Induction of interleukin-12 by lactobacillus strains having a rigid cell wall resistant to intracellular digestion.

Induction of interleukin-12 by lactobacillus strains having a rigid cell wall resistant to intracellular digestion. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Induction of interleukin-12 by lactobacillus strains having a rigid cell wall resistant to intracellular digestion. Abstract Text:

K Shida,J Kiyoshima-Shibata,M Nagaoka,K Watanabe,M Nanno,

Some strains of lactobacilli can stimulate macrophages and dendritic cells to secrete IL-12, which plays a key role in activating innate immunity. We examined the IL-12-inducing ability of 47 Lactobacillus strains belonging to 10 species in mouse peritoneal macrophages, and characterized the properties important for the induction of IL-12. Although considerable differences in IL-12-inducing ability were observed among the strains tested, almost all strains belonging to the Lactobacillus casei group (L. casei, Lactobacillus rhamnosus, and Lactobacillus zeae) or to Lactobacillus fermentum induced high levels of IL-12. Phagocytosis of lactobacilli was necessary for IL-12 induction, and the strains with strong IL-12 induction were relatively resistant to lysis in the macrophages. The sensitivity of Lactobacillus strains to in vitro treatment with M-1 enzyme, a member of the N-acetylmuramidases, was negatively correlated with IL-12-inducing ability. Using a probiotic strain, L. casei strain Shirota (LcS), we showed that the cell wall of LcS could be digested by long-term treatment with a high dose of M-1 enzyme and that the IL-12-inducing ability was diminished according to the duration of the enzyme treatment. The soluble polysaccharide-peptidoglycan complex released from the cell wall of LcS did not induce IL-12, whereas the insoluble intact cell wall of LcS induced IL-12. These results suggest that the intact cell wall structure of lactobacilli is an important element in the ability to induce IL-12 and that Lactobacillus strains having a rigid cell wall resistant to intracellular digestion effectively stimulate macrophages to induce IL-12.

Induction of interleukin-12 by lactobacillus strains having a rigid cell wall resistant to intracellular digestion. Publishing Authors By Initials

K Shida,J Kiyoshima-Shibata,M Nagaoka,K Watanabe,M Nanno,

For similar abstracts research abstracts see: abstracts research

PUBMED ID PMID:

MEDLINE DATE:

Induction of interleukin-12 by lactobacillus strains having a rigid cell wall resistant to intracellular digestion. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Journal of dairy science

VOLUME: 89

Page Numbers: 3306-17

Journal Abbreviation:

ISSN: 1525-3198

DAY: 10

MONTH: Sep

YEAR: 2006

Induction of interleukin-12 by lactobacillus strains having a rigid cell wall resistant to intracellular digestion. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 2985126

Induction of interleukin-12 by lactobacillus strains having a rigid cell wall resistant to intracellular digestion. Keywords Mesh Terms:

KEYWORDS:

MESH TERMS:

Chemical & Substance for Abstract: Induction of interleukin-12 by lactobacillus strains having a rigid cell wall resistant to intracellular digestion. Information

Substance Name:

Registry Number:

Grant and Affiliation Information for Induction of interleukin-12 by lactobacillus strains having a rigid cell wall resistant to intracellular digestion.

AFFILIATION: Yakult Central Institute for Microbiological Research, 1796 Yaho, Kunitachi, Tokyo 186-8650, Japan. kan-shida@yakult.co.jp

Country: United States

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: J Dairy Sci

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Induction of interleukin-12 by lactobacillus strains having a rigid cell wall resistant to intracellular digestion Related Publications

• Induction of interleukin-12 by lactobacillus strains having a rigid cell wall resistant to intracellular digestion.