In vivo immunofluorescence to diagnose herpes simplex virus keratitis in mice.

In vivo immunofluorescence to diagnose herpes simplex virus keratitis in mice. Research Abstract Details 

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In vivo immunofluorescence to diagnose herpes simplex virus keratitis in mice. Abstract Text:

BACKGROUND/AIMS: Herpes simplex virus keratitis (HSK) is the most common cause of corneal blindness in the Western world. Delay in the treatment of HSK can lead to a more significant corneal scar and topical steroid treatment in unsuspected active HSK can lead to corneal melting. Current culture techniques for herpes simplex virus (HSV) take several days and commercially available HSV laboratory based diagnostic techniques such as Herpchek vary in sensitivity. This study was conducted to assess the viability of a new, quicker, and simpler method to diagnose HSK. METHODS: Direct immunofluorescence was used in vivo in a masked study to diagnose HSK in mice using a standard slit lamp with cobalt blue illumination. Murine monoclonal fluorescently labelled antibody was applied to the cornea for 10 or 20 minutes and then washed off with phosphate buffered solution. Mice with HSK were stained with either fluorescently labelled monoclonal antibody against HSV or fluorescently labelled monoclonal antibody against cytomegalovirus. Mice with corneal abrasions of non-viral origin were given fluorescently labelled monoclonal antibody against HSV. RESULTS: Fluorescence was seen only in the mice with HSK given fluorescently labelled monoclonal antibody against HSV. This observation was confirmed upon microscopic immunofluorescent imaging of the corneal epithelial sheets. CONCLUSION: In vivo immunofluorescence may be useful in the clinical diagnosis of HSK.

In vivo immunofluorescence to diagnose herpes simplex virus keratitis in mice. Publishing Authors By Initials

For similar animals: animal population groups: animals, inbred strains: mice, inbred strains research abstracts see: animals: animal population groups: animals, inbred strains: mice, inbred strains research

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In vivo immunofluorescence to diagnose herpes simplex virus keratitis in mice. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: The British journal of ophthalmology

VOLUME: 81

Page Numbers: 785-8

Journal Abbreviation: Br J Ophthalmol

ISSN: 0007-1161

DAY: 28

MONTH: Sep

YEAR: 1997

In vivo immunofluorescence to diagnose herpes simplex virus keratitis in mice. Information

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LANGUAGE: eng

NlmUniqueID: 421041

In vivo immunofluorescence to diagnose herpes simplex virus keratitis in mice. Keywords Mesh Terms:

KEYWORDS: Mice, Inbred Strains

MESH TERMS: diagnosis

Chemical & Substance for Abstract: In vivo immunofluorescence to diagnose herpes simplex virus keratitis in mice. Information

Substance Name: Antibodies, Monoclonal

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Grant and Affiliation Information for In vivo immunofluorescence to diagnose herpes simplex virus keratitis in mice.

AFFILIATION: Department of Medical Microbiology, University of Wales College of Medicine, Cardiff.

Country: ENGLAND

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MEDLINETA: Br J Ophthalmol

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