Influence of Autoinducer-2 (AI-2) and Beef Sample Extracts on E. coli O157:H7 Survival and Gene Expression of Virulence Genes yadK and hhA.

Influence of Autoinducer-2 (AI-2) and Beef Sample Extracts on E. coli O157:H7 Survival and Gene Expression of Virulence Genes yadK and hhA. Research Abstract Details 

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Influence of Autoinducer-2 (AI-2) and Beef Sample Extracts on E. coli O157:H7 Survival and Gene Expression of Virulence Genes yadK and hhA. Abstract Text:

K A Soni,L Lu,P R Jesudhasan,M E Hume,S D Pillai,K A Soni,L Lu,P R Jesudhasan,M E Hume,S D Pillai,

Bacterial cell-to-cell communication is mediated by autoinducer (AI) molecules such as AI-2 and has been reported to regulate gene expression in Escherichia coli O157:H7. We have previously shown that ground beef contains compounds that can inhibit sensing of AI-2 like activity. The hypothesis of this study was that AI-2 activity observed in conditioned medium (CM) will enhance E. coli O157:H7 survival and expression of virulence genes, whereas compounds inhibitory (such as those present in ground beef extracts) to AI-2 activity will negate these effects. E. coli O157:H7 luxS mutant strain VS 94 (incapable of synthesizing AI-2) was employed in these studies. The survival of this enteric bacterial pathogen as a function of AI-2 activity and the presence of AI-2 inhibitory compounds was studied at 4 degrees C. The number of survivors in the presence of AI-2 was significantly higher compared to the absence of AI-2, and the addition of ground beef extracts to conditioned medium negated the influence of AI-2 activity. Autoinducer AI-2 upregulated selected genes virulence genes (yadK, and hha), whereas the ground beef extract reversed the effect of AI-2 on the expression of the selected genes.

Influence of Autoinducer-2 (AI-2) and Beef Sample Extracts on E. coli O157:H7 Survival and Gene Expression of Virulence Genes yadK and hhA. Publishing Authors By Initials

KA Soni,L Lu,PR Jesudhasan,ME Hume,SD Pillai,KA Soni,L Lu,PR Jesudhasan,ME Hume,SD Pillai,

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Influence of Autoinducer-2 (AI-2) and Beef Sample Extracts on E. coli O157:H7 Survival and Gene Expression of Virulence Genes yadK and hhA. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Journal of food science

VOLUME: 73

Page Numbers: M135-9

Journal Abbreviation: J. Food Sci.

ISSN: 1750-3841

DAY: 4

MONTH: Apr

YEAR: 2008

Influence of Autoinducer-2 (AI-2) and Beef Sample Extracts on E. coli O157:H7 Survival and Gene Expression of Virulence Genes yadK and hhA. Information

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LANGUAGE: eng

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AFFILIATION: Dept. of Poultry Science & Nutrition and Food Science, Texas A & M Univ., College Station, TX 77843, U.S.A.

Country: United States

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MEDLINETA: J Food Sci

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