In vitro glucuronidation of estradiol-17beta by microsomes prepared using liver biopsy specimens from dairy cows.

In vitro glucuronidation of estradiol-17beta by microsomes prepared using liver biopsy specimens from dairy cows. Research Abstract Details 

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In vitro glucuronidation of estradiol-17beta by microsomes prepared using liver biopsy specimens from dairy cows. Abstract Text:

Hiroya Kadokawa,Junya Matsumoto,Hiroshi Yokota,Hiroya Kadokawa,Junya Matsumoto,Hiroshi Yokota,

Increased hepatic metabolism of estradiol may cause weakened estrous behavior in lactating dairy cows, but this hypothesis must be examined further, especially through diachronic study of the hepatic estradiol-17beta glucuronidation activity of uridine diphosphate (UDP)-glucuronosyltransferases. Therefore, in order to develop a new tool for this purpose, we attempted to conduct biopsy of the livers of dairy cows with the aid of ultrasonography and to measure the UDP-glucuronosyltransferase activities of microsomes of the specimens using in vitro glucuronidation followed by HPLC analysis. We were able to measure the activities of the microsomes prepared from the liver biopsy, and the results seemed reliable. Therefore, this method may become a new tool in clinical studies to detect estradiol-17beta glucuronidation activity.

In vitro glucuronidation of estradiol-17beta by microsomes prepared using liver biopsy specimens from dairy cows. Publishing Authors By Initials

H Kadokawa,J Matsumoto,H Yokota,H Kadokawa,J Matsumoto,H Yokota,

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In vitro glucuronidation of estradiol-17beta by microsomes prepared using liver biopsy specimens from dairy cows. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: The Journal of veterinary medical science / the Ja

VOLUME: 69

Page Numbers: 557-9

Journal Abbreviation: J. Vet. Med. Sci.

ISSN: 0916-7250

DAY: 6

MONTH: May

YEAR: 2007

In vitro glucuronidation of estradiol-17beta by microsomes prepared using liver biopsy specimens from dairy cows. Information

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LANGUAGE: eng

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Grant and Affiliation Information for In vitro glucuronidation of estradiol-17beta by microsomes prepared using liver biopsy specimens from dairy cows.

AFFILIATION: Department of Veterinary Medicine, Faculty of Agriculture, Yamaguchi University, Yoshida, Yamaguchi, Japan.

Country: Japan

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MEDLINETA: J Vet Med Sci

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