Induction of hepatic haematopoiesis with fibronectin expression by EMT stromal cells during the second trimester of development.

Induction of hepatic haematopoiesis with fibronectin expression by EMT stromal cells during the second trimester of development. Research Abstract Details 

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Induction of hepatic haematopoiesis with fibronectin expression by EMT stromal cells during the second trimester of development. Abstract Text:

M Lambropoulou,D Tamiolakis,I Venizelos,G Alexiadis,G Anastasopoulos,V Limberis,G Galazios,P Tsikouras,M Simopoulou,S Nikolaidou,G Petrakis,N Papadopoulos,M Lambropoulou,D Tamiolakis,I Venizelos,G Alexiadis,G Anastasopoulos,V Limberis,G Galazios,P Tsikouras,M Simopoulou,S Nikolaidou,G Petrakis,N Papadopoulos,M Lambropoulou,D Tamiolakis,I Venizelos,G Alexiadis,G Anastasopoulos,V Limberis,G Galazios,P Tsikouras,M Simopoulou,S Nikolaidou,G Petrakis,N Papadopoulos,

In an initial period of vertebrate phylogeny (bone marrow-less vertebrates), lymphohaematopoiesis takes place in numerous organs containing a suitable microenvironment. Among other organs (i.e., gonads, kidney and spleen), the liver is apparently the most appropriate site for homing and differentiation of haematopoietic cell precursors. Interaction between haematopoietic cells and stromal cells is important for regulation of haematopoiesis. Numerous soluble and membrane-bound factors directly regulating haematopoiesis have been documented, but little is known about the effect of the foetal hepatic epithelial-to-mesenchymal transition (EMT) stromal cells' activity and their product-fibronectin, on foetal hepatic haematopoiesis. The binding of late-stage erythroid cells to FN has been well characterised and is believed to be critical for the terminal stages of erythroid differentiation. The intention of this article is to provide a quantitative overview of FN, produced by hepatic EMT stromal cells, in foetal hepatic haematopoiesis during the first and second trimester of development. Paraffin-embedded specimens from the liver of 30 human embryos in the first and second trimesters of gestation were investigated by conventional histology and immunohistology for the presence of FN and specific haematopoietic cell types. The staining intensity, and localisation of FN and haematopoietic markers in sequential sections were examined. Furthermore, double immunohistochemical staining was performed to assess simultaneous detection of FN and haematopoietic markers. FN was expressed in the EMT stromal cells of the hepatic portal triads more strongly during the second trimester than the first. Furthermore, an intense immunostaining for haematopoietic lineages, and especially for erythropoiesis, was observed in the second trimester compared to the first. The results of the double immunostaining disclosed an intimate co-expression of the FN and CD haematopoietic markers. Foetal hepatic EMT stromal cells provide a unique microenvironment that supports the emergence, expansion and maintenance of human foetal haematopoietic development during the mid-gestational stage. FN produced by the EMT stromal cells follows a time course parallel to that of haematopoiesis. We suggest that in foetal liver, phenotypic modifications of EMT stromal cells expressing FN concerning the cell adhesion capacity of the protein are associated with proliferation and differentiation of specific haematopoietic cell lineages during the second trimester of gestation, probably reflecting the increasing demand of the growing foetus for mature erythroid and myeloid cells.

Induction of hepatic haematopoiesis with fibronectin expression by EMT stromal cells during the second trimester of development. Publishing Authors By Initials

M Lambropoulou,D Tamiolakis,I Venizelos,G Alexiadis,G Anastasopoulos,V Limberis,G Galazios,P Tsikouras,M Simopoulou,S Nikolaidou,G Petrakis,N Papadopoulos,M Lambropoulou,D Tamiolakis,I Venizelos,G Alexiadis,G Anastasopoulos,V Limberis,G Galazios,P Tsikouras,M Simopoulou,S Nikolaidou,G Petrakis,N Papadopoulos,M Lambropoulou,D Tamiolakis,I Venizelos,G Alexiadis,G Anastasopoulos,V Limberis,G Galazios,P Tsikouras,M Simopoulou,S Nikolaidou,G Petrakis,N Papadopoulos,

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Induction of hepatic haematopoiesis with fibronectin expression by EMT stromal cells during the second trimester of development. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Clinical and experimental medicine

VOLUME: 7

Page Numbers: 115-21

Journal Abbreviation: Clin. Exp. Med.

ISSN: 1591-8890

DAY: 3

MONTH: 10

YEAR: 2007

Induction of hepatic haematopoiesis with fibronectin expression by EMT stromal cells during the second trimester of development. Information

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LANGUAGE: eng

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AFFILIATION: Department of Histology/Embryology, Democritus University of Thrace, Dragana, 68 100 Alexandroupolis, Greece.

Country: Italy

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MEDLINETA: Clin Exp Med

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