[In vitro manipulation of primary human osteo-progenitor cells for tissue technological bone production]

[In vitro manipulation of primary human osteo-progenitor cells for tissue technological bone production] Research Abstract Details 

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[In vitro manipulation of primary human osteo-progenitor cells for tissue technological bone production] Abstract Text:

H Schliephake,H Bertram,W Lindenmaier,M Dauner,H Mayer,

The aim of the present study was to evaluate the effect of three different techniques for isolation of human bone-marrow-derived mesenchymal stem cells (MSCs) on the in vitro differentiation of these cells. Furthermore, the use of BMP-2 was assessed and the effect of gene transfer of the BMP-2 gene into these cells was evaluated. Reverse transcription polymerase chain reaction showed that isolation of MSCs by outgrowth from cancellous chips resulted in expression of both osteogenic and chondrogenic markers, while cells cultivated from marrow aspirates exhibited only osteogenic markers. Intramuscular reimplantation of BMP-2-transfected cells in poly-(L/DL 70/30) lactic acid fleeces resulted in formation of mineralized tissue after 8 weeks in athymic rats, while control-transfected cells exhibited matrix synthesis without mineralized tissue after intramuscular reimplantation.

[In vitro manipulation of primary human osteo-progenitor cells for tissue technological bone production] Publishing Authors By Initials

H Schliephake,H Bertram,W Lindenmaier,M Dauner,H Mayer,

For similar peptides: intercellular signaling peptides and proteins: cytokines: transforming growth factor beta research abstracts see: peptides: intercellular signaling peptides and proteins: cytokines: transforming growth factor beta research

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[In vitro manipulation of primary human osteo-progenitor cells for tissue technological bone production] Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Mund-, Kiefer- und Gesichtschirurgie : MKG

VOLUME: 4 Suppl 2

Page Numbers: S470-3

Journal Abbreviation:

ISSN: 1432-9417

DAY: 19

MONTH: Sep

YEAR: 2000

[In vitro manipulation of primary human osteo-progenitor cells for tissue technological bone production] Information

Number of References:

LANGUAGE: ger

NlmUniqueID: 9716576

[In vitro manipulation of primary human osteo-progenitor cells for tissue technological bone production] Keywords Mesh Terms:

KEYWORDS: Transforming Growth Factor beta

MESH TERMS: cytology

Chemical & Substance for Abstract: [In vitro manipulation of primary human osteo-progenitor cells for tissue technological bone production] Information

Substance Name: bone morphogenetic protein 2

Registry Number: 0

Grant and Affiliation Information for [In vitro manipulation of primary human osteo-progenitor cells for tissue technological bone production]

AFFILIATION: Klinik und Poliklinik für Mund-, Kiefer- und Gesichtschirurgie, MHH Hannover.

Country: GERMANY

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ACRONYM:

MEDLINETA: Mund Kiefer Gesichtschir

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