Induction of acetylcholinesterase release from erythrocytes in the presence of liposomes.

Induction of acetylcholinesterase release from erythrocytes in the presence of liposomes. Research Abstract Details 

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Induction of acetylcholinesterase release from erythrocytes in the presence of liposomes. Abstract Text:

K Suzuki,Y Okumura,J Sunamoto,

When human erythrocytes are incubated with liposomes, the release of acetylcholinesterase (AChE) occurs following an induction period [Cook et al. (1980) Biochemistry 19, 4601-4607]. However, the mechanism of the induction has not been elucidated. We examined the relationships among the lipid transfer from liposomes to erythrocytes, the morphological change of erythrocytes, the fluidity of the erythrocyte membrane and the start of AChE release. The AChE release into the liposomes and into shed-vesicle fractions started simultaneously after an induction period. The morphological index (MI) of erythrocytes was approximately 2.8 at the beginning of the release, regardless of the induction period. AChE was not released from the erythrocytes of index 2.8 even in the presence of liposomes if the MI remained at 2.8. Therefore, for the release, erythrocytes needed a further increase of the MI from 2.8. As the rate of lipid transfer increased, the induction period became shorter. No significant lipid release from erythrocytes was detected during the induction period. The initiation of the AChE release was not simply affected by the change in the membrane fluidity of erythrocytes upon interaction with liposomes. These results first demonstrate that AChE release into the shed-vesicle and liposome fractions is triggered by a further increase of the MI from 2.8, which is induced by lipid transfer from liposomes to erythrocytes.

Induction of acetylcholinesterase release from erythrocytes in the presence of liposomes. Publishing Authors By Initials

K Suzuki,Y Okumura,J Sunamoto,

For similar biochemical phenomena, metabolism, and nutrition: biochemical phenomena: membrane fluidity research abstracts see: biochemical phenomena, metabolism, and nutrition: biochemical phenomena: membrane fluidity research

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Induction of acetylcholinesterase release from erythrocytes in the presence of liposomes. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Journal of biochemistry

VOLUME: 125

Page Numbers: 876-82

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: May

YEAR: 1999

Induction of acetylcholinesterase release from erythrocytes in the presence of liposomes. Information

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LANGUAGE: eng

NlmUniqueID: 376600

Induction of acetylcholinesterase release from erythrocytes in the presence of liposomes. Keywords Mesh Terms:

KEYWORDS: Membrane Fluidity

MESH TERMS: enzymology

Chemical & Substance for Abstract: Induction of acetylcholinesterase release from erythrocytes in the presence of liposomes. Information

Substance Name: Acetylcholinesterase

Registry Number: EC 3.1.1.7

Grant and Affiliation Information for Induction of acetylcholinesterase release from erythrocytes in the presence of liposomes.

AFFILIATION: Department of Synthetic Chemistry and Biological Chemistry, Graduate School of Engineering, Kyoto University, Kyoto, Japan. ksuzuki@bio.nagoya-u.ac.jp

Country: JAPAN

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MEDLINETA: J Biochem

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