Increased motion and travel, rather than stable docking, characterize the last moments before secretory granule fusion.

Increased motion and travel, rather than stable docking, characterize the last moments before secretory granule fusion. Research Abstract Details 

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Increased motion and travel, rather than stable docking, characterize the last moments before secretory granule fusion. Abstract Text:

Vadim E Degtyar,Miriam W Allersma,Daniel Axelrod,Ronald W Holz,

The state of secretory granules immediately before fusion with the plasma membrane is unknown, although the granules are generally assumed to be stably bound (docked). We had previously developed methods using total internal reflection fluorescence microscopy and image analysis to determine the position of chromaffin granules immediately adjacent to the plasma membrane with high precision, often to within approximately 10 nm, or <5% of the granule diameter (300 nm). These distances are of the dimensions of large proteins and are comparable with the unitary step sizes of molecular motors. Here we demonstrate with quantitative measures of granule travel in the plane parallel to the plasma membrane that secretory granules change position within several hundred milliseconds of nicotinic agonist-induced fusion. Furthermore, just before fusion, granules frequently move to areas that they have rarely visited. The movement of granules to new areas is most evident for granules that fuse later during the stimulus. The movement may increase the probability of productive interactions of the granule with the plasma membrane or may reflect the pull of molecular interactions between the granule and the plasma membrane that are part of the fusion process. Thus, instead of being stably docked before exocytosis, granules undergo molecular-scale motions and travel immediately preceding the fusion event.

Increased motion and travel, rather than stable docking, characterize the last moments before secretory granule fusion. Publishing Authors By Initials

VE Degtyar,MW Allersma,D Axelrod,RW Holz,

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Increased motion and travel, rather than stable docking, characterize the last moments before secretory granule fusion. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: Proceedings of the National Academy of Sciences of

VOLUME: 104

Page Numbers: 15929-34

Journal Abbreviation: Proc. Natl. Acad. Sci. U.S.A.

ISSN: 0027-8424

DAY: 24

MONTH: 09

YEAR: 2007

Increased motion and travel, rather than stable docking, characterize the last moments before secretory granule fusion. Information

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LANGUAGE: eng

NlmUniqueID: 7505876

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Grant and Affiliation Information for Increased motion and travel, rather than stable docking, characterize the last moments before secretory granule fusion.

AFFILIATION: Department of Pharmacology, University of Michigan, Ann Arbor, MI 48104-0632, USA.

Country: United States

AGENCY: United States NINDS

GRANT: R01 NS38129

ACRONYM: NS

MEDLINETA: Proc Natl Acad Sci U S A

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