In vivo functional characterization of the Saccharomyces cerevisiae 60S biogenesis GTPase Nog1.

In vivo functional characterization of the Saccharomyces cerevisiae 60S biogenesis GTPase Nog1. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

In vivo functional characterization of the Saccharomyces cerevisiae 60S biogenesis GTPase Nog1. Abstract Text:

Jennifer L Fuentes,Kaustuv Datta,Susan M Sullivan,Angela Walker,Janine R Maddock,

The Saccharomyces cerevisiae Nog1 GTPase is critical for assembly of the large ribosomal subunit. Mutations in conserved residues in the GTP-binding pocket cause defects in cell growth and 60S ribosome assembly but mutant proteins retain their ability to associate with the pre-60S. Association of Nog1 with the pre-60S is independent of guanine nucleotide added to cell extracts. Thus, it appears that nucleotide occupancy does not substantially affect Nog1 association with pre-60S particles. Somewhat surprisingly, neither of the conserved threonines in the G2 motif of the GTPase domain is essential for Nog1 function. Neither the steady-state rRNA levels nor the protein composition (as determined by isobaric labeling and identification by mass spectrometry of peptides) of the pre-60S particles in the nog1P176V mutant are grossly perturbed, although levels of four proteins (Nog1, Nop2, Nop15, and Tif6) are modestly reduced in pre-60S particles isolated from the mutant. Deletion analysis revealed that the C-terminal 168 amino acids are not required for function; however, the N-terminal 126 amino acids are required. Optimal association with pre-60S particles requires sequences between amino acids 347-456. Several conserved charge-to-alanine substitutions outside the GTPase domain display modest growth phenotypes indicating that these residues are not critical for function.

In vivo functional characterization of the Saccharomyces cerevisiae 60S biogenesis GTPase Nog1. Publishing Authors By Initials

JL Fuentes,K Datta,SM Sullivan,A Walker,JR Maddock,

For similar biochemical phenomena, metabolism, and nutrition: biochemical phenomena: sequence homology: sequence homology, amino acid research abstracts see: biochemical phenomena, metabolism, and nutrition: biochemical phenomena: sequence homology: sequence homology, amino acid research

PUBMED ID PMID:

MEDLINE DATE:

In vivo functional characterization of the Saccharomyces cerevisiae 60S biogenesis GTPase Nog1. Journal Published:

PUBLICATION TYPE: Research Support, U.S. Gov't,

Journal: Molecular genetics and genomics : MGG

VOLUME: 278

Page Numbers: 105-23

Journal Abbreviation: Mol. Genet. Genomics

ISSN: 1617-4615

DAY: 19

MONTH: 04

YEAR: 2007

In vivo functional characterization of the Saccharomyces cerevisiae 60S biogenesis GTPase Nog1. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 101093320

In vivo functional characterization of the Saccharomyces cerevisiae 60S biogenesis GTPase Nog1. Keywords Mesh Terms:

KEYWORDS: Sequence Homology, Amino Acid

MESH TERMS: metabolism

Chemical & Substance for Abstract: In vivo functional characterization of the Saccharomyces cerevisiae 60S biogenesis GTPase Nog1. Information

Substance Name: GTP-Binding Proteins

Registry Number: EC 3.6.1.-

Grant and Affiliation Information for In vivo functional characterization of the Saccharomyces cerevisiae 60S biogenesis GTPase Nog1.

AFFILIATION: Department of Molecular, Cellular and Developmental Biology, University of Michigan, 830 North University Avenue, Ann Arbor, MI 48109, USA.

Country: Germany

AGENCY: United States NIGMS

GRANT: GM-55133-S1

ACRONYM: GM

MEDLINETA: Mol Genet Genomics

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

In vivo functional characterization of the Saccharomyces cerevisiae 60S biogenesis GTPase Nog1 Related Publications

• A second group off shell elements.
• A survey of policies and local ordinances supporting physical activity in hawaii counties.
• Ampicillin in the treatment of typhoid fever.
• Antihormone formation complicating pituitary gonadotropin therapy in infertile men, properties of the antihormones.
• Antihormone formation complicating pituitary gonadotropin therapy in infertile men; effect on number of sperm, morphology of the testis and urinary gonadotropins.
• Biological properties of acetylcysteine: assay development and pharmacokinetic studies.
• COLONEL E. C. G. Maddock.
• Carcinoma of the rectum; treatment with preservation of the sphincters.
• EDWARD Cecil Gordon Maddock.
• Early events of Bacillus anthracis germination identified by time-course quantitative proteomics.
• Environmental factors of obesity in communities with native Hawaiians.
• Etiology of acute pancreatitis; an experimental study.
• Fluorides of the natural radioactive elements.
• Function of the postmenopausal ovary; comparison of urinary estrogen and gonadotropin excretion and response to administration of FSH in postmenopausal and ovariectomized women.
• Hypervitaminosis A in the dog.
• Identification of novel Escherichia coli ribosome-associated proteins using isobaric tags and multidimensional protein identification techniques.
• In vivo functional characterization of the Saccharomyces cerevisiae 60S biogenesis GTPase Nog1.
• Lactic acidosis complicating treatment of ketosis of labour.
• Observations on air swallowing during operations.
• Parenteral use of water, dextrose, electrolytes, fat and vitamins.
• Polar explorations Recent insights into the polarity of bacterial proteins.
• Some fundamentals in water and electrolyte balance.
• Some observations on gastro-intestinal distention.
• Submucous lipoma of the stomach; report of a case.
• The incidence of head lice in 1947 compared with 1938-40.
• The treatment of carcinoma of the midrectum with preservation of the sphincters.
• Time course of the subjective emotional response to aversive pictures: relevance to fMRI studies.
• Validity of beachgoers' self-report of their sun habits.
• Very low birth weight children at school age: comparison of neonatal management methods.
• [Not Available.]