[In vivo endochondral bone formation by implanting human bone morphogenetic protein-2-producing fibroblasts into nude mouse muscle]

[In vivo endochondral bone formation by implanting human bone morphogenetic protein-2-producing fibroblasts into nude mouse muscle] Research Abstract Details 

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[In vivo endochondral bone formation by implanting human bone morphogenetic protein-2-producing fibroblasts into nude mouse muscle] Abstract Text:

Xiang-dong Li,Yun-yu Hu,

OBJECTIVE: To determine whether fibroblasts can be used to promote endochondral bone formation in vivo by transfer of human bone morphogenetic protein-2(hBMP-2) into fibroblasts. METHODS: pcDNA3-hBMP-2 was constructed by use of gene clone and recombined technique. NIH3T3 fibroblasts were transfected with pcDNA3-hBMP-2. The positive cell clones were selected with G418. In NIH3T3 fibroblasts transferred with pcDNA3-hBMP-2, the expression of hBMP-2 was determined by in situ hybridization and immunohistochemical analysis; alkaline phosphatase activity was measured. hBMP-2-producing fibroblasts were implanted into nude mouse muscle to observe endochondral bone formation in vivo. RESULTS: pcDNA3-hBMP-2 was successfully constructed. In NIH3T3 fibroblasts transfected with pcDNA3-hBMP-2, the BMP-2 expression was stable; alkaline phophatase activity was much higher than that in non-transfected NIH3T3 cells. Endochondral bone formation in vivo was observed at the site of implantation 4 weeks later. CONCLUSION: Fibroblasts transfected by hBMP-2 gene can be used to promote endochondral bone formation in vivo.

[In vivo endochondral bone formation by implanting human bone morphogenetic protein-2-producing fibroblasts into nude mouse muscle] Publishing Authors By Initials

XD Li,YY Hu,

For similar peptides: intercellular signaling peptides and proteins: cytokines: transforming growth factor beta research abstracts see: peptides: intercellular signaling peptides and proteins: cytokines: transforming growth factor beta research

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[In vivo endochondral bone formation by implanting human bone morphogenetic protein-2-producing fibroblasts into nude mouse muscle] Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Zhongguo xiu fu chong jian wai ke za zhi = Zhonggu

VOLUME: 17

Page Numbers: 177-9

Journal Abbreviation:

ISSN: 1002-1892

DAY: 19

MONTH: May

YEAR: 2003

[In vivo endochondral bone formation by implanting human bone morphogenetic protein-2-producing fibroblasts into nude mouse muscle] Information

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LANGUAGE: chi

NlmUniqueID: 9425194

[In vivo endochondral bone formation by implanting human bone morphogenetic protein-2-producing fibroblasts into nude mouse muscle] Keywords Mesh Terms:

KEYWORDS: Transforming Growth Factor beta

MESH TERMS: physiology

Chemical & Substance for Abstract: [In vivo endochondral bone formation by implanting human bone morphogenetic protein-2-producing fibroblasts into nude mouse muscle] Information

Substance Name: bone morphogenetic protein 2

Registry Number: 0

Grant and Affiliation Information for [In vivo endochondral bone formation by implanting human bone morphogenetic protein-2-producing fibroblasts into nude mouse muscle]

AFFILIATION: Institute of Orthopedics, Xijing Hospital, Fourth Military Medical University, Xi'an Shaanxi, P. R. China 710032.

Country: China

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MEDLINETA: Zhongguo Xiu Fu Chong Jian Wai

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