In vitro splicing of a chicken delta-crystallin pre-mRNA in a mammalian nuclear extract.

In vitro splicing of a chicken delta-crystallin pre-mRNA in a mammalian nuclear extract. Research Abstract Details 

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In vitro splicing of a chicken delta-crystallin pre-mRNA in a mammalian nuclear extract. Abstract Text:

H Sakamoto,M Ohno,K Yasuda,K Mizumoto,Y Shimura,

An in vitro splicing system was constructed using portions of chicken delta-crystallin pre-mRNA synthesized in vitro and a HeLa nuclear extract. Analysis of the reaction products revealed that about 25% of the pre-mRNA was precisely spliced at 30 degrees C in 2 h under the standard conditions. The other major products of the reaction detected were a 5'-exon fragment and three RNA species showing unusual electrophoretic mobilities on polyacrylamide gels. Structural analyses showed that these three RNAs contain a branch (lariat) structure as seen in the in vitro splicing reactions of human beta-globin, adenovirus, and yeast pre-mRNAs. In addition, methylation at the N-7 position of the blocking guanosine of the 5'-terminal cap structure of pre-mRNA has been suggested to play an important role in the splicing reaction.

In vitro splicing of a chicken delta-crystallin pre-mRNA in a mammalian nuclear extract. Publishing Authors By Initials

H Sakamoto,M Ohno,K Yasuda,K Mizumoto,Y Shimura,

For similar genetic processes: gene expression: transcription, genetic research abstracts see: genetic processes: gene expression: transcription, genetic research

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In vitro splicing of a chicken delta-crystallin pre-mRNA in a mammalian nuclear extract. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Journal of biochemistry

VOLUME: 102

Page Numbers: 1289-301

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: Nov

YEAR: 1987

In vitro splicing of a chicken delta-crystallin pre-mRNA in a mammalian nuclear extract. Information

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LANGUAGE: eng

NlmUniqueID: 376600

In vitro splicing of a chicken delta-crystallin pre-mRNA in a mammalian nuclear extract. Keywords Mesh Terms:

KEYWORDS: Transcription, Genetic

MESH TERMS: genetics

Chemical & Substance for Abstract: In vitro splicing of a chicken delta-crystallin pre-mRNA in a mammalian nuclear extract. Information

Substance Name: DNA

Registry Number: 9007-49-2

Grant and Affiliation Information for In vitro splicing of a chicken delta-crystallin pre-mRNA in a mammalian nuclear extract.

AFFILIATION: Department of Biophysics, Faculty of Science, Kyoto University.

Country: JAPAN

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MEDLINETA: J Biochem

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