In Vivo beta-adrenergic activation of atrial natriuretic factor (ANF) reporter expression.

In Vivo beta-adrenergic activation of atrial natriuretic factor (ANF) reporter expression. Research Abstract Details 

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In Vivo beta-adrenergic activation of atrial natriuretic factor (ANF) reporter expression. Abstract Text:

J G Edwards,

Isoproterenol (ISO) infusion increases ANF-mRNA levels and control of ANF expression lies at the level of transcription. In neonatal cardiomyocytes, previous investigations determined that the -125 to -100 region of the rat ANF 5' flanking region contained cis-elements critical for control of ISO induced ANF transcription. However, it is unclear if these same cis-elements regulate ANF transcription in vivo. To examine this question, reporter plasmids containing the ANF 5' flanking/promoter region were injected directly into the left ventricle. Following a recovery period, osmotic pumps were implanted to infuse vehicle or ISO (0.2 or 2.0 mg/kg/d). ISO significantly (p < .05) increased the LV/BW ratio in a dose dependent, but not a time dependent manner. ISO significantly (p < .05) increased ANF reporter expression in both a dose-dependent and time dependent manner. Injections into the midwall of the LV or into the apex did not lead to significant differences in ISO-induced ANF reporter expression. Using site-specific mutations of ANF reporter constructs, comparisons were made of ISO induced ANF transcription in vitro in neonatal cardiomyocytes and in vivo in the adult heart. Cis-elements critical for ISO activation in cultured cardiomyocytes were not essential for the increased expression of the ANF reporters in vivo. The results indicate that distinct differences in ANF transcriptional regulation exist in vivo in the adult heart as compared with neonatal cardiomyocytes, and suggest the recruitment of other signaling pathways beyond adrenergic-receptor mediated pathways.

In Vivo beta-adrenergic activation of atrial natriuretic factor (ANF) reporter expression. Publishing Authors By Initials

JG Edwards,

For similar proteins: membrane proteins: receptors, cell surface: receptors, biogenic amine: receptors, catecholamine: receptors, adrenergic: receptors, adrenergic, beta research abstracts see: proteins: membrane proteins: receptors, cell surface: receptors, biogenic amine: receptors, catecholamine: receptors, adrenergic: receptors, adrenergic, beta research

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In Vivo beta-adrenergic activation of atrial natriuretic factor (ANF) reporter expression. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: Molecular and cellular biochemistry

VOLUME: 292

Page Numbers: 119-29

Journal Abbreviation: Mol. Cell. Biochem.

ISSN: 0300-8177

DAY: 15

MONTH: 08

YEAR: 2006

In Vivo beta-adrenergic activation of atrial natriuretic factor (ANF) reporter expression. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 364456

In Vivo beta-adrenergic activation of atrial natriuretic factor (ANF) reporter expression. Keywords Mesh Terms:

KEYWORDS: Receptors, Adrenergic, beta

MESH TERMS: metabolism

Chemical & Substance for Abstract: In Vivo beta-adrenergic activation of atrial natriuretic factor (ANF) reporter expression. Information

Substance Name: Atrial Natriuretic Factor

Registry Number: 85637-73-6

Grant and Affiliation Information for In Vivo beta-adrenergic activation of atrial natriuretic factor (ANF) reporter expression.

AFFILIATION: Department of Physiology, New York Medical College, Valhalla, New York, USA. jgedwards@nymc.edu

Country: Netherlands

AGENCY: United States NHLBI

GRANT: HL59417

ACRONYM: HL

MEDLINETA: Mol Cell Biochem

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