Induction of estrus and superovulation in seasonally anestrous ewes.

Induction of estrus and superovulation in seasonally anestrous ewes. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Induction of estrus and superovulation in seasonally anestrous ewes. Abstract Text:

K R Bondioli,R L Allen,R W Wright,

The induction of estrus in 17 previously cycling nulliparous ewes, 9 to 10 months of age, was attempted with Medroxyprogesterone acetate (MAP) pessaries during the early anestrous period (March-April). Ewes were verified to be anestrous by the lack of estrous behavior in the presence of a vasectomized ram and by a radioimmunoassay for serum progesterone in two samples taken 7 days apart showing less than 1 ng/ml serum progesterone. Superovulation was attempted with injections of either FSH or FSH + LH. MAP vaginal pessaries remained in place for a period of 12 days and FSH was administered to all ewes (IM) at 12 hr intervals over a 3 day period; 5 mg was injected twice on day 11 after pessary insertion, followed by 4 and 3 mg injections twice daily on each succeeding day, for a total of 24 mg per ewe. Nine ewes were given 25 mg LH (IV) within 8 hrs after the onset of behavioral estrus in addition to FSH. Ewes were hand-mated to several rams at 12 hr intervals throughout the estrus period. Ovulation and fertilization rates were recorded for each ewe following midline laparotomy and embryo collection. All ewes were in estrus between 36 and 48 hrs after removal of the MAP pessaries. In ewes injected with FSH only, 8 of 8 ovulated with a mean ovulation rate of 6.0 +/- 4.4 and a fertilization rate of 70%. Nine of 9 ewes receiving both FSH + LH ovulated with a mean ovulation rate of 13.9 +/- 13.1 and a fertilization rate of 72%. Statistical analysis by Students t-test resulted in differences in number of ova recovered (P<.05) between FSH only and FSH + LH treated ewes and a trend towards increased ovulation rate in FSH + LH treated ewes. These results show that early seasonally anestrous ewes can be successfully induced and synchronized for estrus with MAP pessaries and the number of ova recovered is increased with the inclusion of LH in the superovulation regime.

Induction of estrus and superovulation in seasonally anestrous ewes. Publishing Authors By Initials

KR Bondioli,RL Allen,RW Wright,

For similar abstracts research abstracts see: abstracts research

PUBMED ID PMID:

MEDLINE DATE:

Induction of estrus and superovulation in seasonally anestrous ewes. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Theriogenology

VOLUME: 18

Page Numbers: 209-14

Journal Abbreviation: Theriogenology

ISSN: 0093-691X

DAY: 26

MONTH: Aug

YEAR: 1982

Induction of estrus and superovulation in seasonally anestrous ewes. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 421510

Induction of estrus and superovulation in seasonally anestrous ewes. Keywords Mesh Terms:

KEYWORDS:

MESH TERMS:

Chemical & Substance for Abstract: Induction of estrus and superovulation in seasonally anestrous ewes. Information

Substance Name:

Registry Number:

Grant and Affiliation Information for Induction of estrus and superovulation in seasonally anestrous ewes.

AFFILIATION: Washington State University,Pullman, WA 99164 USA.

Country: United States

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: Theriogenology

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Induction of estrus and superovulation in seasonally anestrous ewes Related Publications

• A review of the epidemiologic aspects of embryo transfer from Brucella abortus -infected cows.
• Age and gender differences and predictors of victimization of the older homeless.
• Bovine amniotic and allantoic fluids for the culture of murine embryos.
• Bovine embryo morphology and evaluation.
• Coculture of bovine embryos with bovine fibroblasts.
• Cold culture of different stage mouse embryos in bicarbonated and bicarbonate-free media.
• Cold storage of mouse embryos of different stages of development.
• Deep freezing of mouse one-cell embryos and oocytes using different cryoprotectants.
• Determination of pentose phosphate and Embden-Meyerhof pathway activities in bovine embryos.
• Development of one-cell ovine embryos in two culture media under two gas atmospheres.
• Development of one-cell porcine embryos in two culture systems.
• Effect of one-step sucrose dilution of glycerol on in-vitro development of frozen-thawed mouse embyros.
• Expression of MUC2 epithelial mucin in breast carcinoma.
• Glucose utilization by enzymatically-formed trophoblastic vesicles and Day-14 porcine blastocysts.
• In vitro culture and maintenance of trophoblastic vesicles: Observations on the development of contractile structures with concurrent vessel development.
• Induction of estrus and superovulation in seasonally anestrous ewes.
• Non-surgical embryo transfer in cattle embryo-recipient interactions.
• Observations on the in vitro formation, development and differentiation of trilaminar vesicles formed from enzymatically dispersed porcine blastocysts.
• One-step sucrose dilution of frozen-thawed sheep embryos.
• Osteotomy for distal radius malunion.
• Pattern of sex steroids secretion and their relationship with embryo yield in Jersey cows superovulated with PMSG.
• Possible "immuno-protection" of the bovine parvovirus in the uterus: Preliminary communication.
• Quantity and quality of embryos collected from mice passively immunized against estradiol.
• Sucrose dilution of frozen mouse embryos: Interaction of glycerol and sucrose concentrations.
• Superovulation of cattle with equine pituitary extract and porcine ESH.
• Superovulation of progestagen synchronized ewes.
• Supervoulation of goats with FSH or FSH + LH following synchronization of estrus.
• The ability of fetal calf serum, new-born calf serum and normal steer serum to promote the in vitro development of bovine morulae.
• The effects of FSH-priming and dominant follicular regression on the superovulatory response of cattle.
• The use of tympanocentesis in the diagnosis and management of acute otitis media.