In vitro and in vivo osteoblastic differentiation of BMP-2- and Runx2-engineered skeletal myoblasts.

In vitro and in vivo osteoblastic differentiation of BMP-2- and Runx2-engineered skeletal myoblasts. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

In vitro and in vivo osteoblastic differentiation of BMP-2- and Runx2-engineered skeletal myoblasts. Abstract Text:

Charles A Gersbach,Robert E Guldberg, ,

Genetic engineering with osteogenic factors is a promising approach for cell-based therapeutics and orthopedic regeneration. However, the relative efficacy of different strategies for inducing osteoblastic differentiation remains unclear and is further complicated by varied delivery vehicles, cell types, and evaluation criteria. In order to elucidate the effects of distinct gene-based strategies, we quantitatively evaluated osteoblastic differentiation and mineralization of primary skeletal myoblasts overexpressing either the BMP-2 growth factor or Runx2 transcription factor. Retroviral delivery of BMP-2 or Runx2 stimulated differentiation into an osteoblastic phenotype, as demonstrated by the induction of osteogenic gene expression, alkaline phosphatase activity, and matrix mineralization in monolayer culture and on collagen scaffolds both in vitro and in an intramuscular site in vivo. In general, BMP-2 stimulated osteoblastic markers faster and to a greater extent than Runx2, although we also identified experimental conditions under which these two factors produced similar effects. Additionally, Runx2-engineered cells did not utilize paracrine signaling via secreted osteogenic factors, in contrast to cells overexpressing BMP-2, as demonstrated by conditioned media studies and activation of Smad signaling. These results emphasize the complexity of gene therapy-based orthopedic therapeutics as an integrated relationship of differentiation state, construct maturation, and paracrine signaling of osteogenic cells. This study is significant in evaluating proposed therapeutic systems and defining a successful strategy for integrating gene medicine and orthopedic regeneration.

In vitro and in vivo osteoblastic differentiation of BMP-2- and Runx2-engineered skeletal myoblasts. Publishing Authors By Initials

CA Gersbach,RE Guldberg,AJ ,

For similar peptides: intercellular signaling peptides and proteins: cytokines: transforming growth factor beta: transforming growth factor beta1 research abstracts see: peptides: intercellular signaling peptides and proteins: cytokines: transforming growth factor beta: transforming growth factor beta1 research

PUBMED ID PMID:

MEDLINE DATE:

In vitro and in vivo osteoblastic differentiation of BMP-2- and Runx2-engineered skeletal myoblasts. Journal Published:

PUBLICATION TYPE: Research Support, U.S. Gov't,

Journal: Journal of cellular biochemistry

VOLUME: 100

Page Numbers: 1324-36

Journal Abbreviation: J. Cell. Biochem.

ISSN: 0730-2312

DAY: 1

MONTH: Apr

YEAR: 2007

In vitro and in vivo osteoblastic differentiation of BMP-2- and Runx2-engineered skeletal myoblasts. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 8205768

In vitro and in vivo osteoblastic differentiation of BMP-2- and Runx2-engineered skeletal myoblasts. Keywords Mesh Terms:

KEYWORDS: Transforming Growth Factor beta1

MESH TERMS: metabolism

Chemical & Substance for Abstract: In vitro and in vivo osteoblastic differentiation of BMP-2- and Runx2-engineered skeletal myoblasts. Information

Substance Name: bone morphogenetic protein 2

Registry Number: 0

Grant and Affiliation Information for In vitro and in vivo osteoblastic differentiation of BMP-2- and Runx2-engineered skeletal myoblasts.

AFFILIATION: Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, Atlanta, Georgia 30332, USA.

Country: United States

AGENCY: United States NIGMS

GRANT: T32-GM08433

ACRONYM: GM

MEDLINETA: J Cell Biochem

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

In vitro and in vivo osteoblastic differentiation of BMP-2- and Runx2-engineered skeletal myoblasts Related Publications

• 1alpha-Angeloyloxy-8beta,10beta-dihydroxyeremophil-7(ll)-8alpha,12-olide from Roldana sessilifolia1.
• 99mTc-MAG3 renography: normal values for MAG3 clearance and curve parameters, excretory parameters, and residual urine volume.
• Attenuation correction reveals gender-related differences in the normal values of transient ischemic dilation index in rest-exercise stress sestamibi myocardial perfusion imaging.
• Behavioral genetics of caenorhabditis elegans unc-103-encoded erg-like K(+) channel.
• Brain stimulation for epilepsy: stimulating results?
• Constrictive pericarditis after coronary artery bypass.
• Critical care issues in the early management of severe trauma.
• Exon-based mapping of microarray probes: recovering differential gene expression signal in underpowered hypoxia experiment.
• Familial spontaneous pneumothorax.
• Frequency modulation in the transmittivity of wave guides in elastic-wave band-gap materials.
• Genomics of acute lung injury.
• Gingivitis susceptibility and its relation to periodontitis in men.
• Humanized mice mount specific adaptive and innate immune responses to EBV and TSST-1.
• In vitro and in vivo osteoblastic differentiation of BMP-2- and Runx2-engineered skeletal myoblasts.
• Lentivirus Nef specifically activates Pak2.
• Medical intractability and imaging: can MRI predict the future?
• Microarray analysis of regional cellular responses to local mechanical stress in acute lung injury.
• Milk progesterone profiles in crossbred Brown Swiss x Nellore cattle following natural service.
• Mineralization capacity of Runx2/Cbfa1-genetically engineered fibroblasts is scaffold dependent.
• Mood disorders and epilepsy surgery: lightening the burden in more ways than one?
• Network architecture for global biomedical monitoring service.
• Preliminary evaluation of adherence on abiotic and cellular surfaces of Acinetobacter baumannii strains isolated from catheter tips.
• Risk of tooth loss after cigarette smoking cessation.
• Secologanin and Derivatives from Gentiana verna.
• Structural and mechanistic insights into nerve growth factor interactions with the TrkA and p75 receptors.
• The impact of literacy on health-related quality of life measurement and outcomes in cancer outpatients.
• Tobacco use and incidence of tooth loss among US male health professionals.
• Voltammetric determination of vitamin D(3) with a rotating glassy carbon electrode.
• Whither neurosurgery?
• [Collective imaginary: lyricism in public health]