In vitro sequence-specific cleavage in transcribed spacer of mouse precursor ribosomal RNA.

In vitro sequence-specific cleavage in transcribed spacer of mouse precursor ribosomal RNA. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

In vitro sequence-specific cleavage in transcribed spacer of mouse precursor ribosomal RNA. Abstract Text:

M Nashimoto,K Ogata,Y Mishima,

When we compared the sequences near four terminal sites of transcripts including two endoribonucleolytic cleavage sites in external transcribed spacer 1 upstream of the 5' end of the mouse 18S rRNA (Mishima, Y., Mitsuma, T., & Ogata, K. (1985) EMBO J. 4, 3879-3886), a seven-nucleotide consensus sequence, GGPyUUGPy (Py is C or U), was obtained. The results of both in vitro pulse-chase experiments and S1 nuclease mappings using the mouse rDNA fragment of the transcription initiation region indicated that ribonucleolytic cleavages take place in the sequence matching the consensus sequence at more than five nucleotides out of the seven positions. Furthermore, effective ribonucleolytic cleavages in vitro were observed in a sequence, GGCUUGU, in the internal transcribed spacer 2 located between 5.8S and 28S rRNA. These results demonstrate that the ribonucleolytic cleavages occur preferentially in the sequence of GGPyUUGPy in the transcribed spacer regions of the mouse pre-rRNA. From this information, we infer the existence of processing steps for the pre-rRNA maturation involving the sequence-specific ribonucleolytic cleavages.

In vitro sequence-specific cleavage in transcribed spacer of mouse precursor ribosomal RNA. Publishing Authors By Initials

M Nashimoto,K Ogata,Y Mishima,

For similar genetic processes: gene expression: transcription, genetic research abstracts see: genetic processes: gene expression: transcription, genetic research

PUBMED ID PMID:

MEDLINE DATE:

In vitro sequence-specific cleavage in transcribed spacer of mouse precursor ribosomal RNA. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Journal of biochemistry

VOLUME: 103

Page Numbers: 992-7

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: Jun

YEAR: 1988

In vitro sequence-specific cleavage in transcribed spacer of mouse precursor ribosomal RNA. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 376600

In vitro sequence-specific cleavage in transcribed spacer of mouse precursor ribosomal RNA. Keywords Mesh Terms:

KEYWORDS: Transcription, Genetic

MESH TERMS: genetics

Chemical & Substance for Abstract: In vitro sequence-specific cleavage in transcribed spacer of mouse precursor ribosomal RNA. Information

Substance Name: DNA

Registry Number: 9007-49-2

Grant and Affiliation Information for In vitro sequence-specific cleavage in transcribed spacer of mouse precursor ribosomal RNA.

AFFILIATION: Department of Biochemistry, Niigata University School of Medicine.

Country: JAPAN

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: J Biochem

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

In vitro sequence-specific cleavage in transcribed spacer of mouse precursor ribosomal RNA Related Publications

• 4-Borono-2-[18F]fluoro-D,L-phenylalanine as a target compound for boron neutron capture therapy: tumor imaging potential with positron emission tomography.
• 4-Borono-2-[18F]fluoro-D,L-phenylalanine: a possible tracer for melanoma diagnosis with PET.
• Analysis of longitudinal changes in the psychological status of patients with asthma.
• Antimicrobial susceptibilities of Streptococcus pneumoniae isolated from adult patients with community-acquired pneumonia in Japan.
• Aqueous humour levels of cytokines are correlated to vitreous levels and severity of macular oedema in branch retinal vein occlusion.
• Atopic features of cough variant asthma and classic asthma with wheezing.
• Autologous blood injection for marked overfiltration early after trabeculectomy with mitomycin C.
• Biodegradable particle formation for drug and gene delivery using supercritical fluid and dense gas.
• Cellular blue nevus. Melanogenic activity and malignant transformation.
• Corticosteroid-induced myopathy mimicking therapy-resistant asthma.
• Dietary intake of folate, other B vitamins, and omega-3 polyunsaturated fatty acids in relation to depressive symptoms in Japanese adults.
• Double-outlet technique for tetralogy of Fallot-type disease with an anomalous coronary artery.
• Eccrine-centered nevus.
• Eye stiffness measurement by probe contact method.
• In vitro sequence-specific cleavage in transcribed spacer of mouse precursor ribosomal RNA.
• Intracoronary nicorandil relieves multiple coronary vasospasm with hemodynamic collapse.
• Morphological mechanism of the development of pulmonary emphysema in klotho mice.
• Patients' characteristics associated with unsuccessful sputum induction in asthma.
• Plasma thioredoxin, a novel oxidative stress marker, in patients with obstructive sleep apnea before and after nasal continuous positive airway pressure.
• Pneumococcal surface protein A family types of Streptococcus pneumoniae from community-acquired pneumonia patients in Japan.
• Redox-based therapeutics for lung diseases.
• Structural effects on interconversion of oxygen-substituted bisketenes and cyclobutenediones.
• Substituting latanoprost (Xalatan) for isopropyl unoprostone (Rescula) in monotherapy and combination therapy.
• Surgical repair of tetralogy of Fallot with large conus artery.
• Surgical treatment for infected thrombus in the superior vena cava using an off-pump venoatrial shunt.
• Synthesis and radiation dosimetry of 4-borono-2-[18F]fluoro-D,L-phenylalanine: a target compound for PET and boron neutron capture therapy.
• TGFB1 promoter polymorphism C-509T and pathophysiology of asthma.
• Translocation of dilated pulmonary artery for relief of bronchial compression associated with ventricular septal defect.
• Ultrasound biomicroscopy in the management of Vogt-Koyanagi-Harada disease.
• [Physiology of the cornea and contact lenses]