In situ observation of bovine serum albumin-adsorbed stearic acid monolayer by Brewster angle microscopy.

In situ observation of bovine serum albumin-adsorbed stearic acid monolayer by Brewster angle microscopy. Research Abstract Details 

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In situ observation of bovine serum albumin-adsorbed stearic acid monolayer by Brewster angle microscopy. Abstract Text:

H Tanaka,T Akatsuka,T Murakami,Y Ogoma,K Abe,Y Kondo,

The morphology of protein-adsorbed stearic acid monolayers containing a fluorescent probe (rhodamine B octadecylester perchloride: RBO) was observed by using fluorescence spectroscopy, fluorescence microscopy, and Brewster angle microscopy (BAM). The quenching of fluorescence of RBO was observed at the limiting area of the stearic acid monolayer. Thus, the fluorescence of RBO could be a good marker for packing of the matrix monolayer. On the other hand, the BAM image showed the morphology of not the matrix monolayer, but the adsorbed protein monolayer. Thus, the packing processes of the two could be distinguished. The present method is available for a protein that does not have visible absorption, such as bovine serum albumin (BSA). It is suggested that electrostatic interaction between matrix and protein molecules greatly affects the change in the morphology of the protein-adsorbed monolayer.

In situ observation of bovine serum albumin-adsorbed stearic acid monolayer by Brewster angle microscopy. Publishing Authors By Initials

H Tanaka,T Akatsuka,T Murakami,Y Ogoma,K Abe,Y Kondo,

For similar natural sciences: chemistry: chemistry, physical: surface properties research abstracts see: natural sciences: chemistry: chemistry, physical: surface properties research

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In situ observation of bovine serum albumin-adsorbed stearic acid monolayer by Brewster angle microscopy. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Journal of biochemistry

VOLUME: 121

Page Numbers: 206-11

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: Feb

YEAR: 1997

In situ observation of bovine serum albumin-adsorbed stearic acid monolayer by Brewster angle microscopy. Information

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LANGUAGE: eng

NlmUniqueID: 376600

In situ observation of bovine serum albumin-adsorbed stearic acid monolayer by Brewster angle microscopy. Keywords Mesh Terms:

KEYWORDS: Surface Properties

MESH TERMS: pharmacokinetics

Chemical & Substance for Abstract: In situ observation of bovine serum albumin-adsorbed stearic acid monolayer by Brewster angle microscopy. Information

Substance Name: octadecyl Rhodamine B chloride

Registry Number: 65603-19-2

Grant and Affiliation Information for In situ observation of bovine serum albumin-adsorbed stearic acid monolayer by Brewster angle microscopy.

AFFILIATION: Nippon Mektron Ltd., Ibaraki.

Country: JAPAN

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MEDLINETA: J Biochem

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