Incomplete assembly of IgA2m(2) in Chinese hamster ovary cells.

Incomplete assembly of IgA2m(2) in Chinese hamster ovary cells. Research Abstract Details 

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Incomplete assembly of IgA2m(2) in Chinese hamster ovary cells. Abstract Text:

Koteswara R Chintalacharuvu,Brian Gurbaxani,Sherie L Morrison,

Myeloma and Chinese hamster ovary (CHO) cells are frequently used for the production of recombinant antibodies. With increasing interest in producing recombinant IgA for protection against infectious agents, it is essential to characterize the IgA produced in these cells. Here we show that while myeloma cells secrete IgA2m(2) predominantly as H(2)L(2), CHO cells secrete H(2)L and H(2) in addition to fully assembled H(2)L(2). When the CHO cells also synthesize J chain and secretory component (SC), polymeric IgA and secretory IgA in which SC is disulfide bonded to the polymeric IgA are produced. Blocking cysteines on purified IgA2m(2) protein by alkylating with iodoacetamide stabilizes the disulfide bonds between the H and L chains suggesting that the disulfide bonds between H and L chains are unstable. Taken together our results suggest that the covalent assembly of IgA2m(2) is different in myeloma and CHO cells.

Incomplete assembly of IgA2m(2) in Chinese hamster ovary cells. Publishing Authors By Initials

KR Chintalacharuvu,B Gurbaxani,SL Morrison,

For similar investigative techniques: genetic techniques: gene transfer techniques: transfection research abstracts see: investigative techniques: genetic techniques: gene transfer techniques: transfection research

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Incomplete assembly of IgA2m(2) in Chinese hamster ovary cells. Journal Published:

PUBLICATION TYPE: Research Support, U.S. Gov't,

Journal: Molecular immunology

VOLUME: 44

Page Numbers: 3445-52

Journal Abbreviation: Mol. Immunol.

ISSN: 0161-5890

DAY: 27

MONTH: 04

YEAR: 2007

Incomplete assembly of IgA2m(2) in Chinese hamster ovary cells. Information

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LANGUAGE: eng

NlmUniqueID: 7905289

Incomplete assembly of IgA2m(2) in Chinese hamster ovary cells. Keywords Mesh Terms:

KEYWORDS: Transfection

MESH TERMS: metabolism

Chemical & Substance for Abstract: Incomplete assembly of IgA2m(2) in Chinese hamster ovary cells. Information

Substance Name: Recombinant Proteins

Registry Number: 0

Grant and Affiliation Information for Incomplete assembly of IgA2m(2) in Chinese hamster ovary cells.

AFFILIATION: Department of Microbiology, Immunology and Molecular Genetics and the Molecular Biology Institute, University of California Los Angeles, 405 Hilgard Avenue, Los Angeles, CA 90095, USA. kotec@lifesci.ucla.edu

Country: England

AGENCY: United States NIAID

GRANT: AI51415

ACRONYM: AI

MEDLINETA: Mol Immunol

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