In vitro and in vivo synergistic interactions between the Runx2/Cbfa1 transcription factor and bone morphogenetic protein-2 in stimulating osteoblast differentiation.

In vitro and in vivo synergistic interactions between the Runx2/Cbfa1 transcription factor and bone morphogenetic protein-2 in stimulating osteoblast differentiation. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

In vitro and in vivo synergistic interactions between the Runx2/Cbfa1 transcription factor and bone morphogenetic protein-2 in stimulating osteoblast differentiation. Abstract Text:

Shuying Yang,Daoyan Wei,Dian Wang,Mattabhorn Phimphilai,Paul H Krebsbach,Renny T Franceschi,

Bone regeneration requires interactions between a number of factors including bone morphogenetic proteins (BMPs), growth factors, and transcriptional regulators such as Runx2/Cbfal (Runx2). Because each component may provide a unique contribution to the overall osteogenic response, we hypothesized that bone formation may be enhanced by using combinations of complimentary factors. As an initial test of this concept, interactions between BMP2 and Runx2 were examined using adenovirus-based expression vectors (AdCMV-Runx2, AdCMV-BMP2) in the pluripotent C3H10T1/2 cell line. Cells transduced with AdCMV-Runx2 strongly expressed osteoblast markers, such as alkaline phosphatase and osteocalcin, but formed only a weakly mineralized extracellular matrix in vitro, whereas cells transduced with AdCMV-BMP2 exhibited higher levels of mineralization, but only expressed low levels of Runx2 and osteocalcin mRNA. Significantly, when cells were transduced with optimal titers of both viruses, osteoblast differentiation was stimulated to levels that were 10-fold greater than those seen with either AdCMV-Runx2 or AdCMV-BMP2 alone. To measure in vivo osteogenic activity, virally transduced cells were subcutaneously implanted into immunodeficient mice. Cells transduced with control virus produced only fibrous tissue while those with AdCMV-Runx2 produced limited amounts of both cartilage and bone. In contrast, cells transduced with either AdCMV-BMP2 alone or AdCMV-BMP2 plus AdCMV-Cbfal generated large ossicles containing cartilage, bone, and a marrow cavity. However, ossification in the AdCMV-BMP2 plus AdCMV-Cbfal group was more extensive in that both mineral content and fractional bone area were greater than that seen in the AdCMV-BMP2 group. Thus, the increased osteoblast differentiation observed with combined adenovirus treatment in vitro is also manifested by increased bone formation in vivo. These results suggest that Runx2 and BMP2 have distinct, but complementary, roles in osteogenesis and that their combined actions may be necessary for optimal bone formation.

In vitro and in vivo synergistic interactions between the Runx2/Cbfa1 transcription factor and bone morphogenetic protein-2 in stimulating osteoblast differentiation. Publishing Authors By Initials

S Yang,D Wei,D Wang,M Phimphilai,PH Krebsbach,RT Franceschi,

For similar peptides: intercellular signaling peptides and proteins: cytokines: transforming growth factor beta research abstracts see: peptides: intercellular signaling peptides and proteins: cytokines: transforming growth factor beta research

PUBMED ID PMID:

MEDLINE DATE:

In vitro and in vivo synergistic interactions between the Runx2/Cbfa1 transcription factor and bone morphogenetic protein-2 in stimulating osteoblast differentiation. Journal Published:

PUBLICATION TYPE: Research Support, U.S. Gov't,

Journal: Journal of bone and mineral research : the officia

VOLUME: 18

Page Numbers: 705-15

Journal Abbreviation: J. Bone Miner. Res.

ISSN: 0884-0431

DAY: 19

MONTH: Apr

YEAR: 2003

In vitro and in vivo synergistic interactions between the Runx2/Cbfa1 transcription factor and bone morphogenetic protein-2 in stimulating osteoblast differentiation. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 8610640

In vitro and in vivo synergistic interactions between the Runx2/Cbfa1 transcription factor and bone morphogenetic protein-2 in stimulating osteoblast differentiation. Keywords Mesh Terms:

KEYWORDS: Transforming Growth Factor beta

MESH TERMS: metabolism

Chemical & Substance for Abstract: In vitro and in vivo synergistic interactions between the Runx2/Cbfa1 transcription factor and bone morphogenetic protein-2 in stimulating osteoblast differentiation. Information

Substance Name: bone morphogenetic protein 2

Registry Number: 0

Grant and Affiliation Information for In vitro and in vivo synergistic interactions between the Runx2/Cbfa1 transcription factor and bone morphogenetic protein-2 in stimulating osteoblast differentiation.

