Incorporation of deoxyribonucleotides and ribonucleotides by a dNTP-binding cleft mutated reverse transcriptase in hepatitis B virus core particles.

Incorporation of deoxyribonucleotides and ribonucleotides by a dNTP-binding cleft mutated reverse transcriptase in hepatitis B virus core particles. Research Abstract Details 

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Incorporation of deoxyribonucleotides and ribonucleotides by a dNTP-binding cleft mutated reverse transcriptase in hepatitis B virus core particles. Abstract Text:

Hee-Young Kim,Hye-Young Kim,Jaesung Jung,Sun Park,Ho-Joon Shin,Kyongmin Kim,Hee-Young Kim,Hye-Young Kim,Jaesung Jung,Sun Park,Ho-Joon Shin,Kyongmin Kim,Hee-Young Kim,Hye-Young Kim,Jaesung Jung,Sun Park,Ho-Joon Shin,Kyongmin Kim,

Our recent observation that hepatitis B virus (HBV) DNA polymerase (P) might initiate minus-strand DNA synthesis without primer [Kim et al., (2004) Virology 322, 22-30], raised a possibility that HBV P protein may have the potential to function as an RNA polymerase. Thus, we mutated Phe 436, a bulky amino acid with aromatic side chain, at the putative dNTP-binding cleft in reverse transcriptase (RT) domain of P protein to smaller amino acids (Gly or Val), and examined RNA polymerase activity. HBV core particles containing RT dNTP-binding cleft mutant P protein were able to incorporate (32)P-ribonucleotides, but not HBV core particles containing wild type (wt), priming-deficient mutant, or RT-deficient mutant P proteins. Since all the experiments were conducted with core particles isolated from transfected cells, our results indicate that the HBV RT mutant core particles containing RT dNTP-binding cleft mutant P protein could incorporate both deoxyribonucleotides and ribonucleotides in replicating systems.

Incorporation of deoxyribonucleotides and ribonucleotides by a dNTP-binding cleft mutated reverse transcriptase in hepatitis B virus core particles. Publishing Authors By Initials

HY Kim,HY Kim,J Jung,S Park,HJ Shin,K Kim,HY Kim,HY Kim,J Jung,S Park,HJ Shin,K Kim,HY Kim,HY Kim,J Jung,S Park,HJ Shin,K Kim,

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Incorporation of deoxyribonucleotides and ribonucleotides by a dNTP-binding cleft mutated reverse transcriptase in hepatitis B virus core particles. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Virology

VOLUME: 370

Page Numbers: 205-12

Journal Abbreviation: Virology

ISSN: 0042-6822

DAY: 27

MONTH: 09

YEAR: 2007

Incorporation of deoxyribonucleotides and ribonucleotides by a dNTP-binding cleft mutated reverse transcriptase in hepatitis B virus core particles. Information

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LANGUAGE: eng

NlmUniqueID: 110674

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Grant and Affiliation Information for Incorporation of deoxyribonucleotides and ribonucleotides by a dNTP-binding cleft mutated reverse transcriptase in hepatitis B virus core particles.

AFFILIATION: Department of Microbiology, Ajou University School of Medicine, Woncheon-dong 5, Suwon 443-721, South Korea.

Country: United States

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MEDLINETA: Virology

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