In vitro expansion of tissue cells by conditional proliferation.

In vitro expansion of tissue cells by conditional proliferation. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

In vitro expansion of tissue cells by conditional proliferation. Abstract Text:

Tobias May, Hauser,Dagmar Wirth,Tobias May, Hauser,Dagmar Wirth,

Cell therapies rely on the implantation of well-characterized functional cells with defined properties. Often, the cells of interest do not proliferate in vitro and thus cannot be expanded to the amount needed for characterization, purification, manipulation, or cloning. Here, we describe a method that allows the reversible expansion of cells by the introduction of a proliferator gene controlled by a regulatable expression module. The module is transferred by DNA transfer or by lentiviral transduction. The addition of a clinically accepted regulator [Doxycycline (Dox)] induces proliferator expression and expansion of the cells ad infinitum. Removal of the regulator eliminates the effect of the proliferator and leaves the cells in a non-proliferating status. The method has been applied to different mouse and human tissues. This chapter describes the method for the well-examined example of mouse embryonic fibroblast (MEF) expansion.

In vitro expansion of tissue cells by conditional proliferation. Publishing Authors By Initials

T May,H Hauser,D Wirth,T May,H Hauser,D Wirth,

For similar abstracts research abstracts see: abstracts research

PUBMED ID PMID:

MEDLINE DATE:

In vitro expansion of tissue cells by conditional proliferation. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Methods in molecular medicine

VOLUME: 140

Page Numbers: 1-15

Journal Abbreviation: Methods Mol. Med.

ISSN: 1543-1894

DAY: 18

MONTH: 12

YEAR: 2007

In vitro expansion of tissue cells by conditional proliferation. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 101123138

In vitro expansion of tissue cells by conditional proliferation. Keywords Mesh Terms:

KEYWORDS:

MESH TERMS:

Chemical & Substance for Abstract: In vitro expansion of tissue cells by conditional proliferation. Information

Substance Name:

Registry Number:

Grant and Affiliation Information for In vitro expansion of tissue cells by conditional proliferation.

AFFILIATION: Department of Gene Regulation and Differentiation, National Research Center for Biotechnology GBF, Braunschweig, Germany.

Country: United States

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: Methods Mol Med

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

In vitro expansion of tissue cells by conditional proliferation Related Publications

• BOB.1/OBF.1 controls the balance of TH1 and TH2 immune responses.
• Defining the origin of Plasmodium falciparum resistant dhfr isolates in Senegal.
• Effect of atrial electrical remodeling on the efficacy of antiarrhythmic drugs: comparison of amiodarone with I(Kr)- and I(to)/IKur-blockade in vivo.
• Electromigration of single molecules of DNA in a crystalline array of 300-nm silica colloids.
• High-speed electroseparations inside silica colloidal crystals.
• In vitro expansion of tissue cells by conditional proliferation.
• Incorporating patient self-testing into your practice.
• Interferometric measurements of the surface profile of moving samples.
• Lectin binding studies on C-28/I2 and T/C-28a2 chondrocytes provide a basis for new tissue engineering and drug delivery perspectives in cartilage research.
• Magnetic resonance imaging assessment of spinal inflammation in patients treated for ankylosing spondylitis.
• Mass transport in sub-2-microm high-performance liquid chromatography.
• Materials science. How does radiation damage materials?
• Non-specific association between filamentous bacteria and fungus-growing ants.
• Parsing the motion of single molecules: a novel algorithm for deconvoluting the dynamics of individual receptors at the cell surface.
• Physiological and serological variation in Streptococcus mitis biovar 1 from the human oral cavity during the first year of life.
• Scopolamine in vertigo.
• Separation media for microchips.
• Silica colloidal crystals for enhanced fluorescence detection in microarrays.
• Sintered silica colloidal crystals with fully hydroxylated surfaces.
• The kinin antagonist hoe 140 reduces acute paw oedema in rats caused by carrageenan, bradykinin and kaolin.
• The rationale and role of radiation therapy in the treatment of patients with diffuse large B-cell lymphoma in the Rituximab era.
• The role of DNA mismatch repair in generating genetic diversity and drug resistance in malaria parasites.
• Twenty-year results of combined meniscal allograft transplantation, anterior cruciate ligament reconstruction and advancement of the medial collateral ligament.
• [In Process Citation]
• [In Process Citation]
• [Not Available.]
• [Not Available.]
• [Not Available.]
• [Pharmacology of hibernation.]
• [The influence of nCPAP therapy on the autonomic nervous system dysfunction in OSA patients]