Induction of ornithine decarboxylase in cultured mouse L cells. I. Effects of cellular growth, hormones, and actinomycin D.

Induction of ornithine decarboxylase in cultured mouse L cells. I. Effects of cellular growth, hormones, and actinomycin D. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Induction of ornithine decarboxylase in cultured mouse L cells. I. Effects of cellular growth, hormones, and actinomycin D. Abstract Text:

Y Yamasaki,A Ichihara,

The activity of ornithine decarboxylase [EC 4.1.1.17] (ODC) in mouse L cells in the confluent state was induced within 4 hr by cyclic AMP (cAMP) or by insulin. During growth of L cells the concentration of cAMP increased first, then induction of ODC occurred and finally the cell number increased: the levels of cAMP and ODC increased only transitorily and returned to the basal levels when the cells become confluent. In growing cultures, however, the presence of cAMP reduced induction of ODC and cell growth. These results suggest that cAMP is involved in induction of ODC and that its concentration may be important for enzyme induction as well as for cell growth. Actinomycin D with or without these inducers stimulated induction of ODC in L cells, whereas cycloheximide inhibited it, suggesting that these hormones affect the translational level of ODC synthesis. The effect of actinomycin D on induction of ODC was much greater in non-growing cells than in growing cells. It was also found that the half life of ODC was 81 min in non-growing cells and 112 min in growing cells. This suggests that turnover of the enzyme is more rapid in the non-growing than in the growing state and that there may be an RNA fraction which controls its turnover and which also has a very short half life.

Induction of ornithine decarboxylase in cultured mouse L cells. I. Effects of cellular growth, hormones, and actinomycin D. Publishing Authors By Initials

Y Yamasaki,A Ichihara,

For similar enzymes and coenzymes: enzymes: lyases: carbon-carbon lyases: carboxy-lyases: ornithine decarboxylase research abstracts see: enzymes and coenzymes: enzymes: lyases: carbon-carbon lyases: carboxy-lyases: ornithine decarboxylase research

PUBMED ID PMID:

MEDLINE DATE:

Induction of ornithine decarboxylase in cultured mouse L cells. I. Effects of cellular growth, hormones, and actinomycin D. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Journal of biochemistry

VOLUME: 80

Page Numbers: 557-62

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: Sep

YEAR: 1976

Induction of ornithine decarboxylase in cultured mouse L cells. I. Effects of cellular growth, hormones, and actinomycin D. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 376600

Induction of ornithine decarboxylase in cultured mouse L cells. I. Effects of cellular growth, hormones, and actinomycin D. Keywords Mesh Terms:

KEYWORDS: Ornithine Decarboxylase

MESH TERMS: biosynthesis

Chemical & Substance for Abstract: Induction of ornithine decarboxylase in cultured mouse L cells. I. Effects of cellular growth, hormones, and actinomycin D. Information

Substance Name: Ornithine Decarboxylase

Registry Number: EC 4.1.1.17

Grant and Affiliation Information for Induction of ornithine decarboxylase in cultured mouse L cells. I. Effects of cellular growth, hormones, and actinomycin D.

AFFILIATION:

Country: JAPAN

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: J Biochem

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Induction of ornithine decarboxylase in cultured mouse L cells I Effects of cellular growth, hormones, and actinomycin D Related Publications

• Biochemical studies on liver functions in primary cultured hepatocytes of adult rats. I. Hormonal effects on cell viability and protein synthesis.
• Biochemical studies on liver functions in primary cultured hepatocytes of adult rats. II. Regulation of protein and amino acid metabolism.
• Biochemical studies on liver functions in primary cultured hepatocytes of adult rats. III. Changes of enzyme activities on cell membranes during culture.
• Biosynthesis of castor oil: effect of polyamines on the acylation of lysophosphatidic acid at the sn-2 position with ricinoleic acid.
• Control of ketogenesis from amino acids. II. Ketone bodies formation from alpha-ketoisocaproate, the keto-analogue of leucine, by rat liver mitochondria.
• Control of ketogenesis from amino acids. IV. Tissue specificity in oxidation of leucine, tyrosine, and lysine.
• Density-dependent growth control of adult rat hepatocytes in primary culture.
• Different effect of amino acid deprivation on syntheses of intra- and extracellular proteins in rat hepatocytes in primary culture.
• Globin s-propyl cysteine and urinary N-acetyl-S-propylcysteine as internal biomarkers of 1-bromopropane exposure.
• Hormonal control of serine dehydratase mRNA in primary cultures of adult rat hepatocytes.
• Hormonal regulation of translatable mRNA of tryptophan 2,3-dioxygenase in primary cultures of adult rat hepatocytes.
• Hormonal regulations of glucose-6-phosphate dehydrogenase and lipogenesis in primary cultures of rat hepatocytes.
• Identification of trypsin inhibitor in bovine pituitary extracts as a survival factor for adult rat hepatocytes in primary culture.
• Induction of ornithine decarboxylase in cultured mouse L cells. I. Effects of cellular growth, hormones, and actinomycin D.
• Induction of ornithine decarboxylase in cultured mouse L cells. II. Effects of additions of amino acids and serum.
• Inhibition of protein synthesis in mouse L cells by poly-L-ornithine.
• Regulatory relation between insulin receptor and its functional responses in primary cultured hepatocytes of adult rats.
• Requirement of prolonged presence of a high intracellular level of cyclic AMP for induction of serine dehydratase in primary cultured rat hepatocytes.
• Reversibility of the adverse effects of 1-bromopropane exposure in rats.
• Role of serum in maintenance of functional hepatocytes in primary culture.
• Structure of SixA, a Histidine Protein Phosphatase of the ArcB Histidine-Containing Phosphotransfer Domain in Escherichia coli.
• The ammonia-free partial reduction of substituted pyridinium salts.
• The selective effect of the image of a hand on visuotactile interactions as assessed by performance on the crossmodal congruency task.
• Transaminase of branched chain amino acids. 3. Purification and properties of the mitochondrial enzyme from hog heart and comparison with the supernatant enzyme.
• Transaminase of branched chain amino acids. 8. Further studies on regulation of isozyme activities in rat liver and kidney.
• Transaminase of branched chain amino acids. IX. Conformational change of isozyme I in vitro.
• Transaminase of branched chain amino acids. VI. Purification and properties of the hog brain enzyme.
• Transaminase of branched chain amino acids. VII. Comparative studies on isozymes of ascites hepatoma and various normal tissues of rat.
• Transfer of purines from liver to erythrocytes. In vivo and in vitro studies.
• Unique heme environment at the putative distal region of hydrogen peroxide-dependent fatty acid alpha-hydroxylase from Sphingomonas paucimobilis (peroxygenase P450(SPalpha).