In situ hybridization to detect bandicoot papillomatosis carcinomatosis virus type 1 in biopsies from endangered western barred bandicoots (Perameles bougainville).

In situ hybridization to detect bandicoot papillomatosis carcinomatosis virus type 1 in biopsies from endangered western barred bandicoots (Perameles bougainville). Research Abstract Details 

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In situ hybridization to detect bandicoot papillomatosis carcinomatosis virus type 1 in biopsies from endangered western barred bandicoots (Perameles bougainville). Abstract Text:

Mark D Bennett,Lucy Woolford,Amanda J O'Hara,Kristin S Warren,Philip K Nicholls,Mark D Bennett,Lucy Woolford,Amanda J O'Hara,Kristin S Warren,Philip K Nicholls,Mark D Bennett,Lucy Woolford,Amanda J O'Hara,Kristin S Warren,Philip K Nicholls,Mark D Bennett,Lucy Woolford,Amanda J O'Hara,Kristin S Warren,Philip K Nicholls,

The western barred bandicoot (Perameles bougainville) is an endangered Australian marsupial species in which a papillomatosis and carcinomatosis syndrome occurs. Bandicoot papillomatosis carcinomatosis virus type 1 (BPCV1) is associated with the lesions of this progressively debilitating syndrome. Five digoxigenin-labelled DNA probes were generated for in situ hybridization (ISH) and the technique was optimized and performed on formalin-fixed paraffin-embedded (FFPE) biopsies. Staining of keratinocyte and sebocyte nuclei within lesions was achieved with all five probes. The sensitivity of ISH (76.9 %) surpassed that of PCR (30.8 %) for FFPE samples. The sensitivity of ISH varied from 81 % (papillomas) and 70 % (carcinoma in situ) to 29 % (squamous cell carcinomas). The specificity of the test was confirmed using an irrelevant probe and papillomas from other species. These results strengthen the association between BPCV1 and the western barred bandicoot papillomatosis and carcinomatosis syndrome and give insight into the biology of the virus-host interaction.

In situ hybridization to detect bandicoot papillomatosis carcinomatosis virus type 1 in biopsies from endangered western barred bandicoots (Perameles bougainville). Publishing Authors By Initials

MD Bennett,L Woolford,AJ O'Hara,KS Warren,PK Nicholls,MD Bennett,L Woolford,AJ O'Hara,KS Warren,PK Nicholls,MD Bennett,L Woolford,AJ O'Hara,KS Warren,PK Nicholls,MD Bennett,L Woolford,AJ O'Hara,KS Warren,PK Nicholls,

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In situ hybridization to detect bandicoot papillomatosis carcinomatosis virus type 1 in biopsies from endangered western barred bandicoots (Perameles bougainville). Journal Published:

PUBLICATION TYPE: Journal Article

Journal: The Journal of general virology

VOLUME: 89

Page Numbers: 419-23

Journal Abbreviation: J. Gen. Virol.

ISSN: 0022-1317

DAY: 16

MONTH: Feb

YEAR: 2008

In situ hybridization to detect bandicoot papillomatosis carcinomatosis virus type 1 in biopsies from endangered western barred bandicoots (Perameles bougainville). Information

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LANGUAGE: eng

NlmUniqueID: 77340

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AFFILIATION: School of Veterinary and Biomedical Sciences, Murdoch University, South Street, Murdoch, Western Australia 6150, Australia.

Country: England

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MEDLINETA: J Gen Virol

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