Induction of TNF-alpha by LPS in Schwann Cell is Regulated by MAPK Activation Signals.

Induction of TNF-alpha by LPS in Schwann Cell is Regulated by MAPK Activation Signals. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Induction of TNF-alpha by LPS in Schwann Cell is Regulated by MAPK Activation Signals. Abstract Text:

Chun Cheng,Yongwei Qin,Xiaoyi Shao,Haibo Wang,Yongjing Gao,Mengling Cheng,Aiguo Shen,Chun Cheng,Yongwei Qin,Xiaoyi Shao,Haibo Wang,Yongjing Gao,Mengling Cheng,Aiguo Shen,

Mitogen-activated protein kinases (MAPKs) are important mediators of cytokine expression and are critically involved in the immune response. The lipopolysaccharide (LPS) of gram-negative bacteria induces the expression of cytokines and proinflammatory genes via the toll-like receptor 4 (TLR4) signaling pathway in diverse cell types. In vivo, Schwann cells (SCs) at the site of injury may also produce tumor necrosis factor-- alpha (TNF-alpha). However, the precise mechanisms of TNF-alpha synthesis are still not clear. The purpose of the present study was to elucidate the underlying molecular mechanisms in the cultured SCs for its ability to activate the MAPKs and TNF-alpha gene, in response to LPS. Using enzyme-linked immunosorbent assay (ELISA), it was confirmed that treatment with LPS stimulated the synthesis of TNF-alpha in a concentration- and time-dependent manner. Intracellular location of TNF-alpha was detected under confocal microscope. Moreover, LPS activated extracellular signal-regulated kinase (ERK1/2), P38 and stress activated protein kinase/c-Jun N-terminal kinase (SAPK/JNK) and induced their phosphorylation. LPS-elicited SCs TNF-alpha production was also drastically suppressed by PD98059 (ERK inhibitor), SB202190 (P38 inhibitor), or SP600125 (SAPK/JNK inhibitor). Additionally, the expression of CD14 and TLR4 was examined by RT-PCR. It was demonstrated that the expression of CD14, TLR4 was crucial for the SCs responses to LPS. In conclusion, the results provide novel mechanisms for the response of SCs to LPS stimulation, through MAPKs signaling pathways.

Induction of TNF-alpha by LPS in Schwann Cell is Regulated by MAPK Activation Signals. Publishing Authors By Initials

C Cheng,Y Qin,X Shao,H Wang,Y Gao,M Cheng,A Shen,C Cheng,Y Qin,X Shao,H Wang,Y Gao,M Cheng,A Shen,

For similar abstracts research abstracts see: abstracts research

PUBMED ID PMID:

MEDLINE DATE:

Induction of TNF-alpha by LPS in Schwann Cell is Regulated by MAPK Activation Signals. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Cellular and molecular neurobiology

VOLUME: 27

Page Numbers: 909-21

Journal Abbreviation: Cell. Mol. Neurobiol.

ISSN: 0272-4340

DAY: 28

MONTH: 09

YEAR: 2007

Induction of TNF-alpha by LPS in Schwann Cell is Regulated by MAPK Activation Signals. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 8200709

Induction of TNF-alpha by LPS in Schwann Cell is Regulated by MAPK Activation Signals. Keywords Mesh Terms:

KEYWORDS:

MESH TERMS:

Chemical & Substance for Abstract: Induction of TNF-alpha by LPS in Schwann Cell is Regulated by MAPK Activation Signals. Information

Substance Name:

Registry Number:

Grant and Affiliation Information for Induction of TNF-alpha by LPS in Schwann Cell is Regulated by MAPK Activation Signals.

AFFILIATION: Key Laboratory for Neuroregeneration of JiangSu Province, Nantong University, 19 Qixiu Road, Nantong, 226001, Jiangsu, PR China, shen_aiguo@yahoo.com.

Country: United States

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: Cell Mol Neurobiol

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Induction of TNF-alpha by LPS in Schwann Cell is Regulated by MAPK Activation Signals Related Publications

• Comment on "Direct measurement of the 'Giant' adiabatic temperature change in Gd5Si2Ge2".
• Comparison of IL-6-induced STAT1 activation in different responsive cells.
• [Advanced life support in prehospital emergency treatment of trauma]
• [Application of long distance-PCR to hemophilia A carrier detection and prenatal diagnosis]
• [Cell cycle analysis and P53 gene mutation detection of fibroblasts derived from the surrounding skin of keloids]
• [Clinical investigation of application of lung biopsy in the diagnosis of diffuse pulmonary interstitial disease]
• [Continuous target of China's manned space project and research direction of space medico-engineering]
• [Coronary artery bypass grafting after acute myocardial infarction]
• [Development of an anti-rTNF-alpha chimeric antibody]
• [Effect of staining method and sintering temperature on the color of porcelain-fused-to-metal restorations]
• [Effects of microgravity on human cognitive function in space flight]
• [Epidemic investigation on 3 to 6 years children's bruxism in Shanghai.]
• [Epidemiology and drug resistance of the pathogenic microbes in the complicated infection of hematological malignancies]
• [European Society of cardiology guideline (III): percutaneous coronary interventions Emergency department]
• [Expectation of the study of weightlessness physiology in the 21st century]
• [Expression of adhesion molecules on CD34(+) hematopoietic precursor cells from normal human bone marrow, cord blood and mobilized blood]
• [Expression of monoclonal nonspecific suppressor factor beta in E. coli, preparation of its antibodies and its tissue orientation in the mouse endometrium]
• [Fiberoptic ductoscopy for patients with intraductal papillary lesions]
• [Non-extraction approach using RPE to correct Angle Class I malocclusion with moderate crowding: A cephalometric study]
• [Observation on the effects of urapidil on hemodynamics in heart dysfunction dogs by thoracic electrical bioimpedance]
• [Relationship between the human zygote type and embryo implantation potential]
• [Relationship of vitamin D receptor polymorphism with breast cancer]
• [Science face upto to fact of undiscovered, frustration and challenge from severe acute respiratory syndrome]
• [Site-specific mutagenesis of murine anti-human TNF-alpha Fab gene and its expression in E.coli]
• [Study on the mechanism of the effects of recombinant rat tumor necrosis factor alpha on the degradation of rat skeletal muscle proteins]
• [Study on the relationship between sulfonylurea receptor 1 gene polymorphism and type 2 diabetes mellitus]
• [Superiority of traditional Chinese medicine on gene regulation]
• [The epidemiological study of severe acute respiratory syndrome]
• [Transfer and expression of rh-SCF gene in human hematopoietic stem/progenitor cells]
• [Treatment of AMI with ST segment hoist]