In vitro amplification and detection of variant Creutzfeldt-Jakob disease PrPSc.

In vitro amplification and detection of variant Creutzfeldt-Jakob disease PrPSc. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

In vitro amplification and detection of variant Creutzfeldt-Jakob disease PrPSc. Abstract Text:

M Jones,A H Peden,C V Prowse,A ,J C Manson,M L Turner,J W Ironside,I R MacGregor,M W Head,M Jones,A H Peden,C V Prowse,A ,J C Manson,M L Turner,J W Ironside,I R MacGregor,M W Head,

Variant Creutzfeldt-Jakob disease (vCJD) poses a serious risk of secondary transmission and the need to detect infectivity in asymptomatic individuals is therefore of major importance. Following infection, it is assumed that minute amounts of disease-associated prion protein (PrP(Sc)) replicate by conversion of the host cellular prion protein (PrP(C)). Therefore, methods of rapidly reproducing this conversion process in vitro would be valuable tools in the development of such tests. We show that one such technique, protein misfolding cyclic amplification (PMCA), can amplify vCJD PrP(Sc) from human brain tissue, and that the degree of amplification is dependent upon the substrate PRNP codon 129 polymorphism. Both human platelets and transgenic mouse brain are shown to be suitable alternative substrate sources, and amplified PrP(Sc) can be detected using a conformation-dependent immunoassay (CDI), allowing the detection of putative proteinase K sensitive forms of PrP(Sc).

In vitro amplification and detection of variant Creutzfeldt-Jakob disease PrPSc. Publishing Authors By Initials

M Jones,AH Peden,CV Prowse,A ,JC Manson,ML Turner,JW Ironside,IR MacGregor,MW Head,M Jones,AH Peden,CV Prowse,A ,JC Manson,ML Turner,JW Ironside,IR MacGregor,MW Head,

For similar abstracts research abstracts see: abstracts research

PUBMED ID PMID:

MEDLINE DATE:

In vitro amplification and detection of variant Creutzfeldt-Jakob disease PrPSc. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: The Journal of pathology

VOLUME: 213

Page Numbers: 21-6

Journal Abbreviation: J. Pathol.

ISSN: 0022-3417

DAY: 16

MONTH: Sep

YEAR: 2007

In vitro amplification and detection of variant Creutzfeldt-Jakob disease PrPSc. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 204634

In vitro amplification and detection of variant Creutzfeldt-Jakob disease PrPSc. Keywords Mesh Terms:

KEYWORDS:

MESH TERMS:

Chemical & Substance for Abstract: In vitro amplification and detection of variant Creutzfeldt-Jakob disease PrPSc. Information

Substance Name:

Registry Number:

Grant and Affiliation Information for In vitro amplification and detection of variant Creutzfeldt-Jakob disease PrPSc.

AFFILIATION: National CJD Surveillance Unit, School of Molecular and Clinical Medicine (Pathology), University of Edinburgh, Western General Hospital, Edinburgh EH4 2XU, UK. mjones@staffmail.ed.ac.uk

Country: England

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: J Pathol

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

In vitro amplification and detection of variant Creutzfeldt-Jakob disease PrPSc Related Publications

• A multi-state model for joint modelling of terminal and non-terminal events with application to Whitehall II.
• Actinomycetoma caused by Nocardiopsis dassonvillei.
• Actinomycetoma caused by Nocardiopsis dassonvillei.
• Alpha- and beta-secretase activity as a function of age and beta-amyloid in Down syndrome and normal brain.
• Canadian contributions to health in the developing world.
• Combining an antioxidant-fortified diet with behavioral enrichment leads to cognitive improvement and reduced brain pathology in aging canines: strategies for healthy aging.
• Critical role of Arg/Lys343 in the species-dependent recognition of phosphatidylinositol by pulmonary surfactant protein D.
• Effects on blood pressure do not explain the association between organizational justice and coronary heart disease in the Whitehall II study.
• Elastosis perforans serpiginosa and other complications.
• Expression profiling of glycosyltransferases and related enzymes using gene microarrays.
• How and why nurses should contribute to submissions.
• In vitro amplification and detection of variant Creutzfeldt-Jakob disease PrPSc.
• Infected intrapulmonary bronchial cyst.
• Is treatment needed?
• Lack of pathology in a triple transgenic mouse model of Alzheimer's disease after overexpression of the anti-apoptotic protein bcl-2.
• Levels of renal and extrarenal sympathetic drive in angiotensin II-induced hypertension.
• Low medically certified sickness absence among employees with poor health status predicts future health improvement: the Whitehall II study.
• Maternal and paternal condition effects on offspring phenotype in Telostylinus angusticollis (Diptera: Neriidae).
• Neuroprotective effects of cognitive enrichment.
• Partnering occupational therapy and community development.
• Progress in the treatment of emphysema.
• Radial head-capitellum view in elbow trauma.
• Salivary duct carcinoma: a clinical and histologic review with implications for trastuzumab therapy.
• Seven-year follow-up on 334 patients treated by breast conserving surgery and short course radical postoperative radiotherapy: a report of the Yorkshire Breast Cancer Group.
• Supplementing holstein cows with low doses of bovine somatotropin prepartum and postpartum reduces calving-related diseases.
• The biogeographical distribution of closely related freshwater sediment bacteria is determined by environmental selection.
• The use of interstitial radon seeds and needles in inoperable lung cancer.
• Transcriptional profiling and pathway analysis of monosodium iodoacetate-induced experimental osteoarthritis in rats: relevance to human disease.
• Transthoracic nephrectomy (right) for tuberculosis of the kidney.
• Upregulation of the vascular alpha-1 receptor in malignant DOCA-salt hypertension.