. Lipid profiling of diabetic foetuses and placentas showed that the placental phenotypes do not compromise the lipid transport function of this organ. In a genome-wide survey of mRNA expression by using cDNA micro-arrays, we identified 118 ESTs, corresponding to 59 annotated genes, with differential expression in the diabetic placentas. A significant proportion of these known is involved in metabolism, immunity and defence, and signal transduction. In addition, we found two imprinted genes, Igf2 and Gatm, which exhibited altered expression. The expression of other imprinted genes, Peg1, Gtl2, Peg3, Igf2r and Grb10, was determined by quantitative RT-PCR. For all of these genes, slight changes in gene expression were observed between diabetic placentas and control placentas. Our study thus provides the basis for future work that will address gene action in the diabetic mouse placenta.

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Influence of murine maternal diabetes on placental morphology, gene expression, and function. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Archives of physiology and biochemistry

VOLUME: 114

Page Numbers: 99-110

Journal Abbreviation: Arch. Physiol. Biochem.

ISSN: 1381-3455

DAY: 19

MONTH: Apr

YEAR: 2008

Influence of murine maternal diabetes on placental morphology, gene expression, and function. Information

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LANGUAGE: eng

NlmUniqueID: 9510153

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Grant and Affiliation Information for Influence of murine maternal diabetes on placental morphology, gene expression, and function.

AFFILIATION: Department of Development and Genetics, Evolutionary Biology Center, Uppsala University, Norbyvagen 18A, Uppsala, Sweden.

Country: England

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MEDLINETA: Arch Physiol Biochem

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