Influence of gelatin complexation on cell proliferation activity and proteolytic resistance of basic fibroblast growth factor.

Influence of gelatin complexation on cell proliferation activity and proteolytic resistance of basic fibroblast growth factor. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Influence of gelatin complexation on cell proliferation activity and proteolytic resistance of basic fibroblast growth factor. Abstract Text:

Y Tabata,T Ishii,M Muniruzzaman,Y Hirano,Y Ikada,

The objective of this study is to investigate the influence of gelatin complexation on the biological activity of basic fibroblast growth factor (bFGF) and its resistance to trypsin digestion. When bFGF was mixed at 37 degrees C with acidic gelatin with an isoelectric point (IEP) of 5.0, the activity to promote in vitro proliferation of BHK cells became lower compared with that of free bFGF, in contrast to mixing with the basic gelatin with an IEP of 9.0. A maximum reduction in the bFGF activity was observed for the bFGF-gelatin complex prepared at a mixing molar ratio of 1/1. The bFGF activity of cell proliferation reduced at the initial period after mixing with the acidic gelatin at 37 degrees C, followed by no substantial change. Complexation with the acidic gelatin at 4 degrees C had no influence on the bFGF activity, irrespective of the bFGF/gelatin ratio and complexation time. The biological activity of bFGF was reduced by the trypsin treatment, but the reduced extent was suppressed through gelatin complexation at 37 degrees C. In an electrophoresis study, the protective effect of gelatin complexation on the trypsin digestion was also confirmed in terms of the molecular weight loss. It is possible that the complexing gelatin covers bFGF molecules, resulting in suppression of their interaction with the cell surface receptor as well as protection from their enzymatic attack.

Influence of gelatin complexation on cell proliferation activity and proteolytic resistance of basic fibroblast growth factor. Publishing Authors By Initials

Y Tabata,T Ishii,M Muniruzzaman,Y Hirano,Y Ikada,

For similar natural sciences: time: time factors research abstracts see: natural sciences: time: time factors research

PUBMED ID PMID:

MEDLINE DATE:

Influence of gelatin complexation on cell proliferation activity and proteolytic resistance of basic fibroblast growth factor. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Journal of biomaterials science. Polymer edition

VOLUME: 11

Page Numbers: 571-82

Journal Abbreviation:

ISSN: 0920-5063

DAY: 20

MONTH: 02

YEAR: 2000

Influence of gelatin complexation on cell proliferation activity and proteolytic resistance of basic fibroblast growth factor. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 9007393

Influence of gelatin complexation on cell proliferation activity and proteolytic resistance of basic fibroblast growth factor. Keywords Mesh Terms:

KEYWORDS: Time Factors

MESH TERMS: metabolism

Chemical & Substance for Abstract: Influence of gelatin complexation on cell proliferation activity and proteolytic resistance of basic fibroblast growth factor. Information

Substance Name: Gelatin

Registry Number: 9000-70-8

Grant and Affiliation Information for Influence of gelatin complexation on cell proliferation activity and proteolytic resistance of basic fibroblast growth factor.

AFFILIATION: Institute for Frontier Medical Sciences, Kyoto University, Japan. yasuhiko@frontier.kyoto-u.ac.jp

Country: NETHERLANDS

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: J Biomater Sci Polym Ed

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Influence of gelatin complexation on cell proliferation activity and proteolytic resistance of basic fibroblast growth factor Related Publications

• A bioabsorbable delivery system for antibiotic treatment of osteomyelitis. The use of lactic acid oligomer as a carrier.
• Alveolar bone regeneration using absorbable poly(L-lactide-co-varepsilon-caprolactone)/beta-tricalcium phosphate membrane and gelatin sponge incorporating basic fibroblast growth factor.
• An immuno-isolative membrane capable of consuming cytolytic complement proteins.
• Bone formation at a rabbit skull defect by autologous bone marrow cells combined with gelatin microspheres containing TGF-beta1.
• Controlled release of vascular endothelial growth factor by use of collagen hydrogels.
• Cross-linking of amniotic membranes.
• Depressed tuberculin sensitivity in Darier's disease.
• Depressed tuberculin sensitivity in porokeratosis.
• Dextran sulfate as a material for the preparation of a membrane for immunoisolation.
• Ectopic bone formation induced by biodegradable hydrogels incorporating bone morphogenetic protein.
• Encapsulation of mammalian cells into synthetic polymer membranes using least toxic solvents.
• Enzyme-catalyzed synthesis of a bioactive oligopeptide in nearly anhydrous solvents with polyethylene glycol-modified proteases.
• Fusogenic activity of various water-soluble polymers.
• In vivo chondrogenesis in collagen sponge sandwiched by perichondrium.
• Influence of gelatin complexation on cell proliferation activity and proteolytic resistance of basic fibroblast growth factor.
• Interaction of poly(2-acrylamido 2-methylpropane sulfonate)-grafted polystyrene beads with cationic complement proteins.
• Interaction of poly(styrene sulfonic acid) with the alternative pathway of the serum complement system.
• Interaction of poly(styrene sulfonic acid) with the classical pathway of the serum complement system.
• Letter: Hyperkeratosis lenticularis perstans.
• Neovascularization effect of biodegradable gelatin microspheres incorporating basic fibroblast growth factor.
• New biodegradable oligoesters for pharmaceutical application.
• New detachable balloon technique for traumatic carotid-cavernous sinus fistulae.
• Preparation of DNA-immobilized immunoadsorbent for treatment of systemic lupus erythematosus.
• Regeneration of extrahepatic bile duct -possibility to clinical application by recognition of the regenerative process.
• Regeneration of the inferior vena cava with a bioabsorbable polymer implant: a histological study.
• Soft tissue adhesive composed of modified gelatin and polysaccharides.
• Spatial gradient effects of 120 mT static magnetic field on endothelial tubular formation in vitro.
• Surface modification of silicone for percutaneous implantation.
• Treatment of a spontaneous carotid cavernous fistula using an electrodetachable microcoil.
• Vascularization into a porous sponge by sustained release of basic fibroblast growth factor.