Indole-3-carbinol mediated cell cycle arrest of LNCaP human prostate cancer cells requires the induced production of activated p53 tumor suppressor protein.

Indole-3-carbinol mediated cell cycle arrest of LNCaP human prostate cancer cells requires the induced production of activated p53 tumor suppressor protein. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Indole-3-carbinol mediated cell cycle arrest of LNCaP human prostate cancer cells requires the induced production of activated p53 tumor suppressor protein. Abstract Text:

Jocelyn C Hsu,Anurupa Dev,Aimee Wing,Christine T Brew,Leonard F Bjeldanes,Gary L Firestone,

Indole-3-carbinol (I3C), a dietary compound found naturally in cruciferous vegetables of the Brassica genus such as broccoli and brussels sprouts, induces a G1 growth arrest of human reproductive cancer cells. We previously reported that in LNCaP prostate cancer cells, I3C down-regulated cyclin-dependent kinase (CDK) 2 activity. In our current study, Western blotting and quantitative RT-PCR demonstrated that I3C treatment increased both the transcripts and protein levels of the CDK2 inhibitor p21(waf1/cip1) (p21). Transfection of luciferase reporter plasmids containing wild-type and mutated p21 promoter fragments revealed that I3C induced p21 gene transcription through a p53 DNA binding element. Oligonucleotide precipitation showed that I3C increased the level of activated p53 nuclear protein that is competent to bind its DNA target site on the p21 promoter. Ablation of p53 production using short interfering RNA (siRNA) prevented that the I3C induced G1 arrest and up-regulation of p21 expression. Western blots using p53 phospho-specific antibodies revealed that I3C treatment increased the levels of three phosphorylated forms of p53 (Ser15, Ser37, Ser392) that are known to contribute to p53 protein stability and greater transactivation potential. Taken together, our results establish that the I3C induced G1 arrest of human prostate cancer cells requires the induced production of the activated phosphorylated forms of p53, which stimulate transcription of the CDK2 inhibitor p21.

Indole-3-carbinol mediated cell cycle arrest of LNCaP human prostate cancer cells requires the induced production of activated p53 tumor suppressor protein. Publishing Authors By Initials

JC Hsu,A Dev,A Wing,CT Brew,LF Bjeldanes,GL Firestone,

For similar genetic processes: gene expression regulation: up-regulation research abstracts see: genetic processes: gene expression regulation: up-regulation research

PUBMED ID PMID:

MEDLINE DATE:

Indole-3-carbinol mediated cell cycle arrest of LNCaP human prostate cancer cells requires the induced production of activated p53 tumor suppressor protein. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: Biochemical pharmacology

VOLUME: 72

Page Numbers: 1714-23

Journal Abbreviation: Biochem. Pharmacol.

ISSN: 0006-2952

DAY: 12

MONTH: 09

YEAR: 2006

Indole-3-carbinol mediated cell cycle arrest of LNCaP human prostate cancer cells requires the induced production of activated p53 tumor suppressor protein. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 101032

Indole-3-carbinol mediated cell cycle arrest of LNCaP human prostate cancer cells requires the induced production of activated p53 tumor suppressor protein. Keywords Mesh Terms:

KEYWORDS: Up-Regulation

MESH TERMS: metabolism

Chemical & Substance for Abstract: Indole-3-carbinol mediated cell cycle arrest of LNCaP human prostate cancer cells requires the induced production of activated p53 tumor suppressor protein. Information

Substance Name: Luciferases

Registry Number: EC 1.13.12.-

Grant and Affiliation Information for Indole-3-carbinol mediated cell cycle arrest of LNCaP human prostate cancer cells requires the induced production of activated p53 tumor suppressor protein.

AFFILIATION: Department of Molecular and Cell Biology and The Cancer Research Laboratory, The University of California at Berkeley, Berkeley, CA 94720, USA.

Country: England

AGENCY: United States NCI

GRANT: CA102360

ACRONYM: CA

MEDLINETA: Biochem Pharmacol

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Indole-3-carbinol mediated cell cycle arrest of LNCaP human prostate cancer cells requires the induced production of activated p53 tumor suppressor protein Related Publications

• "No party hat, no party": successful condom use in sex work in Mexico and the dominican republic.
• Assuring the integrity of olive oil products.
• Convergent strabismus corrected by lenses and orthoptics; a case report.
• DEVD-NucView488: a novel class of enzyme substrates for real-time detection of caspase-3 activity in live cells.
• Detection of trace fatty acids in fats and oils by urea fractionation and gas-liquid chromatography.
• Direct graphite furnace-atomic absorption method for determination of lead in edible oils and fats: summary of collaborative study.
• ETIOLOGICAL FACTORS IN CHRONIC COUGH: AN ANALYSIS OF ONE HUNDRED CASES-PRELIMINARY REPORT.
• Electron density mapping of triblock copolymers associated with model biomembranes: insights into conformational states and effect on bilayer structure.
• Expression of the serum- and glucocorticoid-inducible protein kinase, Sgk, is a cell survival response to multiple types of environmental stress stimuli in mammary epithelial cells.
• Gel-permeation liquid chromatographic method for determination of polymerized triglycerides in oils and fats: summary of collaborative study.
• Glucocorticoid-induced degradation of glycogen synthase kinase-3 protein is triggered by serum- and glucocorticoid-induced protein kinase and Akt signaling and controls beta-catenin dynamics and tight junction formation in mammary epithelial tumor cells.
• INFORMATION: HEALTH INSURANCE AND MEDICAL PRACTICE IN GERMANY.
• Improving spatial representation of global ship emissions inventories.
• Indole-3-carbinol mediated cell cycle arrest of LNCaP human prostate cancer cells requires the induced production of activated p53 tumor suppressor protein.
• Indole-3-carbinol selectively uncouples expression and activity of estrogen receptor subtypes in human breast cancer cells.
• Liquid chromatographic method for determination of triglycerides in vegetable oils in terms of their partition numbers: summary of collaborative study.
• N-Alkoxy derivatization of indole-3-carbinol increases the efficacy of the G1 cell cycle arrest and of I3C-specific regulation of cell cycle gene transcription and activity in human breast cancer cells.
• Needle lung biopsy, bronchial brushing and mediastinoscopy in management of chest diseases.
• Neurotoxicity of pesticides.
• Prevention and relief of stenosis of lacrimal duct.
• Studies with vitamin E and beta-carotene as single agents do not prove lack of benefit.
• The stimulus-dependent co-localization of serum- and glucocorticoid-regulated protein kinase (Sgk) and Erk/MAPK in mammary tumor cells involves the mutual interaction with the importin-alpha nuclear import protein.
• Thermoresponsive nanostructures by self-assembly of a poly(N-isopropylacrylamide)-lipid conjugate.
• Toluene diffusion and reaction in unsaturated Pseudomonas putida biofilms.
• VACCINE THERAPY IN INFECTIOUS BRONCHITIS AND ASTHMA.