In vitro interaction between azoles and cyclosporin A against clinical isolates of Candida albicans determined by the chequerboard method and time-kill curves.

In vitro interaction between azoles and cyclosporin A against clinical isolates of Candida albicans determined by the chequerboard method and time-kill curves. Research Abstract Details 

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In vitro interaction between azoles and cyclosporin A against clinical isolates of Candida albicans determined by the chequerboard method and time-kill curves. Abstract Text:

Yan Li,Shujuan Sun,Qiongjie Guo,Lin Ma,Changwen Shi,Lequn Su,Hongjian Li,Yan Li,Shujuan Sun,Qiongjie Guo,Lin Ma,Changwen Shi,Lequn Su,Hongjian Li,

Objectives To investigate the in vitro interaction between three azoles (fluconazole, itraconazole and voriconazole) and cyclosporin A against five azole-susceptible (azole-S) and five azole-resistant (azole-R) clinical Candida albicans isolates. Methods By using a chequerboard technique and time-kill curves, synergistic, indifferent or antagonistic effects when drugs were used in combination were assessed. In the chequerboard assay, the antifungal activity of drug combinations was determined by the microdilution method based on the CLSI M27-A2 guidelines. The effects of the interactions were assessed by two non-parametric approaches (fractional inhibitory concentration index model and DeltaE model). In the time-kill assay, a colony counting method was employed against one azole-S strain and one azole-R strain at 0, 6, 12, 24 and 48 h of incubation at 35 degrees C. Results Good concordance was found between the chequerboard method and time-kill curves. Indifference or synergism was observed for azole-S isolates in interactions of azoles and cyclosporin A, while strong synergism was observed for azole-R isolates in all drug combinations. Conclusions Cyclosporin A showed potent synergism when combined with the three azoles, especially against azole-R C. albicans strains, and there was good agreement between various methods used in this study.

In vitro interaction between azoles and cyclosporin A against clinical isolates of Candida albicans determined by the chequerboard method and time-kill curves. Publishing Authors By Initials

Y Li,S Sun,Q Guo,L Ma,C Shi,L Su,H Li,Y Li,S Sun,Q Guo,L Ma,C Shi,L Su,H Li,

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In vitro interaction between azoles and cyclosporin A against clinical isolates of Candida albicans determined by the chequerboard method and time-kill curves. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: The Journal of antimicrobial chemotherapy

VOLUME: 61

Page Numbers: 577-85

Journal Abbreviation: J. Antimicrob. Chemother.

ISSN: 1460-2091

DAY: 13

MONTH: 01

YEAR: 2008

In vitro interaction between azoles and cyclosporin A against clinical isolates of Candida albicans determined by the chequerboard method and time-kill curves. Information

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LANGUAGE: eng

NlmUniqueID: 7513617

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Grant and Affiliation Information for In vitro interaction between azoles and cyclosporin A against clinical isolates of Candida albicans determined by the chequerboard method and time-kill curves.

AFFILIATION: Department of Pharmacy, Shandong Provincial Qianfoshan Hospital, Jinan, Shandong Province, People's Republic of China.

Country: England

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MEDLINETA: J Antimicrob Chemother

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