In vitro hypoxic preconditioning of embryonic stem cells as a strategy of promoting cell survival and functional benefits after transplantation into the ischemic rat brain.

In vitro hypoxic preconditioning of embryonic stem cells as a strategy of promoting cell survival and functional benefits after transplantation into the ischemic rat brain. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

In vitro hypoxic preconditioning of embryonic stem cells as a strategy of promoting cell survival and functional benefits after transplantation into the ischemic rat brain. Abstract Text:

Michelle Hedrick Theus,Ling Wei,Lin Cui,Kevin Francis,Xinyang Hu,Christine Keogh,Shan Ping Yu,

Hypoxic preconditioning (HP) and stem cell transplantation have been extensively studied as individual therapies for ischemic stroke. The present investigation is an initial effort to combine these methods to achieve increased therapeutic effects after brain ischemia. Sublethal in vitro hypoxia pretreatment significantly enhanced the tolerance of neurally-differentiating embryonic stem (ES) cells and primary bone marrow mesenchymal stem cells (BMSC) to apoptotic cell death (40-50% reduction in cell death and caspase-3 activation). The HP protective effects on cultured cells lasted for at least 6 days. HP increased secretion of erythropoietin (EPO) and upregulated expression of bcl-2, hypoxia-inducible factor (HIF-1alpha), erythropoietin receptor (EPOR), neurofilament (NF), and synaptophysin in ES cell-derived neural progenitor cells (ES-NPCs). The HP cytoprotective effect was diminished by blocking EPOR, while pretreatment of ES-NPCs with recombinant human EPO mimicked the HP effect. HP-primed ES-NPCs survived better 3 days after transplantation into the ischemic brain (30-40% reduction in cell death and caspase-3 activation). Finally, transplanted HP-primed ES-NPCs exhibited extensive neuronal differentiation in the ischemic brain, accelerated and enhanced recovery of sensorimotor function when compared to transplantation of non-HP-treated ES-NPCs. The cell-priming strategy aimed to promote transplanted cell survival and their tissue repair capability provides a simple yet effective way of optimizing cell transplantation therapy.

In vitro hypoxic preconditioning of embryonic stem cells as a strategy of promoting cell survival and functional benefits after transplantation into the ischemic rat brain. Publishing Authors By Initials

MH Theus,L Wei,L Cui,K Francis,X Hu,C Keogh,SP Yu,

For similar abstracts research abstracts see: abstracts research

PUBMED ID PMID:

MEDLINE DATE:

In vitro hypoxic preconditioning of embryonic stem cells as a strategy of promoting cell survival and functional benefits after transplantation into the ischemic rat brain. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Experimental neurology

VOLUME: 210

Page Numbers: 656-70

Journal Abbreviation: Exp. Neurol.

ISSN: 0014-4886

DAY: 5

MONTH: 01

YEAR: 2008

In vitro hypoxic preconditioning of embryonic stem cells as a strategy of promoting cell survival and functional benefits after transplantation into the ischemic rat brain. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 370712

In vitro hypoxic preconditioning of embryonic stem cells as a strategy of promoting cell survival and functional benefits after transplantation into the ischemic rat brain. Keywords Mesh Terms:

KEYWORDS:

MESH TERMS:

Chemical & Substance for Abstract: In vitro hypoxic preconditioning of embryonic stem cells as a strategy of promoting cell survival and functional benefits after transplantation into the ischemic rat brain. Information

Substance Name:

Registry Number:

Grant and Affiliation Information for In vitro hypoxic preconditioning of embryonic stem cells as a strategy of promoting cell survival and functional benefits after transplantation into the ischemic rat brain.

AFFILIATION: Department of Pathology and Laboratory Medicine, Medical University of South Carolina, Charleston, SC 29425, USA; Department of Pharmaceutical and Biomedical Sciences, Medical University of South Carolina, Charleston, SC 29425, USA.

Country: United States

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: Exp Neurol

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

In vitro hypoxic preconditioning of embryonic stem cells as a strategy of promoting cell survival and functional benefits after transplantation into the ischemic rat brain Related Publications

• An overview of vagal airway receptors.
• Element mixing distribution and structure feature of fusion zone in laser welding between different alloys and pure titanium.
• Endothelial function and carotid artery wall thickening in patients with early essential hypertension.
• Genetic factors underlying prostate cancer.
• Genotype-phenotype analysis for the polymorphic CA repeat in the insulin-like growth factor-I (IGF-I) gene.
• Na(+)/K(+) -ATPase as a marker for detecting pulmonary sensory receptors.
• RRR-alpha-tocopheryl succinate inhibits human gastric cancer SGC-7901 cell growth by inducing apoptosis and DNA synthesis arrest.
• The effects of vitamin E succinate on the expression of c-jun gene and protein in human gastric cancer SGC-7901 cells.
• [A case of sepsis and multiple organ dysfunction syndrome complicated by Staphylococcus infection]
• [Anisodamine in prevention and treatment of sepsis of severely burned patients]
• [Association study on HLA-DP and -DQ allelic polymorphisms and nasopharyngeal carcinoma in the Han nationality in Hunan province]
• [Change of catecholamine in serum and hippocampus of rats after electromagnetic irradiation and the selection of protective site]
• [Changes of phosphorylation states of Cx43 protein in Chinese hamster lung cells induced by SiO2]
• [Double uterus and hydatidiform mole in the malformed uterus: report of one case]
• [Early diagnosis of pancreatic cancer is essential to improving the effect of treatment]
• [Effect of oxidated low-density lipoprotein on early apoptosis of vascular smooth muscle cell]
• [Extinguishment of harmful algae by organo-clay]
• [Humoral immune response in mice to hybrid nucleic acid vaccines containing Plasmodium falciparum merozoite surface protein 1 block 17-based gene]
• [PCR in detecting the correlation between infection of HBV and cholelithiasis]
• [Post-mastectomy radiotherapy with different fractionated dose schemes in early breast cancer]
• [Primary extranodal lymphomas: analyses of clinical characteristics and misdiagnoses of 139 cases]
• [Relationship between Helicobacter pylori infection and expression of c-myc, Bcl-2, and Bax protein in different gastric mucosa lesions]
• [Researches in forensic biomechanics]
• [Study on antitumor effect and mechanism of aloe polysaccharides]
• [Study on the genic polymorphism of HLA-DRB1 in a population in the north of China]
• [The effect of radix Pseudostellariae from 8 habitats on spleen-deficiency and immunologic function]
• [The expression and activity of caspase-8 in the process of vitamin E succinate-induced apoptosis in human gastric carcinoma SGC-7901 cells]
• [Treatment of burns combined injury]
• [Wavelet feature extraction and classification of Doppler ultrasound blood flow signals]
• [p15 gene expression in acute lymphoblastic leukemia cell line Molt4 induced by arsenic trioxide]