In vitro development of mouse fetal germ cells into mature oocytes.

In vitro development of mouse fetal germ cells into mature oocytes. Research Abstract Details 

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In vitro development of mouse fetal germ cells into mature oocytes. Abstract Text:

Wei Shen,Lan Li,Zhaodai Bai,Qingjie Pan,Mingxiao Ding,Hongkui Deng,

Little is known about the mechanisms underlying primordial follicular formation and the acquisition of competence to resume meiosis by growing oocytes. It is therefore important to establish an in vitro experimental model that allows one to study such mechanisms. Mouse follicular development has been studied in vitro over the past several years; however, no evidence has been presented showing that mature oocytes can be obtained from mouse fetal germ cells prior to the formation of primordial follicles. In this study, a method has been established to obtain mature oocytes from the mouse fetal germ cells at 16.5 days postcoitum (dpc). From the initiation of primordial follicular formation to the growth of early secondary follicles, ovarian tissues from 16.5 dpc fetal mice were cultured in vitro for 14 days. Subsequently, 678 intact secondary follicles were isolated from 182 mouse fetal ovaries and cultured for 12 days. A total of 141 oocytes inside antral follicles were matured in vitro, and 102 oocytes underwent germinal vesicle breakdown. We found that 97 oocytes were fertilized and 15 embryos were able to form morula-blastocysts. We also analyzed various genomic imprinting markers and showed that the erasure of genomic imprinting markers in the parental generation was also imposed on the oocytes that developed from fetal germ cells. Our results demonstrate that mouse fetal germ cells are able to form primordial follicles with ovarian cells, and that oocytes within the growing follicles are able to mature normally in vitro.

In vitro development of mouse fetal germ cells into mature oocytes. Publishing Authors By Initials

W Shen,L Li,Z Bai,Q Pan,M Ding,H Deng,

For similar investigative techniques: clinical laboratory techniques: culture techniques: organ culture techniques research abstracts see: investigative techniques: clinical laboratory techniques: culture techniques: organ culture techniques research

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In vitro development of mouse fetal germ cells into mature oocytes. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Reproduction (Cambridge, England)

VOLUME: 134

Page Numbers: 223-31

Journal Abbreviation: Reproduction

ISSN: 1470-1626

DAY: 27

MONTH: Aug

YEAR: 2007

In vitro development of mouse fetal germ cells into mature oocytes. Information

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LANGUAGE: eng

NlmUniqueID: 100966036

In vitro development of mouse fetal germ cells into mature oocytes. Keywords Mesh Terms:

KEYWORDS: Organ Culture Techniques

MESH TERMS: cytology

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Grant and Affiliation Information for In vitro development of mouse fetal germ cells into mature oocytes.

AFFILIATION: College of Animal Science and Technology, Institute of Animal Reproduction Development and Genetic Engineering, Qingdao Agricultural University, Qingdao 266109, China. shenwei427@yahoo.com.cn

Country: England

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MEDLINETA: Reproduction

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