[Influence of cryoprotectant and cooling rate in vitrification method on the spindles of rabbit oocytes.]

[Influence of cryoprotectant and cooling rate in vitrification method on the spindles of rabbit oocytes.] Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

[Influence of cryoprotectant and cooling rate in vitrification method on the spindles of rabbit oocytes.] Abstract Text:

Xue-yong Cai,Gui-an Chen,Ying Lian,Xiao-ying Zheng,Hong-mei Peng,

OBJECTIVE: To investigate the influence of cryoprotectants and cooling rates in vitrification method on the spindles of rabbit M II oocytes. METHODS: Rabbit oocytes were verified by using cryoloop with ethylene glycol (EG) singly or EG combined with dimethyl sulphoxide (DMSO) as cryoprotectants, and cooled by taking oocytes directly into liquid nitrogen or by vitrification machine. After frozen rabbit oocytes thawed, the microtubulin and chromosome of the spindles were fixation and stained by immunofluorescent method. Confocal microscope was used to reveal spindle configuration. RESULTS: In the two protocols of single EG used and EG combined with DMSO, the spindles were severely injured. But in protocol of EG combined with DMSO and at ultra-rapid cooling rate, the normal configuration of spindle rate of thawed rabbit oocytes was similar to that of the control group. CONCLUSION: The protocol of EG combined with DMSO as cryoprotectants and with extremely high cooling rate by vitrification machine can produce the best effect on conservation of spindle configuration in vitrification of rabbit oocytes.

[Influence of cryoprotectant and cooling rate in vitrification method on the spindles of rabbit oocytes.] Publishing Authors By Initials

XY Cai,GA Chen,Y Lian,XY Zheng,HM Peng,

For similar abstracts research abstracts see: abstracts research

PUBMED ID PMID:

MEDLINE DATE:

[Influence of cryoprotectant and cooling rate in vitrification method on the spindles of rabbit oocytes.] Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Beijing da xue xue bao. Yi xue ban = Journal of Pe

VOLUME: 36

Page Numbers: 566-70

Journal Abbreviation: Beijing Da Xue Xue Bao

ISSN: 1671-167X

DAY: 17

MONTH: Dec

YEAR: 2004

[Influence of cryoprotectant and cooling rate in vitrification method on the spindles of rabbit oocytes.] Information

Number of References:

LANGUAGE: chi

NlmUniqueID: 101125284

[Influence of cryoprotectant and cooling rate in vitrification method on the spindles of rabbit oocytes.] Keywords Mesh Terms:

KEYWORDS:

MESH TERMS:

Chemical & Substance for Abstract: [Influence of cryoprotectant and cooling rate in vitrification method on the spindles of rabbit oocytes.] Information

Substance Name:

Registry Number:

Grant and Affiliation Information for [Influence of cryoprotectant and cooling rate in vitrification method on the spindles of rabbit oocytes.]

AFFILIATION: Department of Obstetrics and Gynecology, Peking University Third Hospital, Beijing 100083, China.

Country: China

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: Beijing Da Xue Xue Bao

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Influence of cryoprotectant and cooling rate in vitrification method on the spindles of rabbit oocytes Related Publications

• Complications in children with long-term cochlear implants.
• Glycerate-oxidizing activity of glycolate oxidase from leaves of Spinacia oleracea.
• Highly luminescent, stable, and water-soluble CdSe/CdS core-shell dendron nanocrystals with carboxylate anchoring groups.
• Treatment of Meniere's disease with a heavy dosage of gu sui bu (Rhizoma Drynariae).
• [A two-dimensional electrophoresis protocol suitable for proteomic study of rice leaves]
• [Comparative studies on the difference between Ascaris lumbricoides and Ascaris suum]
• [Detection of the optimum resolution of monochromator]
• [Determination of Cu, Pb, Sn, Fe and Zn in the ancient Chinese bronzes by X-ray fluorescence filter paper method]
• [Determination of rhodojaponin, a novel botanical insecticide by HPTLC]
• [Determination of trace rare earth impurities in the high-purity lanthanum oxide by chemical spectrographic analysis]
• [Diagnosis and treatment of gluteal muscle contracture associated with unequal leg length caused by pelvis obliquity]
• [Effect of Sim2 gene on the differentiation of PC12 cells]
• [Effect of soy isoflavone on gene expression of leptin and insulin sensibility in insulin-resistant rats]
• [Fabrication of a blood vessel scaffold with a combined polymer for tissue engineering]
• [Improving the management of deep partial thickness burn wound]
• [Influence of angiotensin-converting enzyme gene polymorphism on the anti-proteinuria efficacy of angiotensin-converting enzyme inhibitor in Han nationality of southern Sichuan province in China]
• [Influence of cryoprotectant and cooling rate in vitrification method on the spindles of rabbit oocytes.]
• [Investigation on hepatitis G virus (HGV) infection among different populations in Shenzhen]
• [Long-term expansion of T cell progenitors by JAK3 gene transduced primitive hematopoietic cells in vitro]
• [Retroviral-mediated transfection of hepatitis B virus core gene into bone marrow-derived dendritic cells]
• [Spectroscopic studies on transition metal ions in colored diamonds]
• [Study of the clinical factors associated with glomerular filtration rate]
• [Study on the anti-maturation features of immature dendritic cells induced by low dose of granulocyte macrophage colony stimulating factor]
• [Successful emergency treatment of a case with severe hemolytic disease of the newborn due to maternal Rh deletion phenotype-D]
• [Targeting of nitric oxide-donor and related drugs]
• [The hardware design of a portable gastrointestinal wireless endoscope image receiver]
• [The influence of continuous renal replacement therapy on the plasma levels of endotoxin and cytokines in severely burned patients with sepsis]
• [The influence of temperature on the protein expression of human lung cancer cell line A549]
• [The oxidation degradation of great green SF by Fenton reagent]
• [The research advance of analytical methodology for detecting bovine spongiform encephalopathy]