Incorporation of basic fibroblast growth factor into preconfluent cultured skin substitute to accelerate neovascularisation and skin reconstruction after transplantation.

Incorporation of basic fibroblast growth factor into preconfluent cultured skin substitute to accelerate neovascularisation and skin reconstruction after transplantation. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Incorporation of basic fibroblast growth factor into preconfluent cultured skin substitute to accelerate neovascularisation and skin reconstruction after transplantation. Abstract Text:

Yasumi Tsuji-Saso,Takeshi Kawazoe,Naoki Morimoto,Yasuhiko Tabata,Tsuguyoshi Taira,Kenji Tomihata,Atsushi Utani,Shigehiko Suzuki,

Cultured skin substitutes (CSS) with both epidermal and dermal components seem to be ideal, but they have not been widely used clinically, partly because it takes several weeks to produce them. Decreasing the number of seeding cells may reduce the period required for production, but it still takes a long time before the cells become confluent and neovascularisation is completed in CSS after grafting. As we have already succeeded in reducing the number of seeded keratinocytes in this study, we first attempted to reduce the number of seeded fibroblasts. Consequently, preconfluent CSS with 10 x 10(3) cells/cm2 of fibroblasts combined with 100 x 10(3) cells/cm2 of keratinocytes could be successfully grafted on to full-thickness wounds. bFGF-impregnated gelatin microspheres were then added to the preconfluent CSS before grafting. Incorporation of bFGF significantly accelerated neovascularisation and increased epidermal thickness, cellular components, and thickness of the dermis. The incorporation of bFGF makes CSS a potential therapeutic approach for management of skin wounds.

Incorporation of basic fibroblast growth factor into preconfluent cultured skin substitute to accelerate neovascularisation and skin reconstruction after transplantation. Publishing Authors By Initials

Y Tsuji-Saso,T Kawazoe,N Morimoto,Y Tabata,T Taira,K Tomihata,A Utani,S Suzuki,

For similar abstracts research abstracts see: abstracts research

PUBMED ID PMID:

MEDLINE DATE:

Incorporation of basic fibroblast growth factor into preconfluent cultured skin substitute to accelerate neovascularisation and skin reconstruction after transplantation. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Scandinavian journal of plastic and reconstructive

VOLUME: 41

Page Numbers: 228-35

Journal Abbreviation:

ISSN: 0284-4311

DAY: 15

MONTH: 02

YEAR: 2007

Incorporation of basic fibroblast growth factor into preconfluent cultured skin substitute to accelerate neovascularisation and skin reconstruction after transplantation. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 8707869

Incorporation of basic fibroblast growth factor into preconfluent cultured skin substitute to accelerate neovascularisation and skin reconstruction after transplantation. Keywords Mesh Terms:

KEYWORDS:

MESH TERMS:

Chemical & Substance for Abstract: Incorporation of basic fibroblast growth factor into preconfluent cultured skin substitute to accelerate neovascularisation and skin reconstruction after transplantation. Information

Substance Name:

Registry Number:

Grant and Affiliation Information for Incorporation of basic fibroblast growth factor into preconfluent cultured skin substitute to accelerate neovascularisation and skin reconstruction after transplantation.

AFFILIATION: Department of Plastic and Reconstructive Surgery, Graduate School of Medicine, Kyoto University, 54 Kawahara-cho, Shogoin, Sakyo-ku, Kyoto 606-8507, Japan. saso@kuhp.kyoto-u.ac.jp

Country: Sweden

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: Scand J Plast Reconstr Surg Ha

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Incorporation of basic fibroblast growth factor into preconfluent cultured skin substitute to accelerate neovascularisation and skin reconstruction after transplantation Related Publications

• A uniformly cleaved epitaxically grown diamond crystal for synchrotron radiation.
• Absolute soft X-ray measurements using an ion chamber.
• Agmatine suppresses mesangial cell proliferation by modulating polyamine metabolism.
• Attention induces synchronization-based response gain in steady-state visual evoked potentials.
• Behavior of Medaka fish under distributed gravity.
• Branched-chain amino acid supplementation during the endoscopic injection sclerotherapy for esophageal varices may be useful for maintenance of nutritional status in patients with liver cirrhosis.
• Comprehensive genetic selection revealed essential bases in the peptidyl-transferase center.
• Cytokine production of CD8+ immune T cells but not of CD4+ T cells from Toxoplasma gondii-infected mice is polarized to a type 1 response following stimulation with tachyzoite-infected macrophages.
• Developmental capacity of bovine oocytes cryopreserved after maturation in vitro and of frozen-thawed bovine embryos derived from frozen mature oocytes.
• Developmental capacity of bovine oocytes frozen in different cryoprotectants.
• Effects of different lots of semen from the same bull on in vitro development of bovine oocytes fertilized in vitro.
• Effects of pressure on the phase transition of bilayers in liposomes. Influence of cholesterol and alpha-tocopherol.
• Elevated extracellular calcium stimulates secretion of bone morphogenetic protein 2 by a macrophage cell line.
• Expression of bHLH Transcription Factors and IGFs in the Non-sensory Patches, Olfactory Epithelium and Vomeronasal Organ.
• Fasting plasma ghrelin levels reflect malnutrition state in patients with liver cirrhosis.
• Hepatocyte-specific Pten-deficient mice as a novel model for nonalcoholic steatohepatitis and hepatocellular carcinoma.
• Hyperplasia and carcinomas in Pten-deficient mice and reduced PTEN protein in human bladder cancer patients.
• Improvement in cognitive function after radical excision of an anterior skull base meningioma - a report of 2 cases.
• Independent representation of parts and the relations between them: evidence from integrative agnosia.
• Prognosis-predicting equations using histological scores contribute to the choice of steroid therapy in IgA nephropathy: Multivariate analysis on 275 adult patients.
• Proteome analysis of glial cells treated by radiation or hydroperoxide.
• Relationship between progesterone and estradiol contents of follicular fluid and the morphological appearance of granulosa cells of follicles coexisting with corpora lutea in bovine ovaries.
• Safety of ablation of the sigmoid and transverse sinuses: an experimental study.
• Species specific differences in the ratio of short to long loop nephrons in the kidneys of laboratory rodents.
• Superovulation in the cow with a single intramuscular injection of FSH dissolved in polyvinylpyrrolidone.
• Synergism between a mycoreovirus and a hypovirus mediated by the papain-like protease p29 of the prototypic hypovirus CHV1-EP713.
• The A-site finger in 23 S rRNA acts as a functional attenuator for translocation.
• Viability of frozen-thawed bovine IVM/IVF embryos in relation to aging using various cryoprotectants.
• [High performance liquid chromatographic determination of reducing sugars in fruit juices with laser resonance Raman detection]
• [Selective extraction of organotin from biological sample by supercritical fluid extraction]