In vivo imaging in a murine model of glioblastoma.

In vivo imaging in a murine model of glioblastoma. Research Abstract Details 

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In vivo imaging in a murine model of glioblastoma. Abstract Text:

Sarah C Jost,John E Wanebo,Sheng-Kwei Song,Michael R Chicoine,Keith M Rich,Thomas A Woolsey,Jason S Lewis,Robert H Mach,Jinbin Xu,Joel R Garbow,

OBJECTIVE: To use in vivo imaging methods in mice to quantify intracranial glioma growth, to correlate images and histopathological findings, to explore tumor marker specificity, to assess effects on cortical function, and to monitor effects of chemotherapy. METHODS: Mice with DBT glioma cell tumors implanted intracranially were imaged serially with a 4.7-T small-animal magnetic resonance imaging (MRI) scanner. MRI tumor volumes were measured and correlated with postmortem histological findings. Different nonspecific and specific positron emission tomography radiopharmaceuticals, [18F]2-fluoro-2-deoxy-d-glucose, [18F]3'-deoxy-3'-fluorothymidine, or [11C]RHM-I, a sigma2-receptor ligand, were visualized with microPET (CTI-Concorde MicroSystems LLC, Knoxville, TN). Intrinsic optical signals were imaged serially during contralateral whisker stimulation to study the impact of tumor growth on cortical function. Other groups of mice were imaged serially with MRI after one or two doses of the antimitotic N,N'-bis(2-chloroethyl)-N-nitrosourea (BCNU). RESULTS: MRI and histological tumor volumes were highly correlated (r2 = 0.85). Significant binding of [11C]RHM-I was observed in growing tumors. Over time, tumors reduced and displaced (P # 0.001) whisker-activated intrinsic optical signals but did not change intrinsic optical signals in the contralateral hemisphere. Tumor growth was delayed 7 days after a single dose of BCNU and 18 days after two doses of BCNU. Mean tumor volume 15 days after DBT implantation was significantly smaller for treated mice (1- and 2-dose BCNU) compared with controls (P = 0.0026). CONCLUSION: Mouse MRI, positron emission tomography, and optical imaging provide quantitative and qualitative in vivo assessments of intracranial tumors that correlate directly with tumor histological findings. The combined imaging approach provides powerful multimodality assessments of tumor progression, effects on brain function, and responses to therapy.

In vivo imaging in a murine model of glioblastoma. Publishing Authors By Initials

SC Jost,JE Wanebo,SK Song,MR Chicoine,KM Rich,TA Woolsey,JS Lewis,RH Mach,J Xu,JR Garbow,

For similar xenograft model antitumor assays research abstracts see: xenograft model antitumor assays research

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In vivo imaging in a murine model of glioblastoma. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: Neurosurgery

VOLUME: 60

Page Numbers: 360-70; discussion 370-1

Journal Abbreviation: Neurosurgery

ISSN: 1524-4040

DAY: 3

MONTH: Feb

YEAR: 2007

In vivo imaging in a murine model of glioblastoma. Information

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LANGUAGE: eng

NlmUniqueID: 7802914

In vivo imaging in a murine model of glioblastoma. Keywords Mesh Terms:

KEYWORDS: Xenograft Model Antitumor Assays

MESH TERMS: methods

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Grant and Affiliation Information for In vivo imaging in a murine model of glioblastoma.

AFFILIATION: Department of Neurosurgery, Washington University, School of Medicine, St. Louis, Missouri 63110, USA.

Country: United States

AGENCY: United States NCI

GRANT: R24 CA83060

ACRONYM: CA

MEDLINETA: Neurosurgery

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