In vitro inhibition of classical swine fever virus replication by siRNAs targeting N(pro) and NS5B genes.

In vitro inhibition of classical swine fever virus replication by siRNAs targeting N(pro) and NS5B genes. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

In vitro inhibition of classical swine fever virus replication by siRNAs targeting N(pro) and NS5B genes. Abstract Text:

Xingran Xu,Huancheng Guo,Chang Xiao,Yunfeng Zha,Zixue Shi,Xianzhu Xia,Changchun Tu,

Classical swine fever (CSF) is a highly contagious disease of pigs, which causes important economic losses worldwide. In the present study, the specific effect of RNA interference on the replication of CSF virus (CSFV) was explored. Three species of small interfering RNA (siRNA), targeting different regions of CSFV N(pro) and NS5B genes, were prepared by in vitro transcription. After transfection of PK-15 cells with each of the siRNAs followed by infection with CSFV, the viral proliferation within the cells was examined by indirect immunofluorescence microscopy. At 72h post-infection, only a few siRNA-treated cells were positive for viral antigen staining, while most untreated virus-infected cells were positive. Treatment with the siRNAs caused a 4-12-fold reduction in viral genome copy number as assessed by real time RT-PCR. Transfection with the siRNAs also suppressed the production of infectious virus by up to 467-fold as assessed by TCID(50) assay. These results suggested that the three species of siRNAs can efficiently inhibit CSFV genome replication and infectious virus production, with the inhibition persisting for 72-84h.

In vitro inhibition of classical swine fever virus replication by siRNAs targeting N(pro) and NS5B genes. Publishing Authors By Initials

X Xu,H Guo,C Xiao,Y Zha,Z Shi,X Xia,C Tu,

For similar abstracts research abstracts see: abstracts research

PUBMED ID PMID:

MEDLINE DATE:

In vitro inhibition of classical swine fever virus replication by siRNAs targeting N(pro) and NS5B genes. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Antiviral research

VOLUME: 78

Page Numbers: 188-93

Journal Abbreviation:

ISSN: 0166-3542

DAY: 24

MONTH: 01

YEAR: 2008

In vitro inhibition of classical swine fever virus replication by siRNAs targeting N(pro) and NS5B genes. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 8109699

In vitro inhibition of classical swine fever virus replication by siRNAs targeting N(pro) and NS5B genes. Keywords Mesh Terms:

KEYWORDS:

MESH TERMS:

Chemical & Substance for Abstract: In vitro inhibition of classical swine fever virus replication by siRNAs targeting N(pro) and NS5B genes. Information

Substance Name:

Registry Number:

Grant and Affiliation Information for In vitro inhibition of classical swine fever virus replication by siRNAs targeting N(pro) and NS5B genes.

AFFILIATION:

Country: Netherlands

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: Antiviral Res

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

In vitro inhibition of classical swine fever virus replication by siRNAs targeting Npro and NS5B genes Related Publications

• A systematic review of the rehabilitation of moderate to severe acquired brain injuries.
• Antioxidative responses to arsenic in the arsenic-hyperaccumulator Chinese brake fern (Pteris vittata L.).
• Decision support, knowledge representation and management in medicine.
• Estimation of survival functions in interval and right censored data using STD behavioural diaries.
• Magnesium lithospermate B possesses inhibitory activity on Na+,K+-ATPase and neuroprotective effects against ischemic stroke.
• Perlolyrine: A beta-Carboline Alkaloid from Codonopsis pilosula.
• Regional differences in process of care and outcomes for older acute myocardial infarction patients in the United States and Ontario, Canada.
• Regulation of mRNA stability through a pentobarbital-responsive element.
• Relationship between mutation of IR in the mtr system of Neisseria gonorrhoeae and multiple antibiotic resistance.
• [Analysis of the mRNA expression similarity of genes in the same gene expression regulatory pathway]
• [Attention should be paid to prevention and control of complications of thoracic sympathicotomy for palmar hyperhidrosis]
• [Changes of Clara cell protein and surfactant protein-D in serum of patients with silicosis]
• [Construction of promoter-trap library screening in vivo-induced gene in Streptococcus pneumoniae]
• [Determination of V, Mo, Ni, g in Chinese traditional cardiovascular medicine by AAS]
• [Determination of multiple pesticide residues in honey using gas chromatography-mass spectrometry]
• [Effect of microgravity simulation with clinostat on different strains in Anabeana oryza]
• [Establishing DOTS-Plus strategy for control of multi-drug resistant tuberculosis]
• [Evaluation of eustachian tube function with impedance audiometry in chronic suppurative otitis media]
• [Evidence-based medicine on the relationship between Helicobacter pylori and gastroesophageal reflux disease]
• [Factors influencing the use of health care services from patients with COPD in Chengdu]
• [Gas-liquid mass transfer in three-phase inverse turbulent bed reactor]
• [Influence of high- and low-osmolality contrast media on renal function in children]
• [Macrophage activation syndrome: genetic basis and therapeutic hypothesis]
• [Molecular genetic diversity of Fujian domestic duck breeds]
• [No arytenoidectomy for early lesions of glottic cancer]
• [Omega-mercaptohexanoic acid self-assemble-modified gold electrode and its catalytic property for redox reaction of chlorophylls]
• [Simultaneous determination of twelve glucocorticoids residues in milk by ultra performance liquid chromatography-electrospray tandem mass spectrometry]
• [Studies on chemical constituents from root of Clematis hexapetala]
• [The clinical significance of inspiratory capacity measurement in patients with stable chronic obstructive pulmonary disease]
• [Utilizing 2-DE and MALDI-TOF MS/MS to screen differentially expressed serum proteins of silicosis]