Incision of trivalent chromium [Cr(III)]-induced DNA damage by Bacillus caldotenax UvrABC endonuclease.

Incision of trivalent chromium [Cr(III)]-induced DNA damage by Bacillus caldotenax UvrABC endonuclease. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Incision of trivalent chromium [Cr(III)]-induced DNA damage by Bacillus caldotenax UvrABC endonuclease. Abstract Text:

Travis J O'Brien,Guohui Jiang,Gina Chun,H George Mandel,Craig S Westphal,Kaveh Kahen,Akbar Montaser,J Christopher States,Steven R Patierno,

Some hexavalent chromium [Cr(VI)]-containing compounds are lung carcinogens. Once within cells, Cr(VI) is reduced to trivalent chromium [Cr(III)] which displays an affinity for both DNA bases and the phosphate backbone. A diverse array of genetic lesions is produced by Cr including Cr-DNA monoadducts, DNA interstrand crosslinks (ICLs), DNA-Cr-protein crosslinks (DPCs), abasic sites, DNA strand breaks and oxidized bases. Despite the large amount of information available on the genotoxicity of Cr, little is known regarding the molecular mechanisms involved in the removal of these lesions from damaged DNA. Recent work indicates that nucleotide excision repair (NER) is involved in the processing of Cr-DNA adducts in human and rodent cells. In order to better understand this process at the molecular level and begin to identify the Cr-DNA adducts processed by NER, the incision of CrCl(3) [Cr(III)]-damaged plasmid DNA was studied using a thermal-resistant UvrABC NER endonuclease from Bacillus caldotenax (Bca). Treatment of plasmid DNA with Cr(III) (as CrCl(3)) increased DNA binding as a function of dose. For example, at a Cr(III) concentration of 1 microM we observed approximately 2 Cr(III)-DNA adducts per plasmid. At this same concentration of Cr(III) we found that approximately 17% of the plasmid DNA contained ICLs ( approximately 0.2 ICLs/plasmid). When plasmid DNA treated with Cr(III) (1 microM) was incubated with Bca UvrABC we observed approximately 0.8 incisions/plasmid. The formation of endonuclease IV-sensitive abasic lesions or Fpg-sensitive oxidized DNA bases was not detected suggesting that the incision of Cr(III)-damaged plasmid DNA by UvrABC was not related to the generation of oxidized DNA damage. Taken together, our data suggest that a sub-fraction of Cr(III)-DNA adducts is recognized and processed by the prokaryotic NER machinery and that ICLs are not necessarily the sole lesions generated by Cr(III) that are substrates for NER.

Incision of trivalent chromium [Cr(III)]-induced DNA damage by Bacillus caldotenax UvrABC endonuclease. Publishing Authors By Initials

TJ O'Brien,G Jiang,G Chun,HG Mandel,CS Westphal,K Kahen,A Montaser,JC States,SR Patierno,

For similar genetic structures: plasmids research abstracts see: genetic structures: plasmids research

PUBMED ID PMID:

MEDLINE DATE:

Incision of trivalent chromium [Cr(III)]-induced DNA damage by Bacillus caldotenax UvrABC endonuclease. Journal Published:

PUBLICATION TYPE: Research Support, U.S. Gov't,

Journal: Mutation research

VOLUME: 610

Page Numbers: 85-92

Journal Abbreviation: Mutat. Res.

ISSN: 0027-5107

DAY: 4

MONTH: 08

YEAR: 2006

Incision of trivalent chromium [Cr(III)]-induced DNA damage by Bacillus caldotenax UvrABC endonuclease. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 400763

Incision of trivalent chromium [Cr(III)]-induced DNA damage by Bacillus caldotenax UvrABC endonuclease. Keywords Mesh Terms:

KEYWORDS: Plasmids

MESH TERMS: metabolism

Chemical & Substance for Abstract: Incision of trivalent chromium [Cr(III)]-induced DNA damage by Bacillus caldotenax UvrABC endonuclease. Information

Substance Name: endodeoxyribonuclease uvrABC

Registry Number: EC 3.1.25.-

Grant and Affiliation Information for Incision of trivalent chromium [Cr(III)]-induced DNA damage by Bacillus caldotenax UvrABC endonuclease.

AFFILIATION: Department of Pharmacology and Physiology, The George Washington University, Washington, DC 20037, United States. phmtjo@gwumc.edu

Country: Netherlands

AGENCY: United States NIEHS

GRANT: ES-09961

ACRONYM: ES

MEDLINETA: Mutat Res

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Incision of trivalent chromium CrIII-induced DNA damage by Bacillus caldotenax UvrABC endonuclease Related Publications

• Excision repair is required for genotoxin-induced mutagenesis in mammalian cells.
• FANCD2 monoubiquitination and activation by hexavalent chromium [Cr(VI)] exposure: activation is not required for repair of Cr(VI)-induced DSBs.
• Incision of trivalent chromium [Cr(III)]-induced DNA damage by Bacillus caldotenax UvrABC endonuclease.