Increased Melanogenesis is a Risk Factor for Oxidative DNA Damage- Study on Cultured Melanocytes and Atypical Nevus Cells.

Increased Melanogenesis is a Risk Factor for Oxidative DNA Damage- Study on Cultured Melanocytes and Atypical Nevus Cells. Research Abstract Details 

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Increased Melanogenesis is a Risk Factor for Oxidative DNA Damage- Study on Cultured Melanocytes and Atypical Nevus Cells. Abstract Text:

Melanin synthesis is an oxygen-dependent process that acts as a potential source of reactive oxygen species (ROS) inside pigment-forming cells. The synthesis of the lighter variant of melanin, pheomelanin, consumes cysteine and this may limit the capacity of the cellular antioxidative defense. We show that tyrosine-induced melanogenesis in cultured normal human melanocytes (NHM) is accompanied by increased production of ROS and decreased concentration of intracellular glutathione. Clinical atypical (dysplastic) nevi (DN) regularly contain more melanin than do normal melanocytes (MC). We also show that in these cultured DN cells three out of four exhibit elevated synthesis of pheomelanin and this is accompanied by their early senescence. By using various redox-sensitive molecular probes, we demonstrate that cultured DN cells produce significantly more ROS than do normal MC from the same donor. Our experiments employing single-cell gel electrophoresis (comet assay) usually reveal higher fragmentation of DNA in DN cells than in normal MC. Even if in some cases the normal alkaline comet assay shows no differences in DNA fragmentation between DN cells and normal MC, the use of the comet assay with formamidopyrimidine DNA glycosylase can disclose that the DNA of the cultured DN cells harbor more oxidative damage than the DNA of normal MC from the same person.

Increased Melanogenesis is a Risk Factor for Oxidative DNA Damage- Study on Cultured Melanocytes and Atypical Nevus Cells. Publishing Authors By Initials

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MEDLINE DATE: 2008 May-Jun

Increased Melanogenesis is a Risk Factor for Oxidative DNA Damage- Study on Cultured Melanocytes and Atypical Nevus Cells. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Photochemistry and photobiology

VOLUME: 84

Page Numbers: 550-5

Journal Abbreviation: Photochem. Photobiol.

ISSN: 0031-8655

DAY: 25

MONTH: 04

YEAR: 2008

Increased Melanogenesis is a Risk Factor for Oxidative DNA Damage- Study on Cultured Melanocytes and Atypical Nevus Cells. Information

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LANGUAGE: eng

NlmUniqueID: 376425

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Grant and Affiliation Information for Increased Melanogenesis is a Risk Factor for Oxidative DNA Damage- Study on Cultured Melanocytes and Atypical Nevus Cells.

AFFILIATION: Department of Clinical Chemistry, Leiden University Medical Center, Leiden, The Netherlands.

Country: United States

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MEDLINETA: Photochem Photobiol

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