AFFILIATION: Department of Periodontics, Prevention, and Geriatrics, School of Dentistry, University of Michigan, Ann Arbor, Michigan 48109-1078, USA.

Country: United States

AGENCY: United States NIDCR

GRANT: DE13835

ACRONYM: DE

MEDLINETA: J Bone Miner Res

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

In vitro and in vivo synergistic interactions between the Runx2/Cbfa1 transcription factor and bone morphogenetic protein-2 in stimulating osteoblast differentiation Related Publications

• A genetic-demographic approach reveals male-specific association between survival and tumor necrosis factor (a/g)-308 polymorphism.
• A novel mitochondrial DNA-like sequence insertion polymorphism in Intron I of the FOXO1A gene.
• Analysis of transcription factor interactions in osteoblasts using competitive chromatin immunoprecipitation.
• Back to the future: the ribosome as an antibiotic target.
• Bone morphogenetic proteins, extracellular matrix, and mitogen-activated protein kinase signaling pathways are required for osteoblast-specific gene expression and differentiation in MC3T3-E1 cells.
• Chapter 27: Research needs following initial licensure of virus-like particle HPV vaccines.
• Common evolutionary origin of the immune and neuroendocrine systems: from morphological and functional evidence to in silico approaches.
• Critical role of the extracellular signal-regulated kinase-MAPK pathway in osteoblast differentiation and skeletal development.
• Daunorubicin and daunorubicinol tissue concentrations in gastric cancer patients after local administration of a liposomal preparation.
• Different types of cell death in organismal aging and longevity: state of the art and possible systems biology approach.
• Erythrocyte plasma membranes obtained from centenarians show different functional properties.
• Healing Cranial Defects with AdRunx2-transduced Marrow Stromal Cells.
• Human longevity within an evolutionary perspective: The peculiar paradigm of a post-reproductive genetics.
• Human papillomavirus infection in women with and without cervical cancer in Warsaw, Poland.
• Human papillomavirus types among women infected with HIV: a meta-analysis.
• Immunoproteasome in Macaca fascicularis: No Age-Dependent Modification of Abundance and Activity in the Brain and Insight into an in silico Structural Model.
• In vitro and in vivo synergistic interactions between the Runx2/Cbfa1 transcription factor and bone morphogenetic protein-2 in stimulating osteoblast differentiation.
• Incidence of primary liver cancer in Italy between 1988 and 2002: An age-period-cohort analysis.
• Inflammaging as a major characteristic of old people: can it be prevented or cured?
• Long-term immune-endocrine effects of bereavement: relationships with anxiety levels and mood.
• Members of the human papillomavirus type 18 family (alpha-7 species) share a common association with adenocarcinoma of the cervix.
• New molecular targets and novel anticancer treatments: emerging trends in neuro-oncology.
• Paraoxonase 1: genetics and activities during aging.
• Plasma concentrations of interleukin-1-beta, interleukin-6 and tumor necrosis factor-alpha, and of their soluble receptors and receptor antagonist in anorexia nervosa.
• Prevalence of hepatitis B and C serological markers among first-time blood donors in Brazil: a multi-center serosurvey.
• Risk of cancer following immunosuppression in organ transplant recipients and in HIV-positive individuals in southern Europe.
• Serologic response to oncogenic human papillomavirus types in male and female university students in Busan, South Korea.
• The IARC commitment to cancer prevention: the example of papillomavirus and cervical cancer.
• Use of a stringent dimerizer-regulated gene expression system for controlled BMP2 delivery.
• Usefulness of the comprehensive geriatric assessment in older patients with upper gastrointestinal bleeding: a two-year follow-up study.