Induction of apoptosis by artemisinin relieving the severity of inflammation in caerulein-induced acute pancreatitis.

Induction of apoptosis by artemisinin relieving the severity of inflammation in caerulein-induced acute pancreatitis. Research Abstract Details 

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Induction of apoptosis by artemisinin relieving the severity of inflammation in caerulein-induced acute pancreatitis. Abstract Text:

Ming Zhao,Dong-Bo Xue,Biao Zheng,Wei-Hui Zhang,Shang-Ha Pan,Bei Sun,

AIM: To observe the apoptosis and oncosis of pancreatic acinar cells and secondary inflammatory reaction in pancreatic tissue from rats with acute pancreatitis (AP), and the influences of artemisinin on them. METHODS: AP was induced by 4 intraperitoneal injections of caerulein at 1 h intervals. To induce apoptosis, solution of artemisinin (50 mg/kg) was given intraperitoneally 1, 12, 24 and 36 h after the last caerulein injection. Histological examination of impairment of pancreatic tissue and detection of serum amylase were performed to evaluate the severity of acute pancreatitis. Apoptosis and oncosis were detected with acridine orange (AO) and ethylene dibromide (EB) staining. Caspase-3 and myeloperoxidase (MPO) activity were measured by colorimetric assay. Nuclear factor-kappa B (NF-kappaB) activation was detected by flow cytometry. Macrophage inflammatory protein-1alpha (MIP-1alpha) protein was measured by Western blot. Interleukin-1beta (IL-1beta) mRNA was detected by RT-PCR. RESULTS: Addition of artemisinin increased the number of apoptotic cells (11.7% +/- 1.4% vs 6.3% +/- 0.7%, P < 0.05), while reduced the number of oncotic cells (13.0% +/- 2.4% vs 17.5% +/- 2.2%, P < 0.05). The activity of caspase-3 speeded up (1.52 +/- 0.21 vs 1.03 +/- 0.08, P < 0.05), the pancreas pathological impairment was relieved (3.0 +/- 0.5 vs 4.0 +/- 0.5, P < 0.05) and the level of serum amylase decreased (5642 +/- 721 U/dL vs 7821 +/- 653 U/dL, P < 0.05). The activation of NF-kB (29% +/- 4.1% vs 42% +/- 5.8%), MIP-1alpha protein (3.7 +/- 0.5 vs 5.8 +/- 0.7), MPO (0.52 +/- 0.06 U/g vs 0.68 +/- 0.09 U/g), IL-1beta mRNA (1.7 +/- 0.3 vs 2.4 +/- 0.4) in the apoptosis inducing group was obviously decreased (P < 0.05). CONCLUSION: Inducing apoptosis can relieve pathological impairment and inflammatory reaction in AP rats.

Induction of apoptosis by artemisinin relieving the severity of inflammation in caerulein-induced acute pancreatitis. Publishing Authors By Initials

M Zhao,DB Xue,B Zheng,WH Zhang,SH Pan,B Sun,

For similar animals: chordata: vertebrates: mammals: rodentia: muridae: murinae: rats: rats, wistar research abstracts see: animals: chordata: vertebrates: mammals: rodentia: muridae: murinae: rats: rats, wistar research

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Induction of apoptosis by artemisinin relieving the severity of inflammation in caerulein-induced acute pancreatitis. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: World journal of gastroenterology : WJG

VOLUME: 13

Page Numbers: 5612-7

Journal Abbreviation: World J. Gastroenterol.

ISSN: 1007-9327

DAY: 14

MONTH: Nov

YEAR: 2007

Induction of apoptosis by artemisinin relieving the severity of inflammation in caerulein-induced acute pancreatitis. Information

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LANGUAGE: eng

NlmUniqueID: 100883448

Induction of apoptosis by artemisinin relieving the severity of inflammation in caerulein-induced acute pancreatitis. Keywords Mesh Terms:

KEYWORDS: Rats, Wistar

MESH TERMS: analysis

Chemical & Substance for Abstract: Induction of apoptosis by artemisinin relieving the severity of inflammation in caerulein-induced acute pancreatitis. Information

Substance Name: Caspase 3

Registry Number: EC 3.4.22.-

Grant and Affiliation Information for Induction of apoptosis by artemisinin relieving the severity of inflammation in caerulein-induced acute pancreatitis.

AFFILIATION: Department of General Surgery, First Clinical College, Harbin Medical University, Harbin 150001, Heilongjiang Province, China.

Country: China

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MEDLINETA: World J Gastroenterol

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