In vivo tracking of superparamagnetic iron oxide nanoparticle-labeled mesenchymal stem cell tropism to malignant gliomas using magnetic resonance imaging.

In vivo tracking of superparamagnetic iron oxide nanoparticle-labeled mesenchymal stem cell tropism to malignant gliomas using magnetic resonance imaging. Research Abstract Details 

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In vivo tracking of superparamagnetic iron oxide nanoparticle-labeled mesenchymal stem cell tropism to malignant gliomas using magnetic resonance imaging. Abstract Text:

Xing Wu,Jin Hu,Liangfu Zhou,Ying Mao,Bojie Yang,Liang Gao,Rong Xie,Feng Xu,Dong Zhang,Jun Liu,Jianhong Zhu,Xing Wu,Jin Hu,Liangfu Zhou,Ying Mao,Bojie Yang,Liang Gao,Rong Xie,Feng Xu,Dong Zhang,Jun Liu,Jianhong Zhu,

Object Mesenchymal stem cells (MSCs) have been shown to migrate toward tumors, but their distribution pattern in gliomas has not been completely portrayed. The primary purpose of the study was to assay the tropism capacity of MSCs to gliomas, to delineate the pattern of MSC distribution in gliomas after systemic injection, and to track the migration and incorporation of magnetically labeled MSCs using 1.5-T magnetic resonance (MR) imaging. Methods The MSCs from Fischer 344 rats were colabeled with superparamagnetic iron oxide nanoparticles (SPIO) and enhanced green fluorescent protein (EGFP). The tropism capacity of MSCs was quantitatively assayed in vitro using the Transwell system. To track the migration of MSCs in vivo, MR imaging was performed both 7 and 14 days after systemic administration of labeled MSCs. After MR imaging, the distribution patterns of MSCs in rats with gliomas were examined using Prussian blue and fluorescence staining. Results The in vitro study showed that MSCs possessed significantly greater migratory capacity than fibroblast cells (p < 0.001) and that lysis of F98 glioma cells and cultured F98 cells showed a greater capacity to induce migration of cells than other stimuli (p < 0.05). Seven days after MSC transplantation, the SPIO-EGFP colabeled cells were distributed throughout the tumor, where a well-defined dark hypointense region was represented on gradient echo sequences. After 14 days, most of the colabeled MSCs were found at the border between the tumor and normal parenchyma, which was represented on gradient echo sequences as diluted amorphous dark areas at the edge of the tumors. Conclusions This study demonstrated that systemically transplanted MSCs migrate toward gliomas with high specificity in a temporal-spatial pattern, which can be tracked using MR imaging.

In vivo tracking of superparamagnetic iron oxide nanoparticle-labeled mesenchymal stem cell tropism to malignant gliomas using magnetic resonance imaging. Publishing Authors By Initials

X Wu,J Hu,L Zhou,Y Mao,B Yang,L Gao,R Xie,F Xu,D Zhang,J Liu,J Zhu,X Wu,J Hu,L Zhou,Y Mao,B Yang,L Gao,R Xie,F Xu,D Zhang,J Liu,J Zhu,

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In vivo tracking of superparamagnetic iron oxide nanoparticle-labeled mesenchymal stem cell tropism to malignant gliomas using magnetic resonance imaging. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Journal of neurosurgery

VOLUME: 108

Page Numbers: 320-9

Journal Abbreviation: J. Neurosurg.

ISSN: 0022-3085

DAY: 4

MONTH: Feb

YEAR: 2008

In vivo tracking of superparamagnetic iron oxide nanoparticle-labeled mesenchymal stem cell tropism to malignant gliomas using magnetic resonance imaging. Information

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LANGUAGE: eng

NlmUniqueID: 253357

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Grant and Affiliation Information for In vivo tracking of superparamagnetic iron oxide nanoparticle-labeled mesenchymal stem cell tropism to malignant gliomas using magnetic resonance imaging.

AFFILIATION: 1 Departments of Neurosurgery and, 3 Radiology, Huashan Hospital, Fudan University; and, 2 Department of Neurosurgery, Shanghai Sixth Hospital, Jiaotong University, Shanghai, China.

Country: United States

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MEDLINETA: J Neurosurg

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