Incorporation of nonmethyl branches by isoprenoid-like logic: multiple beta-alkylation events in the biosynthesis of myxovirescin A1.

Incorporation of nonmethyl branches by isoprenoid-like logic: multiple beta-alkylation events in the biosynthesis of myxovirescin A1. Research Abstract Details 

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Incorporation of nonmethyl branches by isoprenoid-like logic: multiple beta-alkylation events in the biosynthesis of myxovirescin A1. Abstract Text:

Christopher T Calderone,David F Iwig,Pieter C Dorrestein,Neil L Kelleher,Christopher T Walsh,Christopher T Calderone,David F Iwig,Pieter C Dorrestein,Neil L Kelleher,Christopher T Walsh,

Several polyketide secondary metabolites are predicted to undergo isoprenoid-like beta-alkylations during biosynthesis. One such secondary metabolite is myxovirescin A1, produced by Myxococcus xanthus. Myxovirescin is of special interest in that it appears to undergo two distinct beta-alkylations. Additionally, the myxovirescin biosynthetic gene cluster lacks tandem thiolation domains required in the synthesis of other beta-branched secondary metabolites. To probe the origins of the beta-branches in myxovirescin, we heterologously overexpressed the proteins predicted to be responsible for myxovirescin beta-alkylation and reconstituted their activities in vitro on model substrates. Our results confirm that myxovirescin undergoes two isoprenoid-like beta-alkylations during its biosynthesis, including an unprecedented beta-ethylation. The study of its biosynthesis should shed light on the scope and requirements for isoprenoid-like biosynthetic logic in a polyketide context.

Incorporation of nonmethyl branches by isoprenoid-like logic: multiple beta-alkylation events in the biosynthesis of myxovirescin A1. Publishing Authors By Initials

CT Calderone,DF Iwig,PC Dorrestein,NL Kelleher,CT Walsh,CT Calderone,DF Iwig,PC Dorrestein,NL Kelleher,CT Walsh,

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Incorporation of nonmethyl branches by isoprenoid-like logic: multiple beta-alkylation events in the biosynthesis of myxovirescin A1. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Chemistry & biology

VOLUME: 14

Page Numbers: 835-46

Journal Abbreviation: Chem. Biol.

ISSN: 1074-5521

DAY: 27

MONTH: Jul

YEAR: 2007

Incorporation of nonmethyl branches by isoprenoid-like logic: multiple beta-alkylation events in the biosynthesis of myxovirescin A1. Information

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LANGUAGE: eng

NlmUniqueID: 9500160

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Grant and Affiliation Information for Incorporation of nonmethyl branches by isoprenoid-like logic: multiple beta-alkylation events in the biosynthesis of myxovirescin A1.

AFFILIATION: Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, MA 02115, USA.

Country: England

AGENCY: United States NIGMS

GRANT: GM081743

ACRONYM: GM

MEDLINETA: Chem Biol

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