In vivo beta-defensin gene expression in rat gingival epithelium in response to Actinobacillus actinomycetemcomitans infection.

In vivo beta-defensin gene expression in rat gingival epithelium in response to Actinobacillus actinomycetemcomitans infection. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

In vivo beta-defensin gene expression in rat gingival epithelium in response to Actinobacillus actinomycetemcomitans infection. Abstract Text:

A R Kurland,H Schreiner,G Diamond,

BACKGROUND AND OBJECTIVE: Human beta-defensins have been identified in the oral cavity and are predicted to play a role in the defense against pathogenic bacteria. Homologous rat beta-defensins (RBDs) have been identified, but their expression in the oral cavity has not been examined. Therefore, the aim of this study was to investigate the expression of innate immune mediators in the rat gingival epithelium. MATERIAL AND METHODS: Rats were pretreated with antibiotics to depress the normal oral flora, followed by the introduction of Actinobacillus actinomycetemcomitans in their food to allow colonization and the development of periodontal disease. At various time points, animals were killed and the gingival epithelium was extracted. Semiquantitative reverse transcription-polymerase chain reaction was performed to measure RBD and Toll-like receptor (TLR) mRNA levels. RESULTS: Three beta-defensins (RBD-1, -2 and -5) and two TLRs (TLR-3 and -4) are expressed in normal rat gingival epithelium. After the introduction of A. actinomycetemcomitans, RBD-1 and RBD-2 mRNA levels increased for the first week followed by a return to basal levels. No change in TLR mRNA levels was observed. CONCLUSION: The rat model provides a good system for experimental analysis of the innate immune response to periopathogenic bacteria in the oral cavity, as well as the potential role of beta-defensins in the host response to colonization.

In vivo beta-defensin gene expression in rat gingival epithelium in response to Actinobacillus actinomycetemcomitans infection. Publishing Authors By Initials

AR Kurland,H Schreiner,G Diamond,

For similar proteins: membrane proteins: receptors, cell surface: receptors, immunologic: receptors, pattern recognition: toll-like receptors research abstracts see: proteins: membrane proteins: receptors, cell surface: receptors, immunologic: receptors, pattern recognition: toll-like receptors research

PUBMED ID PMID:

MEDLINE DATE:

In vivo beta-defensin gene expression in rat gingival epithelium in response to Actinobacillus actinomycetemcomitans infection. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Journal of periodontal research

VOLUME: 41

Page Numbers: 567-72

Journal Abbreviation: J. Periodont. Res.

ISSN: 0022-3484

DAY: 3

MONTH: Dec

YEAR: 2006

In vivo beta-defensin gene expression in rat gingival epithelium in response to Actinobacillus actinomycetemcomitans infection. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 55107

In vivo beta-defensin gene expression in rat gingival epithelium in response to Actinobacillus actinomycetemcomitans infection. Keywords Mesh Terms:

KEYWORDS: Toll-Like Receptors

MESH TERMS: genetics

Chemical & Substance for Abstract: In vivo beta-defensin gene expression in rat gingival epithelium in response to Actinobacillus actinomycetemcomitans infection. Information

Substance Name: beta defensin-1 protein, rat

Registry Number: 0

Grant and Affiliation Information for In vivo beta-defensin gene expression in rat gingival epithelium in response to Actinobacillus actinomycetemcomitans infection.

AFFILIATION: Department of Oral Biology, UMDNJ-New Jersey Dental School, Newark, NJ 07101, USA.

Country: Denmark

AGENCY: United States NIDCR

GRANT: R01DE14897S1

ACRONYM: DE

MEDLINETA: J Periodontal Res

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

In vivo beta-defensin gene expression in rat gingival epithelium in response to Actinobacillus actinomycetemcomitans infection Related Publications

• A word to the wise: Tips for the first-time traveler.
• Ambient manganese exposure is negatively associated with human sperm motility and concentration.
• Antibody recognition and neutralization determinants on domains I and II of West Nile Virus envelope protein.
• Antibody recognition of cell surface-associated NS1 triggers Fc-gamma receptor-mediated phagocytosis and clearance of West Nile Virus-infected cells.
• Antigen-specific cytotoxic T lymphocytes protect against lethal West Nile virus encephalitis.
• Biotinylated photocleavable polyethylenimine: capture and triggered release of nucleic acids from solid supports.
• CD4+ T-cell responses are required for clearance of West Nile virus from the central nervous system.
• CD40-CD40 ligand interactions promote trafficking of CD8+ T cells into the brain and protection against West Nile virus encephalitis.
• Caspase 3-dependent cell death of neurons contributes to the pathogenesis of West Nile virus encephalitis.
• Cell-specific IRF-3 responses protect against West Nile virus infection by interferon-dependent and -independent mechanisms.
• Consequences of variations in genes that affect dopamine in prefrontal cortex.
• Defining limits of treatment with humanized neutralizing monoclonal antibody for West Nile virus neurological infection in a hamster model.
• Enhanced discrimination of single nucleotide polymorphism in genotyping by phosphorothioate proofreading allele-specific amplification.
• Functional comparison of T cells recognizing cytomegalovirus pp65 and intermediate-early antigen polypeptides in hematopoietic stem-cell transplant and solid organ transplant recipients.
• Humanized monoclonal antibody against West Nile virus envelope protein administered after neuronal infection protects against lethal encephalitis in hamsters.
• In vivo beta-defensin gene expression in rat gingival epithelium in response to Actinobacillus actinomycetemcomitans infection.
• Interrelated and interdependent.
• Longitudinal assessment of cytomegalovirus (CMV)-specific immune responses in liver transplant recipients at high risk for late CMV disease.
• Matrix protein microarrays for spatially and compositionally controlled microspot thrombosis under laminar flow.
• Methods for mapping protease specificity.
• Microinfusion of pituitary adenylate cyclase-activating polypeptide into the central nucleus of amygdala of the rat produces a shift from an active to passive mode of coping in the shock-probe fear/defensive burying test.
• Novel integrated paired emitter-detector diode (PEDD) as a miniaturized photometric detector in HPLC.
• Selenium and GPx-1 overexpression protect mammalian cells against UV-induced DNA damage.
• Synthesis and structure-activity relationships of a series of increasingly hydrophobic cationic steroid lipofection reagents.
• Systemic lupus erythematosus and Sjögren's syndrome.
• The molecular basis of antibody-mediated neutralization of West Nile virus.
• The relationship of antipsychotic medication class and adherence with treatment outcomes and costs for Florida Medicaid beneficiaries with schizophrenia.
• Thresholds in songbird occurrence in relation to landscape structure.
• Time and recruitment costs as currencies in manipulation studies on the costs of reproduction.
• West Nile virus nonstructural protein NS1 inhibits complement activation by binding the regulatory protein factor H.