Induction of apoptosis by transiently transfected metabolically stable wt p53 in transformed cell lines.

Induction of apoptosis by transiently transfected metabolically stable wt p53 in transformed cell lines. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Induction of apoptosis by transiently transfected metabolically stable wt p53 in transformed cell lines. Abstract Text:

E Yonish-Rouach,J Borde,M Gotteland,Z Mishal,A Viron,E May,

Biochemical and functional properties of wild-type (wt) and mutant p53 were studied under the same cellular environment by transient transfection. Exogenous wt p53 expressed in transformed cell lines was found to be as metabolically stable as mutant p53. Yet only mutant p53 bound to hsp70 whereas wt p53 did not, suggesting that the metabolic stability of p53 does not depend on its ability to form complexes with hsp70. The wt protein was expressed essentially in the nucleus, while mutant p53 showed both nuclear and cytoplasmic expression, as determined by immunofluorescence staining with PAb122. In addition, staining with PAb1801 revealed a number of strongly fluorescent cell fragments in cultures transfected by wt p53. Morphological features of apoptosis were observed in these cultures. Quantitative analysis by flow cytometry confirmed that only the cell population expressing wt p53 had a significant amount of cell debris. Thus, transient expression of a metabolically stable wt, but not mutant, p53 induces cell death by apoptosis. The present study demonstrates a model system to investigate the functional domains of p53 in the induction of apoptosis.

Induction of apoptosis by transiently transfected metabolically stable wt p53 in transformed cell lines. Publishing Authors By Initials

E Yonish-Rouach,J Borde,M Gotteland,Z Mishal,A Viron,E May,

For similar abstracts research abstracts see: abstracts research

PUBMED ID PMID:

MEDLINE DATE:

Induction of apoptosis by transiently transfected metabolically stable wt p53 in transformed cell lines. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Cell death and differentiation

VOLUME: 1

Page Numbers: 39-47

Journal Abbreviation: Cell Death Differ.

ISSN: 1350-9047

DAY: 20

MONTH: Jul

YEAR: 1994

Induction of apoptosis by transiently transfected metabolically stable wt p53 in transformed cell lines. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 9437445

Induction of apoptosis by transiently transfected metabolically stable wt p53 in transformed cell lines. Keywords Mesh Terms:

KEYWORDS:

MESH TERMS:

Chemical & Substance for Abstract: Induction of apoptosis by transiently transfected metabolically stable wt p53 in transformed cell lines. Information

Substance Name:

Registry Number:

Grant and Affiliation Information for Induction of apoptosis by transiently transfected metabolically stable wt p53 in transformed cell lines.

AFFILIATION: Laboratoire d'Oncologie Moléculaire, IRSC, CNRS, Villejuif Cedex - France.

Country: England

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: Cell Death Differ

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Induction of apoptosis by transiently transfected metabolically stable wt p53 in transformed cell lines Related Publications

• American Indian/Alaska Native alcohol-related incarceration and treatment.
• Ascorbate transport by primary cultured neurons and its role in neuronal function and protection against excitotoxicity.
• Bis-acridines as lead antiparasitic agents: structure-activity analysis of a discrete compound library in vitro.
• Bone morphogenetic protein down-regulation of neuronal pituitary adenylate cyclase-activating polypeptide and reciprocal effects on vasoactive intestinal peptide expression.
• Cell proliferation and hair tip growth in the Arabidopsis root are under mechanistically different forms of redox control.
• Constitutive activation of endocytosis by mutation of myoA, the myosin I gene of Aspergillus nidulans.
• Current status of double balloon enteroscopy with focus on the Wiesbaden results.
• Direct cell-to-cell spread of a pathogenic yeast.
• Discriminating between cellular and misfolded prion protein by using affinity to 9-aminoacridine compounds.
• Electrostatic interactions across a charged lipid bilayer.
• EuroQol (EQ-5D) measure of quality of life predicts mortality, emergency department utilization, and hospital discharge rates in HIV-infected adults under care.
• Further evidence of the reliability and validity of the premature ejaculation diagnostic tool.
• General practice. Hit and miss.
• Increased cardiac sympathetic nerve activity in heart failure is not due to desensitization of the arterial baroreflex.
• Induction of apoptosis by transiently transfected metabolically stable wt p53 in transformed cell lines.
• Interleukin-1-induced NF-kappaB activation is NEMO-dependent but does not require IKKbeta.
• JAZ mediates G1 cell-cycle arrest and apoptosis by positively regulating p53 transcriptional activity.
• Letter and category fluency in children with fetal alcohol syndrome from a community in South Africa.
• Microarray analyses of pituitary adenylate cyclase activating polypeptide (PACAP)-regulated gene targets in sympathetic neurons.
• Noncompensation in peptide/receptor gene expression and distinct behavioral phenotypes in VIP- and PACAP-deficient mice.
• Novel mitogen-activated protein kinase MpkC of Aspergillus fumigatus is required for utilization of polyalcohol sugars.
• Patients' attitudes and beliefs about back pain and its management after physiotherapy for low back pain.
• Premotor circuits controlling eyelid movements in conjunction with vertical saccades in the cat: II. interstitial nucleus of Cajal.
• Sex-specific association of the androgen to oestrogen ratio with adipocytokine levels in older adults: the Rancho Bernardo Study.
• Sex-specific determinants of serum adiponectin in older adults: the role of endogenous sex hormones.
• Spanish-speaking dairy workers in New York, Pennsylvania, and Vermont: results from a survey of farm owners.
• Sustained generation of electricity by the spore-forming, Gram-positive, Desulfitobacterium hafniense strain DCB2.
• The trefoil protein TFF1 is bound to MUC5AC in human gastric mucosa.
• The yeast Pdr5p multidrug transporter: how does it recognize so many substrates?
• [Triterpenes and Steroids in Callus Cultures of Solanum dulcamara.]