Induction of NKG2D ligands on human dendritic cells by TLR ligand stimulation and RNA virus infection.

Induction of NKG2D ligands on human dendritic cells by TLR ligand stimulation and RNA virus infection. Research Abstract Details 

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Induction of NKG2D ligands on human dendritic cells by TLR ligand stimulation and RNA virus infection. Abstract Text:

Takashi Ebihara,Hisayo Masuda,Takashi Akazawa,Masashi Shingai,Hideaki Kikuta,Tadashi Ariga,Misako Matsumoto,Tsukasa Seya,Takashi Ebihara,Hisayo Masuda,Takashi Akazawa,Masashi Shingai,Hideaki Kikuta,Tadashi Ariga,Misako Matsumoto,Tsukasa Seya,

Monocyte-derived dendritic cells (mDCs) and NK cells are reciprocally activate via cytokines and cell-cell contact. Although seven human NKG2D ligands (NKG2DLs), UL16-binding proteins (ULBP) 1, 2, 3 and 4, retinoic acid early transcript 1G (RAET1G) and MHC class I-related chains A and B, have been reported, the differential distribution and roles of these ligands in the maturation of human mDCs have not been elucidated. In the present study, we produced polyclonal antibodies (pAbs) directed against human ULBP1, 2 and 3. All these ULBPs were detected on human mDCs when probed by the pAbs, although their expression profiles were different. We next investigated what kinds of Toll-like receptor agonists and RNA viruses [influenza virus, human respiratory syncytial virus (RSV), measles virus and hepatitis C virus (HCV)] induced the expression of NKG2DLs on mDCs. ULBP1 was up-regulated on mDCs in response to LPS or infection with RSV. The expression of ULBP2 was induced by LPS and poly I:C, indicating that the TIR-containing adapter molecule-1 (TIR domain-containing adaptor-inducing IFN) pathway is associated with ULBP2 induction. Although infection with HCV did not cause up-regulation of NKG2DLs, other RNA virus infections and poly I:C promoted expression of ULBP2 and RAET1G in an IFN-alpha/beta-independent manner. Finally, the over-expression of ULBP1 and 2 on mDCs facilitated NK cell proliferation and IFN-gamma production through a mDC-NK cell interaction in the presence of IL-2. Hence, the results reflect the important role of NKG2DLs on human mDCs in mDC-mediated NK cell activation.

Induction of NKG2D ligands on human dendritic cells by TLR ligand stimulation and RNA virus infection. Publishing Authors By Initials

T Ebihara,H Masuda,T Akazawa,M Shingai,H Kikuta,T Ariga,M Matsumoto,T Seya,T Ebihara,H Masuda,T Akazawa,M Shingai,H Kikuta,T Ariga,M Matsumoto,T Seya,

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Induction of NKG2D ligands on human dendritic cells by TLR ligand stimulation and RNA virus infection. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: International immunology

VOLUME: 19

Page Numbers: 1145-55

Journal Abbreviation: Int. Immunol.

ISSN: 0953-8178

DAY: 18

MONTH: 09

YEAR: 2007

Induction of NKG2D ligands on human dendritic cells by TLR ligand stimulation and RNA virus infection. Information

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LANGUAGE: eng

NlmUniqueID: 8916182

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Grant and Affiliation Information for Induction of NKG2D ligands on human dendritic cells by TLR ligand stimulation and RNA virus infection.

AFFILIATION: Department of Microbiology and Immunology, Hokkaido University Graduate School of Medicine, Kita-15, Nishi-7, Kita-ku, Sapporo 060-8638, Japan.

Country: England

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MEDLINETA: Int Immunol

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