In vitro mutagenesis of infectious viroid cDNA clone.

In vitro mutagenesis of infectious viroid cDNA clone. Research Abstract Details 

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In vitro mutagenesis of infectious viroid cDNA clone. Abstract Text:

M Ishikawa,T Meshi,Y Okada,T Sano,E Shikata,

Previously we constructed an infectious hop stunt viroid (HSV) cDNA clone, PHS-P2P, which carries two copies of full-length HSV cDNA tandemly and generates HSV RNA when it is inoculated into cucumber plants. The in vitro transcript of the cDNA clone was also infectious. To investigate the essential regions for infectivity of HSV, we introduced a short deletion or insertion into the HSV sequence of pHS-P2P using restriction sites (XhoI site for pHI-X1, PvuI site for pHI-P1, and BamHI site for pHI-B1) and assayed the infectivities of these mutagenized clones. None of these mutagenized clones and their transcripts were infectious under the conditions used. Simultaneous inoculation of two or three of non-infectious mutagenized clones or the transcripts from them did not restore the infectivity.

In vitro mutagenesis of infectious viroid cDNA clone. Publishing Authors By Initials

M Ishikawa,T Meshi,Y Okada,T Sano,E Shikata,

For similar biological phenomena, cell phenomena, and immunity: biological phenomena: microbiologic phenomena: virulence research abstracts see: biological phenomena, cell phenomena, and immunity: biological phenomena: microbiologic phenomena: virulence research

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In vitro mutagenesis of infectious viroid cDNA clone. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Journal of biochemistry

VOLUME: 98

Page Numbers: 1615-20

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: Dec

YEAR: 1985

In vitro mutagenesis of infectious viroid cDNA clone. Information

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LANGUAGE: eng

NlmUniqueID: 376600

In vitro mutagenesis of infectious viroid cDNA clone. Keywords Mesh Terms:

KEYWORDS: Virulence

MESH TERMS: pathogenicity

Chemical & Substance for Abstract: In vitro mutagenesis of infectious viroid cDNA clone. Information

Substance Name: DNA

Registry Number: 9007-49-2

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Country: JAPAN

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MEDLINETA: J Biochem

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