In vivo dysfunction of the term alveolar macrophage after in utero ethanol exposure.

In vivo dysfunction of the term alveolar macrophage after in utero ethanol exposure. Research Abstract Details 

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In vivo dysfunction of the term alveolar macrophage after in utero ethanol exposure. Abstract Text:

Xiao-Du Ping,Frank L Harris,Lou Ann S Brown,Theresa W Gauthier,

BACKGROUND: The effects of in utero alcohol exposure on the immune function of the newborn remain under investigation. Fetal ethanol (ETOH) exposure increases oxidative stress in the developing lung, in part due to decreased availability of the antioxidant glutathione (GSH). We have previously shown that in utero ETOH impairs alveolar macrophage phagocytosis and viability in the premature pup, while maintaining GSH availability with maternal supplementation of S-adenosyl-methionine (SAM) during ETOH ingestion improves macrophage function and viability. We hypothesized that dysfunction of the neonatal alveolar macrophage exposed to ETOH in utero would persist at term gestation. METHODS: Using a guinea-pig model of fetal ETOH exposure, timed-pregnant guinea-pigs were pair-fed ETOH+/-the GSH precursor SAM and the diet continued until spontaneous delivery. Term alveolar macrophages were evaluated using fluorescent microscopy for phagocytosis and apoptosis after in vitro incubation with Staphalococcus aureus. Using an in vivo model of intranasal Staph. aureus inoculation, the in vivo function of the term alveolar macrophage was also investigated using confocal fluorescent analysis. RESULTS: In utero ETOH exposure increased oxidant stress in the alveolar macrophage and decreased phagocytosis and viability in vitro and in vivo. Confocal analysis of phagocytosis in vivo demonstrated a marked impairment of internalization of the bacteria by the ETOH-exposed alveolar macrophage. The addition of SAM during maternal ETOH ingestion prevented loss of alveolar macrophage function and viability in vitro and in vivo. CONCLUSIONS: In utero ETOH exposure impairs alveolar macrophage function and viability in vitro and in vivo even at term gestation. The ETOH-induced changes in macrophage function and viability can be ablated with maternal SAM supplementation. Further investigations are required to identify the mechanisms of ETOH-induced derangement of phagocytosis in the neonatal alveolar macrophage and the clinical ramifications of altered immune function after in utero alcohol exposure for the newborn.

In vivo dysfunction of the term alveolar macrophage after in utero ethanol exposure. Publishing Authors By Initials

XD Ping,FL Harris,LA Brown,TW Gauthier,

For similar bacteria: gram-positive bacteria: gram-positive cocci: staphylococcaceae: staphylococcus: staphylococcus aureus research abstracts see: bacteria: gram-positive bacteria: gram-positive cocci: staphylococcaceae: staphylococcus: staphylococcus aureus research

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MEDLINE DATE:

In vivo dysfunction of the term alveolar macrophage after in utero ethanol exposure. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: Alcoholism, clinical and experimental research

VOLUME: 31

Page Numbers: 308-16

Journal Abbreviation: Alcohol. Clin. Exp. Res.

ISSN: 0145-6008

DAY: 3

MONTH: Feb

YEAR: 2007

In vivo dysfunction of the term alveolar macrophage after in utero ethanol exposure. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 7707242

In vivo dysfunction of the term alveolar macrophage after in utero ethanol exposure. Keywords Mesh Terms:

KEYWORDS: Staphylococcus aureus

MESH TERMS: metabolism

Chemical & Substance for Abstract: In vivo dysfunction of the term alveolar macrophage after in utero ethanol exposure. Information

Substance Name: Glutathione

Registry Number: 70-18-8

Grant and Affiliation Information for In vivo dysfunction of the term alveolar macrophage after in utero ethanol exposure.

AFFILIATION: Department of Pediatrics, Division of Neonatal-Perinatal Medicine, Emory University, Atlanta, Georgia 30322, USA.

Country: England

AGENCY: United States NIAAA

GRANT: R01 AA013979

ACRONYM: AA

MEDLINETA: Alcohol Clin Exp Res

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