In vitro platelet adhesion on polymeric surfaces with varying fluxes of continuous nitric oxide release.

In vitro platelet adhesion on polymeric surfaces with varying fluxes of continuous nitric oxide release. Research Abstract Details 

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In vitro platelet adhesion on polymeric surfaces with varying fluxes of continuous nitric oxide release. Abstract Text:

Yiduo Wu,Zhengrong Zhou,Mark E Meyerhoff,

Nitric oxide (NO) is released by endothelial cells that line the inner walls of healthy blood vessels at fluxes ranging from 0.5 x 10(-10) to 4.0 x 10(-10) mol cm(-2) min(-1), and this continuous NO release contributes to the extraordinary thromboresistance of the intact endothelium. To improve the biocompatibility of blood-contacting devices, a biomimetic approach to release/generate NO at polymer/blood interfaces has been pursued recently (with NO donors or NO generating catalysts doped within polymeric coatings) and this concept has been shown to be effective in preventing platelet adhesion/activation via several in vivo animal studies. However, there are no reports to date describing any quantitative in vitro assay to evaluate the blood compatibilities of such NO release/generating polymers with controlled NO fluxes. Such a methodology is desired to provide a preliminary assessment of any new NO-releasing material, in terms of the effectiveness of given NO fluxes and NO donor amounts on platelet activity before the more complex and costly in vivo testing is carried out. In this article, we report the use of a lactate dehydrogenase assay to study in vitro platelet adhesion on such NO-releasing polymer surfaces with varying NO fluxes. Reduced platelet adhesion was found to correlate with increasing NO fluxes. The highest NO flux tested, 7.05 (+/-0.25) x 10(-10) mol cm(-2) min(-1), effectively reduced platelet adhesion to nearly 20% of its original level (from 14.0 (+/-2.1) x 10(5) cells cm(-2) to 2.96 (+/-0.18) x 10(5) cells cm(-2)) compared to the control polymer coating without NO release capability.

In vitro platelet adhesion on polymeric surfaces with varying fluxes of continuous nitric oxide release. Publishing Authors By Initials

Y Wu,Z Zhou,ME Meyerhoff,

For similar natural sciences: chemistry: chemistry, physical: surface properties research abstracts see: natural sciences: chemistry: chemistry, physical: surface properties research

PUBMED ID PMID:

MEDLINE DATE:

In vitro platelet adhesion on polymeric surfaces with varying fluxes of continuous nitric oxide release. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: Journal of biomedical materials research. Part A

VOLUME: 81

Page Numbers: 956-63

Journal Abbreviation:

ISSN: 1549-3296

DAY: 15

MONTH: Jun

YEAR: 2007

In vitro platelet adhesion on polymeric surfaces with varying fluxes of continuous nitric oxide release. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 101234237

In vitro platelet adhesion on polymeric surfaces with varying fluxes of continuous nitric oxide release. Keywords Mesh Terms:

KEYWORDS: Surface Properties

MESH TERMS: pharmacology

Chemical & Substance for Abstract: In vitro platelet adhesion on polymeric surfaces with varying fluxes of continuous nitric oxide release. Information

Substance Name: L-Lactate Dehydrogenase

Registry Number: EC 1.1.1.27

Grant and Affiliation Information for In vitro platelet adhesion on polymeric surfaces with varying fluxes of continuous nitric oxide release.

AFFILIATION: Department of Chemistry, The University of Michigan, Ann Arbor, Michigan 48109-1055, USA.

Country: United States

AGENCY: United States NICHD

GRANT: HD-015434

ACRONYM: HD

MEDLINETA: J Biomed Mater Res A

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