Induction and high density culture of human hepatoblasts from fetal hepatocytes with suppressing transformation.

Induction and high density culture of human hepatoblasts from fetal hepatocytes with suppressing transformation. Research Abstract Details 

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Induction and high density culture of human hepatoblasts from fetal hepatocytes with suppressing transformation. Abstract Text:

Akifumi Kiyota,Taku Matsushita,Ryuichi Ueoka,Akifumi Kiyota,Taku Matsushita,Ryuichi Ueoka,Akifumi Kiyota,Taku Matsushita,Ryuichi Ueoka,

It is well known that it would be important to cultivate human hepatocytes of about 10(10) cells at a high cell density, about 1 x 10(7) cells/cm(3), in the bioreactor for the development of bioartificial liver. However, since primary human hepatocytes lack an ability to proliferate in vitro, it is essential to establish a culture method for the proliferation of normal human hepatic stem cells as a cell source. In this study, it was found that human hepatoblasts, a kind of hepatic stem cells, were induced from human fetal hepatocytes while keeping the ability of proliferation by the treatment of 1mM sodium butyrate (SB) for 12 d of culture. The transformation of hepatoblasts was evaluated by abnormal prothrombin (PIVKA-II) assay, which is a clinical marker for hepatocellular carcinoma. The PIVKA-II production rate of the cells was suppressed to the normal level under 1 mM SB. The cells including hepatoblasts under 1 mM SB attached to the porous hydroxyapatite carriers and proliferated to a high cell density of about 1 x 10(7) cells/cm(3) in the carriers. The liver-specific function, cytochrome P450 3A4 activity (4.2 pmol/mg protein/min) of the cells in the carriers under 1 mM SB was comparable to that of primary human hepatocytes. Ammonia metabolizing activity (0.21 micromol/10(6) cells/h) of the cells was also comparable to that of porcine hepatocytes used in the bioartificial liver. The PIVKA-II production rate of the cells in the carrier was suppressed to the normal level. These results suggested that induction of human hepatoblasts from fetal hepatocytes by the treatment of 1mM SB and proliferation of the cells at a high cell density using hydroxyapatite carriers should be one of the more promising culture methods for bioartificial liver developments.

Induction and high density culture of human hepatoblasts from fetal hepatocytes with suppressing transformation. Publishing Authors By Initials

A Kiyota,T Matsushita,R Ueoka,A Kiyota,T Matsushita,R Ueoka,A Kiyota,T Matsushita,R Ueoka,

For similar diagnosis: diagnostic techniques and procedures: diagnostic imaging: microscopy: microscopy, electron: microscopy, electron, scanning research abstracts see: diagnosis: diagnostic techniques and procedures: diagnostic imaging: microscopy: microscopy, electron: microscopy, electron, scanning research

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Induction and high density culture of human hepatoblasts from fetal hepatocytes with suppressing transformation. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Biological & pharmaceutical bulletin

VOLUME: 30

Page Numbers: 2308-11

Journal Abbreviation: Biol. Pharm. Bull.

ISSN: 0918-6158

DAY: 2

MONTH: Dec

YEAR: 2007

Induction and high density culture of human hepatoblasts from fetal hepatocytes with suppressing transformation. Information

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LANGUAGE: eng

NlmUniqueID: 9311984

Induction and high density culture of human hepatoblasts from fetal hepatocytes with suppressing transformation. Keywords Mesh Terms:

KEYWORDS: Microscopy, Electron, Scanning

MESH TERMS: physiology

Chemical & Substance for Abstract: Induction and high density culture of human hepatoblasts from fetal hepatocytes with suppressing transformation. Information

Substance Name: CYP3A43 protein, human

Registry Number: EC 1.14.14.1

Grant and Affiliation Information for Induction and high density culture of human hepatoblasts from fetal hepatocytes with suppressing transformation.

AFFILIATION: Division of Applied Life Science, Graduate School of Engineering, Sojo University, 4-22-1 Ikeda, Kumamoto 860-0082, Japan.

Country: Japan

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MEDLINETA: Biol Pharm Bull

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