Increasing protein conformational stability by optimizing beta-turn sequence.

Increasing protein conformational stability by optimizing beta-turn sequence. Research Abstract Details 

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Increasing protein conformational stability by optimizing beta-turn sequence. Abstract Text:

Saul R Trevino,Stephanie Schaefer,J Martin Scholtz,C Nick Pace,Saul R Trevino,Stephanie Schaefer,J Martin Scholtz,C Nick Pace,

Protein conformational stability is an important concern in many fields. Here, we describe a strategy for significantly increasing conformational stability by optimizing beta-turn sequence. Proline and glycine residues are statistically preferred at several beta-turn positions, presumably because their unique side-chains contribute favorably to conformational stability in certain beta-turn positions. However, beta-turn sequences often deviate from preferred proline or preferred glycine. Therefore, our strategy involves replacing non-proline and non-glycine beta-turn residues with preferred proline or preferred glycine residues. Here, we develop guidelines for selecting appropriate mutations, and present results for five mutations (S31P, S42G, S48P, T76P, and Q77G) that significantly increase the conformational stability of RNase Sa. The increases in stability ranged from 0.7 kcal/mol to 1.3 kcal/mol. The strategy was successful in overlapping or isolated beta-turns, at buried (up to 50%) or completely exposed sites, and at relatively flexible or inflexible sites. Considering the significant number of beta-turn residues in every globular protein and the frequent deviation of beta-turn sequences from preferred proline and preferred glycine residues, this simple, efficient strategy will be useful for increasing the conformational stability of proteins.

Increasing protein conformational stability by optimizing beta-turn sequence. Publishing Authors By Initials

SR Trevino,S Schaefer,JM Scholtz,CN Pace,SR Trevino,S Schaefer,JM Scholtz,CN Pace,

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Increasing protein conformational stability by optimizing beta-turn sequence. Journal Published:

PUBLICATION TYPE: Research Support, U.S. Gov't,

Journal: Journal of molecular biology

VOLUME: 373

Page Numbers: 211-8

Journal Abbreviation: J. Mol. Biol.

ISSN: 0022-2836

DAY: 9

MONTH: 08

YEAR: 2007

Increasing protein conformational stability by optimizing beta-turn sequence. Information

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LANGUAGE: eng

NlmUniqueID: 2985088

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Grant and Affiliation Information for Increasing protein conformational stability by optimizing beta-turn sequence.

AFFILIATION: Department of Molecular and Cellular Medicine, Texas A&M University System Health Science Center, College Station, TX 77843, USA.

Country: England

AGENCY: United States NIGMS

GRANT: T32 GM 065088

ACRONYM: GM

MEDLINETA: J Mol Biol

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