Induction of autophagic cell death in Leishmania donovani by antimicrobial peptides.

Induction of autophagic cell death in Leishmania donovani by antimicrobial peptides. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Induction of autophagic cell death in Leishmania donovani by antimicrobial peptides. Abstract Text:

Ananya Bera,Shashi Singh,Ramakrishnan Nagaraj,Tushar Vaidya,

We demonstrate that antimicrobial peptides induce an autophagic cell death in the protozoan pathogen, Leishmania donovani. In our study, three antimicrobial peptides, Indolicidin, and two peptides derived from Seminalplasmin exhibit antileishmanial activity with a 50% lethal dose of 3.5 x 10(-5), 3.8 x 10(-4) and 1.7 x 10(-8) microM, respectively. The action of these antimicrobial peptides on the Leishmania cell involves ionic interactions, which are modulated by lipophosphoglycan on the parasite's surface. Peptide treatment caused dissipation of membrane potential and equilibration of intracellular pH with extracellular environment. However, there was no release of intracellular GFP molecules upon peptide treatment of a GFP expressing Leishmania clone. Transmission electron microscopic studies show extensive intracellular damage including cytoplasmic vacuolization and degeneration of cellular organization without disruption of the plasma membrane. These peptides induce cell death via a non-apoptotic process as shown by lack of nuclear fragmentation or DNA laddering and independent of caspase-like activity. Instead, Monodansylcadaverine (MDC), a biochemical marker of autophagy specifically labels the vacuoles induced by peptides. Collectively, these results indicate that in addition to their effects on the leishmanial membrane, these antimicrobial peptides induce pathway(s) for autophagic cell death in L. donovani.

Induction of autophagic cell death in Leishmania donovani by antimicrobial peptides. Publishing Authors By Initials

A Bera,S Singh,R Nagaraj,T Vaidya,

For similar proteins: seminal plasma proteins: seminal vesicle secretory proteins research abstracts see: proteins: seminal plasma proteins: seminal vesicle secretory proteins research

PUBMED ID PMID:

MEDLINE DATE:

Induction of autophagic cell death in Leishmania donovani by antimicrobial peptides. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Molecular and biochemical parasitology

VOLUME: 127

Page Numbers: 23-35

Journal Abbreviation: Mol. Biochem. Parasitol.

ISSN: 0166-6851

DAY: 19

MONTH: Mar

YEAR: 2003

Induction of autophagic cell death in Leishmania donovani by antimicrobial peptides. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 8006324

Induction of autophagic cell death in Leishmania donovani by antimicrobial peptides. Keywords Mesh Terms:

KEYWORDS: Seminal Vesicle Secretory Proteins

MESH TERMS: pharmacology

Chemical & Substance for Abstract: Induction of autophagic cell death in Leishmania donovani by antimicrobial peptides. Information

Substance Name: indolicidin

Registry Number: 140896-21-5

Grant and Affiliation Information for Induction of autophagic cell death in Leishmania donovani by antimicrobial peptides.

AFFILIATION: Centre for Cellular and Molecular Biology, Uppal Road, Hyderabad 500007, India.

Country: Netherlands

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: Mol Biochem Parasitol

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Induction of autophagic cell death in Leishmania donovani by antimicrobial peptides Related Publications

• A POINT IN FAVOUR OF PROFESSOR ARTHUR THOMSON'S THEORY OF THE PRODUCTION OF GLAUCOMA.
• Acute renal failure secondary to oxalosis in a recipient of a simultaneous kidney-pancreas transplant: was mycophenolate the cause?
• Analysis of gross deletions in the MEN1 gene in patients with multiple endocrine neoplasia type 1.
• Anti-phospholipid antibody-mediated rejection.
• Antidepressant treatments regulate matrix metalloproteinases-2 and -9 (MMP-2/MMP-9) and tissue inhibitors of the metalloproteinases (TIMPS 1-4) in the adult rat hippocampus.
• Atypical neural substrates of Embedded Figures Task performance in children with Autism Spectrum Disorder.
• Biomechanical analysis of differing pedicle screw insertion angles.
• Carpopedal spasm in an elderly man: an unusual presentation of coeliac disease.
• Clinical importance of anti-human leukocyte antigen-specific antibody concentration in performing calculated panel reactive antibody and virtual crossmatches.
• Cocirculation of and coinfections with hepatitis A virus subgenotypes IIIA and IB in patients from Pune, western India.
• Development of a real-time polymerase chain reaction assay for the quantification of Leishmania species and the monitoring of systemic distribution of the pathogen.
• Developmental differences in cognitive control of socio-affective processing.
• Early antiretroviral therapy: The pendulum shifts again.
• Effects of atypical anxiolytic N-phenyl-2-[1-[3-(2-pyridinylethynyl)benzoyl]-4-piperidine]acetamide (JNJ-5234801) on alcohol intake in alcohol-preferring P rats.
• Induction of autophagic cell death in Leishmania donovani by antimicrobial peptides.
• Influence of fluoride containing toothpaste on serum and urine fluoride levels in children.
• Mathematical modelling of radiotherapy strategies for early breast cancer.
• Method to test whether late extended letrozole, rather than self- selection, improves the outcome in patients with breast cancer who have completed 5 years of tamoxifen.
• Mitochondrial drug targets in apicomplexan parasites.
• Novel technologies for the discovery and quantitation of biomarkers of toxicity.
• Plasmodium vivax malaria in spite of atovaquone/proguanil (malarone) prophylaxis.
• Platelet Inhibition by Aspirin 81 and 325 mg/day in Men Versus Women Without Clinically Apparent Cardiovascular Disease.
• Platelet functional implications of glycoprotein Ibalpha polymorphisms in African Americans.
• Polymer platforms for drug delivery and biomedical imaging.
• Psychiatry Clerkship Objective Structured Clinical Examination is Here to Stay.
• Pyridine biodegradation in a novel rotating rope bioreactor.
• Racial differences of lipoprotein subclass distributions in postmenopausal women.
• Re: Declines in invasive breast cancer and use of postmenopausal hormone therapy in a screening mammography population.
• Stressor-specific regulation of distinct brain-derived neurotrophic factor transcripts and cyclic AMP response element-binding protein expression in the postnatal and adult rat hippocampus.
• Vitamin D and cancer.